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Orientation of Filters in a Microscope

Because BrightLine filters are so durable, you can easily populate your own cubes, sliders, and filter wheels without fear of damaging the filters. To obtain the optimal performance, filters should be oriented properly. The diagrams below explain the proper technique.

 

Proper Orientation of All Filters

Orient the exciter and emitter with the arrow on the side of the aluminum ring pointing in the direction light propagation – from the light source to the dichroic for the exciter and from the dichroic to eye or camera for the emitter. The reflective side of the dichroic should face the light source and the sample. 

Microscope filter orientation

 

Determining the Orientation of a Dichroic Beamsplitter

Marked Dichroics
To determine the reflective side of the dichroic, lay the filter on a table with the short side closest to you. The reflective side is up when the logo, corner chamfer or etched line is in either the far left corner or the near right corner. 

 

Marked dichroic orientation

 

  

Unmarked Dichroics
When viewing the dichroic with the reflective coating side down, you can see a double reflection of a bright object and the thickness of the filter at the far edge is apparent.  When viewing the dichroic with the reflective coating side up, you can see a predominately single reflection of a bright object and the thickness of the filter at the far edge is not visible.

Unmarked dichroic coated side down

Dichroic reflective coating side down

Orientation marking Arrow-direction Arrow direction-of-light