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BrightLine® single-band filter set, optimized for mCherry and other like fluorophores, illuminated with LED based light sources

Semrock Set / Fluorophore Graphing

Part Number: LED-mCherry-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: LED-mCherry-A

BrightLine® single-band filter set, optimized for mCherry and other like fluorophores, illuminated with LED based light sources

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring mCherry (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LED-mCherry-A-000 (Standard size set, fits most microscopes2)$825Confirmed AROAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-578/21-25$355
Single Band Emitter25 mm x 3.5 mmFF02-641/75-25$325
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF596-Di01-25x36$335
Click to expand/collapse details LED-mCherry-A-000-ZERO (Standard size -ZERO pixel set)$924Confirmed AROAdd To Cart
Click to expand/collapse details LED-mCherry-A-L01-ZERO (Leica 'Large' -ZERO pixel set)$924Confirmed AROAdd To Cart
Click to expand/collapse details LED-mCherry-A-U02 (Olympus large-size (FX) set for U-M710I)$1,370Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LED-mCherry-A-NQF (set mounted in cube)$1,220Confirmed AROAdd To Cart
Click to expand/collapse details LED-mCherry-A-NQF-ZERO (-ZERO pixel set mounted in cube)$1,319Confirmed AROAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LED-mCherry-A-NTE (set mounted in cube)$1,220Confirmed AROAdd To Cart
Click to expand/collapse details LED-mCherry-A-NTE-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LED-mCherry-A-OMF (set mounted in cube)$1,260Confirmed AROAdd To Cart
Click to expand/collapse details LED-mCherry-A-OMF-ZERO (-ZERO pixel set mounted in cube)$1,359Confirmed AROAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LED-mCherry-A-OFF (set mounted in cube)$1,260Confirmed AROAdd To Cart
Click to expand/collapse details LED-mCherry-A-OFF-ZERO (-ZERO pixel set mounted in cube)$1,359Confirmed AROAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details LED-mCherry-A-OFX (set mounted in cube)$2,065Confirmed AROAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LED-mCherry-A-ZHE (set mounted in cube)$1,100Confirmed AROAdd To Cart
Click to expand/collapse details LED-mCherry-A-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,199Confirmed AROAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LED-mCherry-A-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,274Confirmed AROAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LED-mCherry-A-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,359Confirmed AROAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details LED-mCherry-A-AMF-ZERO (-ZERO pixel set mounted in cube)$1,389Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556153-Star
5-ROX (carboxy-X-rhodamine)5786045-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5685796035-Star
Alexa Fluor® 5945906185-Star
APC (Allophycocyanin)6516601-Star
AsRed 25765925-Star
ATTO 5655635921-Star
ATTO 5905946245-Star
ATTO 5946016243-Star
ATTO 6106146341-Star
BODIPY TR-X phallacidin5906215-Star
Calcium Crimson5896095-Star
CellTrace BODIPY TR methyl ester5986253-Star
CellTracker Red CMTPX5866145-Star
DyLight 5945936185-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Red fluorescent microspheres5776075-Star
HCS LipidTOX Deep Red neutral lipid stain6356521-Star
HCS LipidTOX Red neutral lipid stain5826165-Star
HCS LipidTOX Red phospholipidosis5846085-Star
HiLyte Fluor™ 5945926165-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956133-Star
LysoTracker Red5735923-Star
MitoTracker™ Red5785985-Star
Nile blue-EtOH6316601-Star
Nile red-phospolipid5536373-Star
Propidium Iodide (PI)3056173-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Qdot® 655 Nanocrystals3006541-Star
Red 5805806035-Star
Rhodamine Red-X5735911-Star
SNARF (carboxy) 514 Excitation pH95766385-Star
SNARF-1 488nm (ph 9.0)5766385-Star
SNARF-1 514nm (ph 9.0)5766385-Star
Sulforhodamine 101-EtOH5785931-Star
SYTO 176196381-Star
SYTO 616186501-Star
Texas Red dextran5926135-Star
Texas Red DHPE5846085-Star
Texas Red-X®5956135-Star
X-Rhod-1 Indicator5806015-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.

LED-mCherry-A mCherry LED 578/21 641/75 596 FF02-641/75 FF596-Di01 FF01-578/21 FF596 TexasRed TxRed Lumencor Sola Spectra-X CoolLED pE-4000 pE-300-White X-Cite Turbo XLED1 110LED-Broadband 120LED-Broadband 5-ROX ROX ATTO590 Alexa-Fluor-568 Alexa568 AF568 Alexa-Fluor-594 Alexa594 AF594 mPlum mRFP1 Filter Filters Set Sets LED-mCherry-A-000 LED-mCherry-A-000-ZERO LED-mCherry-A-L01-ZERO LED-mCherry-A-U02 LED-mCherry-A-NQF LED-mCherry-A-NQF-ZERO LED-mCherry-A-NTE LED-mCherry-A-NTE-ZERO LED-mCherry-A-OMF LED-mCherry-A-OMF-ZERO LED-mCherry-A-OFF LED-mCherry-A-OFF-ZERO LED-mCherry-A-OFX LED-mCherry-A-ZHE LED-mCherry-A-ZHE-ZERO LED-mCherry-A-LDMP-ZERO LED-mCherry-A-LDMK-ZERO LED-mCherry-A-AMF-ZERO

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