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BrightLine^® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores, illuminated with LED-based light engines

Semrock Set / Fluorophore Graphing

Part Number: LED-DA/FI/TX-3X-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Filters and Pricing
Specifications
Fluorophore
Technical Info

BrightLine^® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores, illuminated with LED-based light engines

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 5-filter "Pinkel" triple-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, and Texas Red, (or other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines, including 365 nm DAPI LEDs, compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter and a triple-band emitter (these can be offered assembled in a microscope cube), plus three "no burn-out" single-band exciters that are intended to be mounted in the LED-based light engines, or in an exciter filter wheel or slider.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

Part Number

Price¹

Stock Status

Quantity

LED-DA/FI/TX-3X-B-000 (Standard size set, fits most microscopes²)

$1,655

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465

LED-DA/FI/TX-3X-B-000-ZERO (Standard size -ZERO pixel set)

$1,754

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
-ZERO set certification		-ZERO	$99

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	23.3 mm x 2.0 mm	FF01-432/523/702-23.3-D	$465
Triple Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF409/493/596-Di02-22x29	$465

LED-DA/FI/TX-3X-B-L01-ZERO (Leica 'Large' -ZERO pixel set)

$1,754

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	23.3 mm x 2.0 mm	FF01-432/523/702-23.3-D	$465
Triple Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF409/493/596-Di02-22x29	$465
-ZERO set certification		-ZERO	$99

LED-DA/FI/TX-3X-B-S01 (Sutter threaded set)

$1,715

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-378/52-25-STR	$375
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-474/27-25-STR	$375
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF03-575/25-25-STR	$345
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465

LED-DA/FI/TX-3X-B-S01-ZERO (Sutter threaded set -ZERO pixel set)

$1,814

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-378/52-25-STR	$375
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-474/27-25-STR	$375
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF03-575/25-25-STR	$345
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
-ZERO set certification		-ZERO	$99

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:

These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.

Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LED-DA/FI/TX-3X-B-NQF (set mounted in cube)

$2,050

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
Quadfluor cube		NQF	$395

LED-DA/FI/TX-3X-B-NQF-ZERO (-ZERO pixel set mounted in cube)

$2,149

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
Quadfluor cube		NQF	$395
-ZERO set certification		-ZERO	$99

Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LED-DA/FI/TX-3X-B-NTE (set mounted in cube)

$2,050

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
TE 2000 cube		NTE	$395

LED-DA/FI/TX-3X-B-NTE-ZERO (-ZERO pixel set mounted in cube)

$2,149

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
TE 2000 cube		NTE	$395
-ZERO set certification		-ZERO	$99

Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LED-DA/FI/TX-3X-B-OMF (set mounted in cube)

$2,090

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
U-MF2 cube		OMF	$435

LED-DA/FI/TX-3X-B-OMF-ZERO (-ZERO pixel set mounted in cube)

$2,189

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
U-MF2 cube		OMF	$435
-ZERO set certification		-ZERO	$99

Olympus U-FF for microscopes: BX53, BX63

LED-DA/FI/TX-3X-B-OFF (set mounted in cube)

$2,090

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
U-FF cube		OFF	$435

LED-DA/FI/TX-3X-B-OFF-ZERO (-ZERO pixel set mounted in cube)

$2,189

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
U-FF cube		OFF	$435
-ZERO set certification		-ZERO	$99

Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LED-DA/FI/TX-3X-B-ZHE (set mounted in cube)

$1,930

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
FL Cube EC P+C cube		ZHE	$275

LED-DA/FI/TX-3X-B-ZHE-ZERO (-ZERO pixel set mounted in cube)

$2,029

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
FL Cube EC P+C cube		ZHE	$275
-ZERO set certification		-ZERO	$99

Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B

LED-DA/FI/TX-3X-B-LDMK-ZERO (-ZERO pixel set mounted in cube)

$2,104

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	23.3 mm x 2.0 mm	FF01-432/523/702-23.3-D	$465
Triple Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF409/493/596-Di02-22x29	$465
DM-K cube		LDMK	$350
-ZERO set certification		-ZERO	$99

Leica DMi8 (P-Cube) for microscopes: DMi8

LED-DA/FI/TX-3X-B-LDMP-ZERO (-ZERO pixel set mounted in cube)

$2,189

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-378/52-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF01-474/27-25	$355
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-432/523/702-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF409/493/596-Di02-25x36	$465
DMi8 (P-Cube) cube		LDMP	$435
-ZERO set certification		-ZERO	$99

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Blue

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
Alexa Fluor^® 405	401	422
BD Horizon V450	406	449
BFP (EBFP)	380	440
Cascade Blue™	401	419
CellTrace calcein violet	400	452
DAPI	359	461
DyLight 405	398	420
HiLyte Fluor™ 405	403	428
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
LysoSensor Blue	374	424
Marina Blue	363	461
Pacific Blue™	404	455
sgBFP™	387	451
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Green

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
5-FAM (5-carboxyfluorescein)	492	518
Acridine Orange (+DNA)	502	526
Acridine Yellow	470	550
Alexa Fluor^® 430	431	541
Alexa Fluor^® 488	499	520
Alexa Fluor^® 500	503	525
AmCyan1	458	489
ATTO 465	453	507
ATTO 488	500	525
ATTO 495	495	527
BOBO™-1	461	484
BODIPY FL	505	512
BODIPY FL-MeOH	502	511
Calcein	494	514
CFP2	490	510
Cy2™	492	507
CyQUANT GR-DNA	502	523
DAF-FM-NO	495	519
Dendra2 (Green)	491	507
DiO	475	500
Dronpa	502	516
DY-505-Phalloidin	503	530
ecliptic pHluorin pH5.5	473	507
Emerald	491	511
evoglow-Bs1	449	496
FITC (Fluorescein)	495	519
Fluo-4	494	516
Fluorescein dextran	501	524
Fluorescein-pH 8.0	489	517
Fluoro-Emerald	494	518
FluoSpheres Yellow-Green fluorescent microspheres	503	514
GFP (EGFP)	489	511
Green 496	496	520
Green 500	501	524
HCS LipidTOX Green phospholipidosis	504	536
HiLyte Fluor™ 488	497	526
LIVE-DEAD Fixable Green Dead Cell Stain	498	525
LysoSensor Green	448	502
LysoTracker Green	501	509
LysoTracker Yellow HCK-123	488	565
mHoneyDew	478	562
MitoTracker™ Green	490	512
mTFP1	462	492
mWasabi	493	509
NBD-X (MeOH)	467	538
NeuroTrace 500/525 Green Fluorescent Nissl Stain	495	524
Oregon Green™ 488	498	526
PicoGreen^®	502	522
PKH67	488	500
Qdot^® 525 Nanocrystals	300	525
Qdot^® 545 Nanocrystals	300	543
Rhodamine 110	497	519
Rhodamine Green	497	523
Rhodol Green	497	524
sgGFP™ (super glow GFP)	472	506
Sodium Green	507	532
SpectrumGreen	497	538
SYBR Gold nucleic acid gel stain-DNA	496	539
SYBR Green I nucleic acid gel stain-DNA	498	522
SYBR Safe DNA gel stain-DNA	509	526
SYTO 11	506	525
SYTO 13	448	506
SYTO 16	489	520
SYTO 9	483	500
SYTO RNASelect geen fluorescent cell stain	503	527
SYTOX Green-DNA	504	524
TagCFP	458	480
TOTO-1	514	531
TurboGFP	482	503
Vybrant DyeCycle Green	506	534
wtGFP (wild type GFP, non-UV excitation)	474	509
YO-PRO-1	491	506
YOYO-1	491	508

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Red

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
2-dodecylresorufin-lipid	582	595
5-carboxynapthofluorescein (pH 10)	555	615
5-ROX (carboxy-X-rhodamine)	578	604
7-AAD (7-aminoactinomycin D)	548	648
Alexa Fluor^® 568	579	603
Alexa Fluor^® 594	590	618
Alexa Fluor^® 660	663	691
AsRed 2	576	592
ATTO 565	563	592
ATTO 590	594	624
ATTO 594	601	624
BOBO™-3	570	605
BODIPY TR-X phallacidin	590	621
Calcium Crimson	589	609
Carboxynaphthofluorescein	599	674
CellTrace BODIPY TR methyl ester	598	625
CellTracker Red CMTPX	586	614
Cy3.5™	578	591
DDAO	648	657
DY-590	582	599
DY-630	634	656
DyLight 594	593	618
FluoSpheres Crimson fluorescent microspheres	620	646
FluoSpheres Red fluorescent microspheres	577	607
FM 4-64	505	725
HcRed1	588	618
HCS LipidTOX Red neutral lipid stain	582	616
HCS LipidTOX Red phospholipidosis	584	608
HiLyte Fluor™ 594	592	616
KFP-Red	575	599
LDS 751	560	711
LIVE-DEAD Fixable Red Dead Cell Stain	595	613
LysoTracker Red	573	592
mApple	566	594
mCherry	587	610
MitoTracker™ Red	578	598
mPlum	589	649
mRFP1	584	700
mRuby	558	605
mStrawberry	574	596
Nile blue-EtOH	631	660
Nile red-phospolipid	553	637
Orange 552	552	574
PerCP	490	677
Propidium Iodide (PI)	305	617
Qdot^® 605 Nanocrystals	300	602
Qdot^® 625 Nanocrystals	300	621
Qdot^® 655 Nanocrystals	300	654
Qdot^® 705 Nanocrystals	300	702
Qdot^® 800 Nanocrystals	300	792
ReAsH-CCPGCC	592	606
Red 580	580	603
Rhodamine Red-X	573	591
SNARF (carboxy) 514 Excitation pH9	576	638
SNARF-1 488nm (ph 9.0)	576	638
SNARF-1 514nm (ph 9.0)	576	638
SpectrumRed	587	615
Sulforhodamine 101-EtOH	578	593
SYTO 17	619	638
SYTO 61	618	650
Texas Red dextran	592	613
Texas Red DHPE	584	608
Texas Red-X^®	595	613
TurboFP635(Katushka)	591	638
X-Rhod-1 Indicator	580	601
YO-PRO-3	613	629
YOYO-3	611	631

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set Terminology	Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets

Journal Article

Topic	Description
New Optical Filters Improve High-Speed Multicolor Fluorescence Imaging	Turan Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006 Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging

White Paper

Topic	Description
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.

LED-DA/FI/TX-3X-B DA/FI/TX DA/FI/TXRED DAPI/FITC/TexasRed DAPI FITC TexasRed TxRed Pinkel LED 392/23 474/27 575/25 432/523/702 409/493/596 Triple-Band FF01-392/23 FF01-432/523/702 FF01-474/27 FF409/493/596-Di01 FF03-575/25 FF409/493/596 Lumencor Sola Spectra-X CoolLED pE-4000 pE-300-White X-Cite Turbo XLED1 110LED-Broadband 120LED-Broadband Alexa-Fluor-405 Alexa405 AF405 BFP Alexa488 AF488 GFP TurboGFP ROX 5-ROX Alexa568 AF568 Alexa594 AF594 mCherry mPlum mRFP1 Filter Filters Set Sets Triple 3X LED-DA/FI/TX-3X-B-000 LED-DA/FI/TX-3X-B-000-ZERO LED-DA/FI/TX-3X-B-L01-ZERO LED-DA/FI/TX-3X-B-S01 LED-DA/FI/TX-3X-B-S01-ZERO LED-DA/FI/TX-3X-B-NQF LED-DA/FI/TX-3X-B-NQF-ZERO LED-DA/FI/TX-3X-B-NTE LED-DA/FI/TX-3X-B-NTE-ZERO LED-DA/FI/TX-3X-B-OMF LED-DA/FI/TX-3X-B-OMF-ZERO LED-DA/FI/TX-3X-B-OFF LED-DA/FI/TX-3X-B-OFF-ZERO LED-DA/FI/TX-3X-B-ZHE LED-DA/FI/TX-3X-B-ZHE-ZERO LED-DA/FI/TX-3X-B-LDMP-ZERO LED-DA/FI/TX-3X-B-LDMK-ZERO

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores, illuminated with LED-based light engines

Part Number: LED-DA/FI/TX-3X-B-000

BrightLine® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores, illuminated with LED-based light engines

BrightLine^® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores, illuminated with LED-based light engines

BrightLine^® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores, illuminated with LED-based light engines