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BrightLine Basic™ single-band filter set, optimized for Calcofluor White and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: CFW-BP01-Clinical-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 5 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 3503434413-Star
Alexa Fluor® 4054014221-Star
AMCA (Aminomethylcoumarin)3504481-Star
ATTO 3903924771-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804403-Star
Calcofluor White3514445-Star
Cascade Blue™4014191-Star
CellTrace calcein violet4004523-Star
DiFMU-pH 9.03584501-Star
DyLight 4053984203-Star
HiLyte Fluor™ 4054034281-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
LysoSensor Blue3744243-Star
LysoTracker Blue3734221-Star
Marina Blue3634611-Star
Pacific Blue™4044553-Star
Vybrant DyeCycle Violet3694371-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Journal Article
Optical Filters for Clinical Fluorescence MicroscopyAtul Pradhan, PhD, Clinical Lab Products, January 2007
Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
Clinical MicroscopyAn overview of clinical microscopy, including solutions from Semrock and additional learning resources.

CFW-BP01-Clinical Calcofluor CalcofluorWhite CFW-BP01-Clin CFW-Di01-Clin CFW-Ex01-Clin

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