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BrightLine® Sedat filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores, illuminated with LED-based light engines

Semrock Set / Fluorophore Graphing

Part Number: LED-DA/FI/TX-3X3M-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: LED-DA/FI/TX-3X3M-B

BrightLine® Sedat filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores, illuminated with LED-based light engines

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 7-filter "Sedat" quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, and Texas Red (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LED-DA/FI/TX-3X3M-B-000 (Standard size set, fits most microscopes2)$2,075Ships in 2 daysAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-378/52-25$355
Single Band Exciter25 mm x 5.0 mmFF01-474/27-25$355
Single Band Exciter25 mm x 5.0 mmFF03-575/25-25$305
Single Band Emitter25 mm x 3.5 mmFF01-432/36-25$355
Single Band Emitter25 mm x 5.0 mmFF02-525/40-25$355
Single Band Emitter25 mm x 3.5 mmFF02-641/75-25$305
Triple Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF409/493/596-Di02-25x36$465
Click to expand/collapse details LED-DA/FI/TX-3X3M-B-L01 (Leica 'Large' set)$2,075Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LED-DA/FI/TX-3X3M-B-NQF (set mounted in cube)$2,470Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LED-DA/FI/TX-3X3M-B-NTE (set mounted in cube)$2,470Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LED-DA/FI/TX-3X3M-B-OMF (set mounted in cube)$2,510Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LED-DA/FI/TX-3X3M-B-OFF (set mounted in cube)$2,510Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LED-DA/FI/TX-3X3M-B-ZHE (set mounted in cube)$2,350Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LED-DA/FI/TX-3X3M-B-LDMK (set mounted in cube)$2,425Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LED-DA/FI/TX-3X3M-B-LDMP (set mounted in cube)$2,510Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus and Zeiss microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 4054014225-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804405-Star
BV421 - BD Horizon Brilliant™ Violet 4214074211-Star
Cascade Blue™4014195-Star
CellTrace calcein violet4004523-Star
DAPI3594615-Star
DyLight 4053984205-Star
HiLyte Fluor™ 4054034285-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034551-Star
LysoSensor Blue3744243-Star
Marina Blue3634611-Star
Pacific Blue™4044551-Star
sgBFP™3874513-Star
SpectrumBlue4054493-Star
TagBFP4024571-Star
Vybrant DyeCycle Violet3694371-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-FAM (5-carboxyfluorescein)4925185-Star
Acridine Orange (+DNA)5025261-Star
Acridine Yellow4705501-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995201-Star
Alexa Fluor® 5005035251-Star
AmCyan14584895-Star
ATTO 4654535071-Star
ATTO 4885005251-Star
ATTO 4954955273-Star
BOBO™-14614841-Star
BODIPY FL5055121-Star
BODIPY FL-MeOH5025111-Star
Calcein4945143-Star
CFP24905105-Star
Cy2™4925075-Star
CyQUANT GR-DNA5025231-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915075-Star
DiO4755005-Star
Dronpa5025161-Star
DY-505-Phalloidin5035301-Star
ecliptic pHluorin pH5.54735075-Star
Emerald4915115-Star
evoglow-Bs14494961-Star
FITC (Fluorescein)4955195-Star
Fluo-44945165-Star
Fluorescein dextran5015241-Star
Fluorescein-pH 8.04895171-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
GFP (EGFP)4895115-Star
Green 4964965203-Star
Green 5005015241-Star
HCS LipidTOX Green phospholipidosis5045361-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoSensor Green4485023-Star
LysoTracker Green5015093-Star
LysoTracker Yellow HCK-1234885651-Star
mHoneyDew4785623-Star
MitoTracker™ Green4905125-Star
mTFP14624921-Star
mWasabi4935093-Star
NBD-X (MeOH)4675385-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
PicoGreen®5025221-Star
PKH674885005-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
Rhodamine 1104975193-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
sgGFP™ (super glow GFP)4725065-Star
Sodium Green5075321-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895205-Star
SYTO 94835005-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
TagCFP4584801-Star
TOTO-15145311-Star
TurboGFP4825035-Star
Vybrant DyeCycle Green5065341-Star
wtGFP (wild type GFP, non-UV excitation)4745095-Star
YO-PRO-14915065-Star
YOYO-14915085-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825951-Star
5-carboxynapthofluorescein (pH 10)5556151-Star
5-ROX (carboxy-X-rhodamine)5786045-Star
7-AAD (7-aminoactinomycin D)5486483-Star
Alexa Fluor® 5685796035-Star
Alexa Fluor® 5945906185-Star
Alexa Fluor® 6606636911-Star
AsRed 25765923-Star
ATTO 5655635921-Star
ATTO 5905946243-Star
ATTO 5946016241-Star
BOBO™-35706055-Star
BODIPY TR-X phallacidin5906215-Star
Calcium Crimson5896095-Star
Carboxynaphthofluorescein5996743-Star
CellTrace BODIPY TR methyl ester5986253-Star
CellTracker Red CMTPX5866145-Star
Cy3.5™5785911-Star
DDAO6486573-Star
DY-5905825993-Star
DY-6306346561-Star
DyLight 5945936185-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Red fluorescent microspheres5776075-Star
FM 4-645057251-Star
HcRed15886183-Star
HCS LipidTOX Red neutral lipid stain5826165-Star
HCS LipidTOX Red phospholipidosis5846085-Star
HiLyte Fluor™ 5945926163-Star
KFP-Red5755993-Star
LDS 7515607115-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956131-Star
LysoTracker Red5735921-Star
mApple5665941-Star
mCherry5876105-Star
MitoTracker™ Red5785983-Star
mPlum5896495-Star
mRFP15847005-Star
mRuby5586053-Star
mStrawberry5745963-Star
Nile blue-EtOH6316601-Star
Nile red-phospolipid5536373-Star
Orange 5525525741-Star
PerCP4906771-Star
Propidium Iodide (PI)3056171-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Qdot® 655 Nanocrystals3006541-Star
Qdot® 705 Nanocrystals3007021-Star
Qdot® 800 Nanocrystals3007921-Star
ReAsH-CCPGCC5926065-Star
Red 5805806035-Star
Rhodamine Red-X5735911-Star
SNARF (carboxy) 514 Excitation pH95766385-Star
SNARF-1 488nm (ph 9.0)5766385-Star
SNARF-1 514nm (ph 9.0)5766385-Star
SpectrumRed5876151-Star
Sulforhodamine 101-EtOH5785931-Star
SYTO 176196381-Star
SYTO 616186501-Star
Texas Red dextran5926133-Star
Texas Red DHPE5846085-Star
Texas Red-X®5956135-Star
TurboFP635(Katushka)5916385-Star
X-Rhod-1 Indicator5806015-Star
YO-PRO-36136291-Star
YOYO-36116311-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



LED-DA/FI/TX-3X3M-B LED 3X3M DAPI FITC TexasRed TxRed DAPI/FITC/TexasRed DAPI/FITC/TxRed DA/FI/TX Sedat LED 378/52 FF01-378/52 474/27 FF01-474/27 575/25 FF03-575/25 433/24 FF01-433/24 525/45 FF01-525/45 641/75 FF02-641/75 409/493/596 FF409/496/596-Di02 FF409/496/596 Lumencor Sola Spectra-X CoolLED pE-4000 pE-300-White X-Cite Turbo XLED1 110LED-Broadband 120LED-Broadband Filter Filters Set Sets LED-DA/FI/TX-3X3M-B-000 LED-DA/FI/TX-3X3M-B-L01 LED-DA/FI/TX-3X3M-B-NQF LED-DA/FI/TX-3X3M-B-NTE LED-DA/FI/TX-3X3M-B-OMF LED-DA/FI/TX-3X3M-B-OFF LED-DA/FI/TX-3X3M-B-ZHE LED-DA/FI/TX-3X3M-B-LDMP LED-DA/FI/TX-3X3M-B-LDMK

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