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BrightLine® single-band filter set, optimized for DAPI and other like fluorophores, illuminated with 365 to 395 nm LED-based light engines

Semrock Set / Fluorophore Graphing

Part Number: LED-DAPI-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: LED-DAPI-B

BrightLine® single-band filter set, optimized for DAPI and other like fluorophores, illuminated with 365 to 395 nm LED-based light engines

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring DAPI (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market. Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LED-DAPI-B-000 (Standard size set, fits most microscopes2)$785In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-378/52-25$355
Single Band Emitter25 mm x 3.5 mmFF02-447/60-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF409-Di03-25x36$255
Click to expand/collapse details LED-DAPI-B-000-ZERO (Standard size -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details LED-DAPI-B-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details LED-DAPI-B-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LED-DAPI-B-NQF (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details LED-DAPI-B-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LED-DAPI-B-NTE (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details LED-DAPI-B-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LED-DAPI-B-OMF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details LED-DAPI-B-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LED-DAPI-B-OFF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details LED-DAPI-B-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details LED-DAPI-B-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LED-DAPI-B-ZHE (set mounted in cube)$1,060In StockAdd To Cart
Click to expand/collapse details LED-DAPI-B-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LED-DAPI-B-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LED-DAPI-B-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724771-Star
Alexa Fluor® 4054014225-Star
Aqua 4314304781-Star
ATTO 3903924773-Star
BD Horizon V4504064495-Star
BFP (EBFP)3804405-Star
Cascade Blue™4014195-Star
CellTrace calcein violet4004525-Star
CFP (ECFP)4334751-Star
DAPI3594615-Star
DY-4154184701-Star
DyLight 4053984205-Star
ECFP4354751-Star
HiLyte Fluor™ 4054034285-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034555-Star
LysoSensor Blue3744245-Star
Marina Blue3634611-Star
mBBr+GSH3944901-Star
Pacific Blue™4044555-Star
sgBFP™3874515-Star
SpectrumBlue4054495-Star
TagBFP4024575-Star
Vybrant DyeCycle Violet3694373-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



LED-DAPI-A LED-DAPI-B DAPI LED 378/52 409 447/60 Single-Band FF409-Di03 FF01-378/52 FF02-447/60 FF409 FF409-Di03-25x36 FF01-378/52-25 FF02-447/60-25 Lumencor Sola Sola365 Spectra-X CoolLED pE-4000 pE-300-White X-Cite Turbo XLED1 110LED-Broadband 120LED-Broadband Alexa-Fluor-405 Alexa405 AF405 BFP CascadeBlue DyLight405 HiLyte405 PacificBlue TagBFP SpectrumBlue sgBFP LysoSensorBlue Filter Filters Set Sets LED-DAPI-B-000 LED-DAPI-B-000-ZERO LED-DAPI-B-L01-ZERO LED-DAPI-B-U02 LED-DAPI-B-NQF LED-DAPI-B-NQF-ZERO LED-DAPI-B-NTE LED-DAPI-B-NTE-ZERO LED-DAPI-B-OMF LED-DAPI-B-OMF-ZERO LED-DAPI-B-OFF LED-DAPI-B-OFF-ZERO LED-DAPI-B-OFX LED-DAPI-B-ZHE LED-DAPI-B-ZHE-ZERO LED-DAPI-B-LDMP-ZERO LED-DAPI-B-LDMK-ZERO LED-DAPI-B-AMF-ZERO

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