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SetDetails

BrightLine® full-multiband filter set, optimized for Cyan & Yellow Fluorescent Proteins and other like fluorophores, illuminated with Lumencor CELESTA light engine

Semrock Set / Fluorophore Graphing

Part Number: CELESTA-CFP/YFP-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: CELESTA-CFP/YFP-A

BrightLine® full-multiband filter set, optimized for Cyan & Yellow Fluorescent Proteins and other like fluorophores, illuminated with Lumencor CELESTA light engine

This "full-multiband" filter set allows for simultaneous direct viewing or imaging of CFP (Cyan Fluorescent Protein) and YFP (Yellow Fluorescent Protein) (and other key fluorophores – see complete list above). This set has been optimized to be illuminated with Lumencor CELESTA light engine® utilizing 445 nm & 518 nm wavelengths. This set has been designed to deliver significant fluorescence signal improvements for the CELESTA light engine compared with sets designed for standard broadband light sources such as mercury or xenon arc lamps or other LED-based light engines. The complete set is comprised of a dual-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details CELESTA-CFP/YFP-A-000 (Standard size set, fits most microscopes2)$1,365Confirmed AROAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Quad Band Exciter25 mm x 5.0 mmFF01-449/520-25$495
Quad Band Emitter25 mm x 3.5 mmFF01-484/561-25$495
Quad Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF471/539-Di01-25x36$425
Click to expand/collapse details CELESTA-CFP/YFP-A-000-ZERO (Standard size -ZERO pixel set)$1,464Confirmed AROAdd To Cart
Click to expand/collapse details CELESTA-CFP/YFP-A-L01-ZERO (Leica 'Large' -ZERO pixel set)$1,464Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details CELESTA-CFP/YFP-A-NQF (set mounted in cube)$1,760Confirmed AROAdd To Cart
Click to expand/collapse details CELESTA-CFP/YFP-A-NQF-ZERO (-ZERO pixel set mounted in cube)$1,859Confirmed AROAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details CELESTA-CFP/YFP-A-NTE (set mounted in cube)$1,760Confirmed AROAdd To Cart
Click to expand/collapse details CELESTA-CFP/YFP-A-NTE-ZERO (-ZERO pixel set mounted in cube)$1,859Confirmed AROAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details CELESTA-CFP/YFP-A-OMF (set mounted in cube)$1,800Confirmed AROAdd To Cart
Click to expand/collapse details CELESTA-CFP/YFP-A-OMF-ZERO (-ZERO pixel set mounted in cube)$1,899Confirmed AROAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details CELESTA-CFP/YFP-A-OFF (set mounted in cube)$1,800Confirmed AROAdd To Cart
Click to expand/collapse details CELESTA-CFP/YFP-A-OFF-ZERO (-ZERO pixel set mounted in cube)$1,899Confirmed AROAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details CELESTA-CFP/YFP-A-ZHE (set mounted in cube)$1,640Confirmed AROAdd To Cart
Click to expand/collapse details CELESTA-CFP/YFP-A-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,739Confirmed AROAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6
Click to expand/collapse details CELESTA-CFP/YFP-A-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,814Confirmed AROAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details CELESTA-CFP/YFP-A-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,899Confirmed AROAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Cyan
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Acridine Yellow4705501-Star
Aqua 4314304785-Star
ATTO 4254364845-Star
ATTO 4654535071-Star
BD Horizon V5004105001-Star
BOBO™-14614843-Star
Cerulean4344735-Star
CFP (ECFP)4334755-Star
DY-4154184703-Star
ECFP4354755-Star
mBBr+GSH3944901-Star
mTFP14624925-Star
POPO-14334575-Star
PO-PRO-14334561-Star
SpectrumAqua4344815-Star
SYTO 454514853-Star
SYTOX Blue4444705-Star
TagCFP4584805-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Yellow
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925181-Star
6-carboxyrhodamine 6G5255555-Star
6-JOE5205485-Star
Acridine Orange (+DNA)5025265-Star
Alexa Fluor® 4884995201-Star
Alexa Fluor® 5005035251-Star
Alexa Fluor® 5145185435-Star
Alexa Fluor® 5325345531-Star
ATTO 4885005253-Star
ATTO 4954955275-Star
ATTO 5205165385-Star
ATTO 5325015451-Star
BODIPY R6G5285473-Star
Calcein4945141-Star
Calcium Green-15075295-Star
CyQUANT GR-DNA5025233-Star
DAF-FM-NO4955191-Star
Dronpa5025161-Star
DY-505-Phalloidin5035305-Star
DyLight 4884935181-Star
Eosin5255465-Star
FITC (Fluorescein)4955191-Star
FlAsH-CCPFCC5115305-Star
Fluo-35065275-Star
Fluo-44945161-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895171-Star
Fluoro-Emerald4945183-Star
FluoSpheres Orange fluorescent microspheres5405591-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
FM 1-434735791-Star
Gold 5255215515-Star
Green 4964965201-Star
Green 5005015243-Star
HCS LipidTOX Green phospholipidosis5045365-Star
HiLyte Fluor™ 4884975263-Star
JO-JO-1, JO-PRO-15305443-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075315-Star
mHoneyDew4785625-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135325-Star
PicoGreen®5025221-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
Rhodamine 1104975191-Star
Rhodamine 1235075293-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
Sodium Green5075325-Star
SpectrumGreen4975381-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYBR Safe DNA gel stain-DNA5095263-Star
SYTO 115065253-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TO-PRO-15155315-Star
TOTO-15145315-Star
TurboYFP5255383-Star
Venus5165285-Star
VIC5305501-Star
Vybrant DyeCycle Green5065345-Star
Vybrant DyeCycle Orange5195633-Star
YFP (yellow GFP)5135305-Star
ZsYellow15295393-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



CELESTA-CFP/YFP-A CFP/YFP CFP YFP Cerulean Venus Full-Multiband Dual-Band Dual Filter Filters Set Sets Dual FF01-449/520 449/520 FF01-484/561 484/561 FF471/539-Di01 471/539 Lumencor Celesta CELESTA ZIVA Ziva CELESTA-CFP/YFP-A-000 CELESTA-CFP/YFP-A-000-ZERO CELESTA-CFP/YFP-A-L01-ZERO CELESTA-CFP/YFP-A-NQF CELESTA-CFP/YFP-A-NQF-ZERO CELESTA-CFP/YFP-A-NTE CELESTA-CFP/YFP-A-NTE-ZERO CELESTA-CFP/YFP-A-OMF CELESTA-CFP/YFP-A-OMF-ZERO CELESTA-CFP/YFP-A-OFF CELESTA-CFP/YFP-A-OFF-ZERO CELESTA-CFP/YFP-A-ZHE CELESTA-CFP/YFP-A-ZHE-ZERO CELESTA-CFP/YFP-A-LDMP-ZERO CELESTA-CFP/YFP-A-LDMK-ZERO

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