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Mercury Line Spectroscopy

Mercury arc lamps continue to be one of the most popular excitation sources for fluorescence microscopy because of the intense brightness they provide.  Semrock offers a line of MaxLamp® mercury line filters that are designed to isloate particular mercury lines while withstanding the intense UV radiation.  These ultrahigh-performance MaxLamp mercury line filters are ideal for use with high-power mercury arc lamps for applications including spectroscopy, optical metrology, and photolithography mask-aligner and stepper systems. Maximum throughput is obtained by careful optimization of the filter design to allow for use in a variety of different applications. The non-absorbing blocking ensures that all other mercury lines as well as intra-line intensity are effectively eliminated. 

Learn more about mercury line spectroscopy:

Technical Notes

Introduction to Fluorescence Filters

Microscope Cube Assembly Instructions

Orientation of Filters in a Microscope

Multiband Filter Set Terminology

What is Pixel Shift?

Application Notes

UV Fluorescence Imaging

Related Publications

Bringing Superresolution to Fluorescence Microscopy (May/June 2010), BioPhotonics

New Optical Filters Improve High-Speed Multicolor Fluorescence Imaging (March 2006), BioPhotonics

Optical Filters Impact Fluorescence Fidelity (November 2003), BioPhotonics

From the Semrock White Paper Series

Flatness of Dichroic Beamsplitters Affects Focus and Image Quality

Additional Resources

Fundamentals of Mercury Arc Lamps by Zeiss

Mercury-Line-Spectroscopy