Welcome, Guest: Login or Register My Cart (0)

BrightLine® Sedat filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines

Semrock Set / Fluorophore Graphing

Part Number: LED-DA/FI/TR/Cy5-4X4M-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: LED-DA/FI/TR/Cy5-4X4M-B

BrightLine® Sedat filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 9-filter "Sedat" quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter with four single-band emitters intended to be mounted in filter wheels and four single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight spectral plotting & analysis tool.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-000 (Standard size set, fits most microscopes2)$2,565Ships in 2 daysAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-378/52-25$355
Single Band Exciter25 mm x 5.0 mmFF01-474/27-25$355
Single Band Exciter25 mm x 5.0 mmFF01-554/23-25$305
Single Band Exciter25 mm x 5.0 mmFF01-635/18-25$305
Single Band Emitter25 mm x 3.5 mmFF01-432/36-25$355
Single Band Emitter25 mm x 3.5 mmFF01-515/30-25$355
Single Band Emitter25 mm x 3.5 mmFF01-595/31-25$355
Single Band Emitter25 mm x 3.5 mmFF01-698/70-25$355
Quad Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF409/493/573/652-Di02-25x36$565
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-L01 (Leica 'Large' set)$2,565Ships in 2 daysAdd To Cart
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-S01 (Sutter threaded set)$2,725Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-NQF (set mounted in cube)$2,960Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-NTE (set mounted in cube)$2,960Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-OMF (set mounted in cube)$3,000Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-OFF (set mounted in cube)$3,000Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-ZHE (set mounted in cube)$2,840Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-LDMK (set mounted in cube)$2,915Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LED-DA/FI/TR/Cy5-4X4M-B-LDMP (set mounted in cube)$3,000Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, and Zeiss microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724771-Star
7-amino-4-methylcoumarin (AMC)3464423-Star
7-hydroxy-4-methylcoumarin3604425-Star
Alexa Fluor® 3503434413-Star
Alexa Fluor® 4054014225-Star
AMCA (Aminomethylcoumarin)3504485-Star
BD Horizon V4504064495-Star
BFP (EBFP)3804405-Star
BUV395 - BD Horizon Brilliant™ Ultraviolet 3953483951-Star
Calcofluor White3514445-Star
Cascade Blue™4014195-Star
CellTrace calcein violet4004523-Star
DAPI3594615-Star
DiFMU-pH 9.03584503-Star
DyLight 3503514345-Star
DyLight 4053984205-Star
Fluoro-Gold™3654183-Star
FluoSpheres Blue fluorescent microspheres3564145-Star
HiLyte Fluor™ 4054034285-Star
Hoechst 332583524553-Star
LIVE-DEAD Fixable Blue Dead Cell Stain3434413-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034551-Star
LysoSensor Blue3744245-Star
LysoTracker Blue3734225-Star
Marina Blue3634613-Star
Pacific Blue™4044551-Star
Quinine sulfate3494613-Star
sgBFP™3874515-Star
SpectrumBlue4054493-Star
TagBFP4024571-Star
Vybrant DyeCycle Violet3694375-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-FAM (5-carboxyfluorescein)4925185-Star
Acridine Orange (+DNA)5025263-Star
Acridine Yellow4705503-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995205-Star
Alexa Fluor® 5005035253-Star
AmCyan14584895-Star
ATTO 4654535071-Star
ATTO 4885005255-Star
ATTO 4954955275-Star
BOBO™-14614843-Star
BODIPY FL5055125-Star
BODIPY FL-MeOH5025115-Star
Calcein4945145-Star
CFP24905105-Star
Cy2™4925075-Star
CyQUANT GR-DNA5025235-Star
DAF-FM-NO4955195-Star
Dendra2 (Green)4915075-Star
DiO4755005-Star
Dronpa5025165-Star
DY-505-Phalloidin5035303-Star
ecliptic pHluorin pH5.54735075-Star
Emerald4915115-Star
evoglow-Bs14494965-Star
FITC (Fluorescein)4955195-Star
Fluo-44945165-Star
Fluorescein dextran5015245-Star
Fluorescein-pH 8.04895175-Star
Fluoro-Emerald4945185-Star
FluoSpheres Yellow-Green fluorescent microspheres5035145-Star
GFP (EGFP)4895115-Star
Green 4964965205-Star
Green 5005015245-Star
HiLyte Fluor™ 4884975265-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985255-Star
LysoSensor Green4485025-Star
LysoTracker Green5015095-Star
LysoTracker Yellow HCK-1234885651-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905125-Star
mTFP14624923-Star
mWasabi4935095-Star
NBD-X (MeOH)4675385-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955245-Star
Oregon Green™ 4884985265-Star
PicoGreen®5025225-Star
PKH674885005-Star
Qdot® 525 Nanocrystals3005251-Star
ratiometric pHluorin pH55005095-Star
Rhodamine 1104975195-Star
Rhodamine Green4975235-Star
Rhodol Green4975245-Star
sgGFP™ (super glow GFP)4725065-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965391-Star
SYBR Green I nucleic acid gel stain-DNA4985225-Star
SYBR Safe DNA gel stain-DNA5095263-Star
SYTO 115065253-Star
SYTO 134485063-Star
SYTO 164895205-Star
SYTO 94835005-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TagCFP4584801-Star
TOTO-15145311-Star
TurboGFP4825035-Star
wtGFP (wild type GFP, non-UV excitation)4745095-Star
YO-PRO-14915065-Star
YOYO-14915085-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Orange
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825955-Star
5-ROX (carboxy-X-rhodamine)5786045-Star
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425685-Star
Alexa Fluor® 5465565725-Star
Alexa Fluor® 5555535653-Star
Alexa Fluor® 5685796035-Star
Amplex UltraRed peroxidation product (pH 7.5)5685815-Star
AsRed 25765925-Star
ATTO 5505535765-Star
ATTO 5655635925-Star
BOBO™-35706055-Star
BODIPY TMR-X5445733-Star
BODIPY TR-X (MeOH)5445733-Star
Calcium Crimson5896093-Star
Calcium Orange5896095-Star
CellTracker Red CMTPX5866143-Star
Cy3.5™5785915-Star
Cy3™5545663-Star
Dendra2 (Red)5545695-Star
DiI5515693-Star
DsRed5595835-Star
DsRed-Express5565845-Star
dTomato5565825-Star
DY-5905825993-Star
DyLight 5495535693-Star
Ethidium bromide5186033-Star
Ethidium homodimer5276173-Star
FluoSpheres Red fluorescent microspheres5776075-Star
Gold 5505515643-Star
HCS LipidTOX Red neutral lipid stain5826161-Star
HCS LipidTOX Red phospholipidosis5846083-Star
HiLyte Fluor™ 5555505663-Star
KFP-Red5755995-Star
LOLO-15685805-Star
LysoTracker Red5735925-Star
Magnesium Orange5505745-Star
mApple5665945-Star
mCherry5876103-Star
Merocyanine 5405595795-Star
MitoTracker™ Orange5515755-Star
MitoTracker™ Red5785985-Star
mOrange5465623-Star
mRFP5555835-Star
mRFP15847003-Star
mRuby5586055-Star
mStrawberry5745965-Star
mTangerine5685855-Star
Nile red-triglyceride5105831-Star
OFP5475675-Star
pHrodo™, succinimidyl ester5605875-Star
POPO-35335743-Star
PO-PRO-35395703-Star
Propidium Iodide (PI)3056173-Star
Pro-Q Diamond5565835-Star
Qdot® 585 Nanocrystals3005883-Star
Qdot® 605 Nanocrystals3006023-Star
ReAsH-CCPGCC5926063-Star
Red 5805806033-Star
Resorufin5715845-Star
Rhod-25535775-Star
Rhodamine phalloidin5585755-Star
Rhodamine Red-X5735915-Star
R-phycoerythrin (PE)5655763-Star
SNARF-1 488nm (ph 6.0)5485865-Star
SNARF-1 514nm (ph 6.0)5495875-Star
SpectrumOrange5545875-Star
Sulforhodamine 101-EtOH5785933-Star
SYTO 825405591-Star
SYTOX Orange5475703-Star
Tetramethylrhodamine dextran5545825-Star
Texas Red DHPE5846083-Star
TRITC (Tetramethylrhodamine)5525785-Star
TurboRFP5535735-Star
X-Rhod-1 Indicator5806015-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556153-Star
Alexa Fluor® 6336316473-Star
Alexa Fluor® 6476536685-Star
Alexa Fluor® 6606636915-Star
Alexa Fluor® 6806797025-Star
Alexa Fluor® 7006967193-Star
APC6516605-Star
APC (Allophycocyanin)6516605-Star
ATTO 6336296585-Star
ATTO 6356356585-Star
ATTO 6476446705-Star
ATTO 647N6456685-Star
ATTO 6556636835-Star
ATTO 6806807003-Star
ATTO 7006997193-Star
BODIPY 650/665-X6466645-Star
Carboxynaphthofluorescein5996743-Star
Cy5.5™6726905-Star
Cy5™6496665-Star
DDAO6486575-Star
DiD (DiIC18(5)) - Lipophilic Tracer6486705-Star
DY-6306346565-Star
DY-6756756993-Star
DyLight 6496516735-Star
DyLight 6806767053-Star
FluoSpheres Dark Red fluorescent microspheres6566835-Star
HCS CellMask Red cytoplasmic6226443-Star
HCS LipidTOX Deep Red neutral lipid stain6356523-Star
HiLyte Fluor™ 6476496735-Star
IRDye 680 NHS Ester6837103-Star
IRDye 700Phosphoramidite6916993-Star
LDS 7515607113-Star
LIVE-DEAD Fixable Far Red Dead Cell Stain6536685-Star
MitoTracker™ Deep Red6416625-Star
Nile blue-EtOH6316605-Star
Qdot® 705 Nanocrystals3007021-Star
Quasar® 7056887063-Star
Red 6506496655-Star
SpectrumFRed (Far Red)6506735-Star
SYTO 596226443-Star
SYTO 606506815-Star
SYTO 616186503-Star
SYTOX Red-DNA6406585-Star
TO-PRO-36426575-Star
TOTO-36426615-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
Alternate Quad-Band Filter Set Configurations

LED-DA_FI_TR_Cy5-B.png

BrightLine® full-multiband filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines

Multiband set with quad-band exciter, emitter, and beamsplitter filters. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Orange: TRITC ; Red: Cy5
Part # LED-DA/FI/TR/Cy5-B-000
$1375
(Non-US pricing info)



LED-DA_FI_TR_Cy5-4X-B.png

BrightLine® Pinkel filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines

6-filter "Pinkel" quad-band set with 4 exciters and quad-band emitter and beamsplitter filters. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Orange: TRITC ; Red: Cy5
Part # LED-DA/FI/TR/Cy5-4X-B-000
$2045
(Non-US pricing info)



LED-DA_FI_TR_Cy5_Cy7-5X-A.png

BrightLine® Pinkel filter set, optimized for DAPI, FITC, TRITC, Cy5 & Cy7 and other like fluorophores, illuminated with LED-based light sources

7-filter "Pinkel" penta-band set with 5 exciters, a penta-band emitter and a penta-band dichroic beamsplitter. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Orange: TRITC ; Red: Cy5; Red/ Near-IR: Cy7
Part # LED-DA/FI/TR/Cy5/Cy7-5X-A-000
$2385
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



LED-DA/FI/TR/Cy5-4X4M-B LED 4X4M DAPI FITC TRITC Cy5 DAPI/FITC/TRITC/Cy5 DA/FI/TR/Cy5 Sedat 378/52 FF01-378/52 474/27 FF01-474/27 554/23 FF01-554/23 635/18 FF01-635/18 432/36 FF01-432/36 515/30 FF01-515/30 595/31 FF01-595/31 698/70 FF01-698/70 409/493/573/652 FF409/493/573/652 FF409/493/573/652-Di02 Quad-Band Quad Lumencor Sola Sola365 Spectra-X CoolLED pE-4000 pE-300-White X-Cite Turbo XLED1 110LED-Broadband 120LED-Broadband Filter Filters Set Sets LED-DA/FI/TR/Cy5-4X4M-B-000 LED-DA/FI/TR/Cy5-4X4M-B-L01 LED-DA/FI/TR/Cy5-4X4M-B-S01 LED-DA/FI/TR/Cy5-4X4M-B-NQF LED-DA/FI/TR/Cy5-4X4M-B-NTE LED-DA/FI/TR/Cy5-4X4M-B-OMF LED-DA/FI/TR/Cy5-4X4M-B-OFF LED-DA/FI/TR/Cy5-4X4M-B-ZHE LED-DA/FI/TR/Cy5-4X4M-B-LDMP LED-DA/FI/TR/Cy5-4X4M-B-LDMK

The item has been added to your shopping cart.

The item has been added to your shopping cart, however, your IP address appears to be outside the US/ Canada, so online/ direct orders from Semrock are not allowed. Please see the International Distributor page to find your non-US distributor.

We apologize for any inconvenience.

The shopping cart is offline. Please call toll-free 1-866-SEMROCK (866-736-7625) or email us to place your order.