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BrightLine® Laser Dichroic Beamsplitters

Steeper. Wider. Flatter.

  • λ/5 P-V Reflected Wavefront Error (RWE)  on 3 mm
  • 1λ P-V Reflected Wavefront Error (RWE) on 1 mm
  • Minimal reflected wavefront distortion for large diameter illumination beams
  • The steepest edges for higher throughput and signal collection
  • Wider reflection bands — into UV for photoactivation and super-resolution techniques
  • Wider transmission regions — into IR to 1200 or 1600 nm

Semrock’s industry leading laser dichroic beamsplitters just got flatter. We’re setting a new standard for super-resolution microscopy with λ/5 P-V RWE on our new 3 mm thick dichroics and improved 1λ P-V RWE on our improved 1 mm dichroics. Each one comes with our Semrock guaranteed steepest edges, short wavelength reflectivity down to 350 nm, and long wavelength transmission optimized out to 1200 nm or 1600 nm.

tableSuper-resolution imaging systems are highly sensitive to optical wavefront distortion and demand the highest quality components. In order to address the needs of this market, Semrock has introduced industry leading laser dichroic beamsplitters with λ/5 RWE to minimize the reflected wavefront distortion, thereby maximizing both the signal and the signal-to-noise ratio in super-resolution microscopes.

For setups in which maximizing the switching speed or minimizing the beam shift in transmission is critical, Semrock’s 1 mm thick laser dichroic beamsplitters have been significantly improved to 1λ RWE (~255 m radius of curvature). These industry leading optics are compatible with popular microscopy filter cubes and improve the performance of laser based confocal and TIRF illumination systems. They are also ideal for reflection of imaging beams in conventional structured-illumination techniques as well as patterned illumination systems for localized photo-activation. These dichroic beamsplitters allow the use of much larger diameter illumination beams, offering researchers and instrument developers more flexibility in system design with no compromise to overall performance.  For more detailed background and information, please review our Tech Notes titled “BrightLine® Laser Dichroic Beamsplitters” & “Practical Flatness”. 

Flatness

Product Code

Substrate Thickness

Radius of Curvature

Application Specification

1λ P-V RWE

Di03-Rxxx-t1-25x36

1.05 mm

~ 255 m

Transmission: does not cause significant aberrations to a transmitted beam over the full clear aperture

Reflected Laser Beam: contributes less than one Rayleigh Range of shift in focus (relative to a perfectly flat mirror) at the focal plane of a lens after reflecting a laser beam with a diameter up to 10 mm

Reflected Imaging Beam: contributes less than 1.5 x Airy Disk diameter to the RMS spot size of a reflected beam with a diameter up to 16.7 mm

λ/5 P-V RWE

Di03-Rxxx-t3-25x36

3.0 mm

~ 1275 m

Transmission: does not cause significant aberrations to a transmitted beam over the full clear aperture

Reflected Laser Beam: contributes less than one Rayleigh Range of shift in focus (relative to a perfectly flat mirror) at the focal plane of a lens after reflecting a laser beam with a diameter up to 22.5 mm

Reflected Imaging Beam: contributes less than 1.5 x Airy Disk diameter to the RMS spot size of a reflected beam with a diameter up to 37.0 mm

 

Super-resolution Microscopy Cubes
Filter sets with guaranteed 1λ P-V RWE from cube mounted 1mm thick dichroic beamsplitters are available.

 

Laser Dichroic Beamsplitters

Reflected Laser Wavelengths

Extended Average Reflection Band (nm)

Absolute Reflection Band (nm)

Average Transmission Band (nm)

 RWE
1 mm Thickness
Filter Part Number 

λ/5 RWE
3 mm Thickness 
Filter Part Number

Availability

375.0 ± 3 nm 405.0 ± 5 nm

> 90% 350.0 – 372.0

> 94% 372.0 – 410.0

> 93% 417.4 – 1200

Di03-R405-t1-25x36

Di03-R405-t3-25x36

In Stock

440 +3/-1 nm

442.0 nm

457.9 nm

> 90% 350.0 – 439.0

> 94% 439.0 – 457.9

> 93% 466.1 – 1200

Di03-R442-t1-25x36

Di03-R442-t3-25x36

In Stock

473.0 ± 2 nm 488.0 +3/-2 nm

> 90% 350.0 – 471.0

> 94% 471.0 – 491.0

> 93% 499.8 – 1200

Di03-R488-t1-25x36

Di03-R488-t3-25x36

In Stock

505.0 nm

514.5 nm

515.0 nm

> 90% 350.0 – 505.0

> 94% 505.0 – 515.0

> 93% 524.3 – 1200

Di03-R514-t1-25x36

Di03-R514-t3-25x36

In Stock

514.5 nm

532.0 nm

> 90% 350.0 – 514.0

> 94% 514.0 – 532.0

> 93% 541.6 – 1200

Di03-R532-t1-25x36

Di03-R532-t3-25x36

In Stock

561.4 nm

568.2 nm

> 90% 350.0 – 554.0

> 94% 554.0 – 568.2

> 93% 578.4 – 1200

Di03-R561-t1-25x36

Di03-R561-t3-25x36

In Stock

593.5 nm

594.0 -0/+.3 nm

594.1 nm

> 90% 350.0 – 593.5

> 94% 593.5 – 594.3

> 93% 605.0 – 1200

Di03-R594-t1-25x36

Di03-R594-t3-25x36

In Stock

632.8 nm

635.0 +7/-3 nm

647.1 nm

> 90% 350.0 – 632.8

> 94% 632.8 – 641.7

> 93% 658.8 – 1200

Di03-R635-t1-25x36

Di03-R635-t3-25x36

In Stock

785.0 ± 5 nm

> 90% 350.0 – 780.0

> 94% 780.0 – 790.0

> 93% 804.3 – 1600

Di03-R785-t1-25x36

Di03-R785-t3-25x36

In Stock

473 ± 2 nm, 488 +3/-2 nm

559 +5/-3, 561.4, 568.2 nm

> 90% 350.0 – 471.0

> 94% 471.0 – 491.0

> 94% 559.0 – 568.2

> 93% 503.3 – 543

> 93% 582.4 – 1200

Di03-R488/561-t1-25x36

Di03-R488/561-t3-25x36

In Stock

375 ± 3, 405 ± 5 nm

473 +2/-0, 488 +3/-2 nm

532.0 nm

632.8, 635 +7/-0, 647.1 nm

> 90% 350.0 – 370.0

> 94% 370.0 – 410.0

> 94% 473.0 – 491.0

> 94% 530.5 – 533.5

> 94% 632.8 – 641.7

> 93% 426 – 462

> 93% 502.5 – 518.5

> 93% 550 – 613

> 93% 663 – 1200

Di03-R405/488/532/635-t1- 25x36

Di03-R405/488/532/635-t3- 25x36

In Stock

375 ± 3, 405 ± 5 nm

473 +2/-0, 488 +3/-2 nm

559 +5/-3, 561.4, 568.2 nm

632.8, 635 +7/-0, 647.1 nm

> 90% 350.0 – 370.0

> 94% 370.0 – 410.0

> 94% 473.0 – 491.0

> 94% 559.0 – 568.2

> 94% 632.8 – 641.7

> 93% 426 – 462

> 93% 502.5 – 544.5

> 93% 582 – 617.5

> 93% 663 – 1200

Di03-R405/488/561/635- t1-25x36

Di03-R405/488/561/635- t3-25x36

In Stock

Click to Download the Brightline® Laser Dichroic Beamsplitter Data Sheet

1/10 Wave 0.1Wave 1/10Wave 1/2 Wave 0.5Wave 1/2Wave Laser Flat Dichroic Beamsplitter Ultra-Flat Laser-Flat Di03-R FDi03-R Super-resolution P-V Flatness optical-wavefront-distortion reflected-wavefront-distortion TIRF TIRF-illumination-systems PALM STORM structured-illumination-microscopy SIM Rayleigh Range Airy Disk Di03-R405 R405 FDi03-R405 Di03-R488 R488 FDi03-R488 Di03-R635 R635 FDi03-R635 Di03-R405/488/561/635 FDi03-R405/488/561/635 R405/488/561/635 Di03-R405-25x36 FDi03-R405-25x36 Di03-R488-25x36 FDi03-R488-25x36 Di03-R635-25x36 FDi03-R635-25x36 Di03-R405/488/561/635-25x36 FDi03-R405/488/561/635-25x36 Di03-R532 R532 FDi03-R532 Di03-R785 R785 FDi03-R785 Di03-R405/488/532/635 FDi03-R405/488/532/635 Di03-R532-25x36 FDi03-R532-25x36 Di03-R785-25x36 FDi03-R785-25x36 Di03-R405/488/532/635-25x36 FDi03-R405/488/532/635-25x36 Di03-R442 R442 FDi03-R442 Di03-R514 R514 FDi03-R514 Di03-R561 R561 FDi03-R561 Di03-R488/561 FDi03-R488/561 R488/561 Di03-R442-25x36 FDi03-R442-25x36 Di03-R514-25x36 FDi03-R514-25x36 Di03-R561-25x36 FDi03-R561-25x36 Di03-R488/561-25x36 FDi03-R488/561-25x36 Di03-R594 R594 FDi03-R594