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BrightLine^® single-band filter set, optimized for Cy7 and other like fluorophores, illuminated with LED-based light sources

Semrock Set / Fluorophore Graphing

Part Number: LED-Cy7-A-000

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Filters and Pricing
Specifications
Fluorophore
Technical Info

BrightLine^® single-band filter set, optimized for Cy7 and other like fluorophores, illuminated with LED-based light sources

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Venus (or other key fluorophores – see complete list above). This state-of-the-art set has been optimized for popular LED based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

Part Number

Price¹

Stock Status

Quantity

LED-Cy7-A-000 (Standard size set, fits most microscopes²)

$825

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275

LED-Cy7-A-000-ZERO (Standard size -ZERO pixel set)

$924

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
-ZERO set certification		-ZERO	$99

LED-Cy7-A-L01-ZERO (Leica 'Large' -ZERO pixel set)

$924

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	21.8 mm x 2.0 mm	FF01-735/28-21.8-D	$355
Single Band Emitter	23.3 mm x 2.0 mm	FF02-809/81-23.3-D	$325
Single Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF757-Di01-22x29	$275
-ZERO set certification		-ZERO	$99

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	32 mm x 5.0 mm	FF01-735/28-32	$582

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:

These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.

Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LED-Cy7-A-NQF (set mounted in cube)

$1,220

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
Quadfluor cube		NQF	$395

LED-Cy7-A-NQF-ZERO (-ZERO pixel set mounted in cube)

$1,319

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
Quadfluor cube		NQF	$395
-ZERO set certification		-ZERO	$99

Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LED-Cy7-A-NTE (set mounted in cube)

$1,220

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
TE 2000 cube		NTE	$395

LED-Cy7-A-NTE-ZERO (-ZERO pixel set mounted in cube)

$1,319

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
TE 2000 cube		NTE	$395
-ZERO set certification		-ZERO	$99

Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LED-Cy7-A-OMF (set mounted in cube)

$1,260

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
U-MF2 cube		OMF	$435

LED-Cy7-A-OMF-ZERO (-ZERO pixel set mounted in cube)

$1,359

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
U-MF2 cube		OMF	$435
-ZERO set certification		-ZERO	$99

Olympus U-FF for microscopes: BX53, BX63

LED-Cy7-A-OFF (set mounted in cube)

$1,260

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
U-FF cube		OFF	$435

LED-Cy7-A-OFF-ZERO (-ZERO pixel set mounted in cube)

$1,359

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
U-FF cube		OFF	$435
-ZERO set certification		-ZERO	$99

Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LED-Cy7-A-ZHE (set mounted in cube)

$1,100

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
FL Cube EC P+C cube		ZHE	$275

LED-Cy7-A-ZHE-ZERO (-ZERO pixel set mounted in cube)

$1,199

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
FL Cube EC P+C cube		ZHE	$275
-ZERO set certification		-ZERO	$99

Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B

LED-Cy7-A-LDMK-ZERO (-ZERO pixel set mounted in cube)

$1,274

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	21.8 mm x 2.0 mm	FF01-735/28-21.8-D	$355
Single Band Emitter	23.3 mm x 2.0 mm	FF02-809/81-23.3-D	$325
Single Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF757-Di01-22x29	$275
DM-K cube		LDMK	$350
-ZERO set certification		-ZERO	$99

Leica DMi8 (P-Cube) for microscopes: DMi8

LED-Cy7-A-LDMP-ZERO (-ZERO pixel set mounted in cube)

$1,359

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-735/28-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF02-809/81-25	$325
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF757-Di01-25x36	$275
DMi8 (P-Cube) cube		LDMP	$435
-ZERO set certification		-ZERO	$99

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
Alexa Fluor^® 750	751	776
Alexa Fluor^® 790	782	805
ATTO 725	727	754
ATTO 740	740	764
Cy7™	753	775
DiR	750	782
DY-781	784	802
DyLight 750	751	778
DyLight 800	777	794
HiLyte Fluor™ 750	750	778
IRDye 800 CW-MeOH	778	801
IRDye 800CW	775	792
IRDye 800CW-NHS Ester	775	792
IRDye 800RS	787	812
LIVE-DEAD Fixable Near-IR Dead Cell Stain	752	776

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?	Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity

Journal Article

Topic	Description
Achieving the Best Alignment for Fluorescent Images	Turan Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005 Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image

White Paper

Topic	Description
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.

LED-Cy7-A YFP Cy7 LED 735/28 809/81 757 Single-Band FF757-Di01 FF01-735/28 FF02-809/81 FF757-Di01-25x36 FF01-735/28-25 FF02-809/81-25 FF757 Lumencor Spectra-X-nIR CoolLED pE-4000 X-Cite XLED1 Filter Filters Set Sets Alexa-Fluor-750 Alexa750 AF750 ATTO740 DyLight750 HiLyteFluor750 DiR LED-Based-Light-Engine LED-Cy7-A-000 LED-Cy7-A-000-ZERO LED-Cy7-A-L01-ZERO LED-Cy7-A-NQF LED-Cy7-A-NQF-ZERO LED-Cy7-A-NTE LED-Cy7-A-NTE-ZERO LED-Cy7-A-OMF LED-Cy7-A-OMF-ZERO LED-Cy7-A-OFF LED-Cy7-A-OFF-ZERO LED-Cy7-A-ZHE LED-Cy7-A-ZHE-ZERO LED-Cy7-A-LDMP-ZERO LED-Cy7-A-LDMK-ZERO

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® single-band filter set, optimized for Cy7 and other like fluorophores, illuminated with LED-based light sources

Part Number: LED-Cy7-A-000

BrightLine® single-band filter set, optimized for Cy7 and other like fluorophores, illuminated with LED-based light sources

BrightLine^® single-band filter set, optimized for Cy7 and other like fluorophores, illuminated with LED-based light sources

BrightLine^® single-band filter set, optimized for Cy7 and other like fluorophores, illuminated with LED-based light sources