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BrightLine® Image-splitting Dichroic Beamsplitters

Semrock’s image-splitting dichroic beamsplitters are the industry standard enabling simultaneous multi-color imaging applications. The spectral edges of these filters are optimized for imaging of popular fluorophore-pairs providing maximum signal throughput, while maintaining minimal wavefront distortion in reflection and transmission thereby maximizing contrast and resolution of the overall imaging system.

                                             


Lack of sufficient flatness of image-splitting dichroic beamsplitters can introduce significant optical aberrations compromising resolution and contrast. For an in-depth review on this topic read our following technical articles.

BrightLine® Image-splitting  dichroic beamsplitter for Super-resolution Microscopy

  • λ/5 P-V Reflected Wavefront Error (RWE)  on 3 mm
  • Spectral edges are optimized for popular fluorophore pairs
  • Super-resolution microscopy for large diameter imaging beams
  • Thicker filter substrate provides a robust solution for mounting
  • Wide reflection bands — into UV & wide transmission regions — to 1200 nm in IR      

        

 

Flatness / RWE Clasification

Example Applications

Nominal Radius of
Curvature

Maximum Reflected
Beam Diameter, mm

Reflected Wavefront
Error at
632.8 nm, PV

Dichroic Family, and
Example Part Numbers

Image-splitting Splitting of emission signal on a pixel based detector ~ 1275 meters 37 <0.2λ BrightLine Image-splitting
(FF509-FDi02-t3-)

 

Fluorophore
Pair to Split

Reflection Band (nm)

Transmission Band (nm)

Filter Part Number  

 Availability

DAPI / FITC
BFP / GFP

350 – 475 492.3 – 1200 FF484-FDi02-t3-25x36 Q1-2018

CFP / YFP

350 – 500 518.3 – 1200 FF509-FDi02-t3-25x36 In Stock

GFP / mOrange

350 – 528.4 547.7 – 1200 FF538-FDi02-t3-25x36 Q1-2018

YFP / dTomato

350 – 550 570.1 – 1200 FF560-FDi02-t3-25x36 In Stock

GFP / mCherry
FITC / TexasRed

 350 – 570 590.8 – 1200  FF580-FDi02-t3-25x36   In Stock

Cy3 / Cy5

350 – 629.5 652 – 1200 FF640-FDi02-t3-25x36 In Stock

TexasRed / Cy5

350 – 650 673.7 – 1200 FF662-FDi02-t3-25x36 Q1-2018

 

  • BrightLine® Image-splitting Dichroic Beamsplitter for Standard Microscopy

    • Ideal solution for standard Image-splitting applications 
    • Spectral edges are optimized for popular fluorophore pairs
    • Wide reflection bands — into UV & wide transmission regions —to 950 nm in IR
         

 

Flatness / RWE Clasification

Example Applications

Nominal Radius of
Curvature

Maximum Reflected
Beam Diameter, mm

Reflected Wavefront
Error at
632.8 nm, PV

Dichroic Family, and
Example Part Numbers

Image-splitting Splitting of emission signal on a pixel based detector ~ 100 meters 10 < 2λ BrightLine Image-splitting
(FF509-FDi01-)

 

Fluorophore
Pair to Split

Reflection Band (nm)

Transmission Band (nm)

Filter Part Number  

 Availability

DAPI / FITC
BFP / GFP

350 – 475 492.3 – 950 FF484-FDi01-25x36 In Stock

CFP / YFP

350 – 500 518.3 – 950 FF509-FDi01-25x36 In Stock

GFP / mOrange

350 – 528.4 547.7 – 950 FF538-FDi01-25x36 In Stock

YFP / dTomato

350 – 550 570.1 – 950 FF560-FDi01-25x36 In Stock

GFP / mCherry
FITC / TexasRed

 350 – 570 590.8 – 950  FF580-FDi01-25x36  In Stock

Cy3 / Cy5

350 – 629.5 652 – 950 FF640-FDi01-25x36 In Stock

TexasRed / Cy5

350 – 650 673.7 – 950 FF662-FDi01-25x36 In Stock

Click to Download the Brightline® Image-splitting Dichroic Beamsplitter Data Sheet