Welcome, Guest: Login or Register My Cart (0)
SetDetails

BrightLine® single-band filter set, optimized for mTFP (Teal) and other like fluorophores, illuminated with LED-based light sources

Semrock Set / Fluorophore Graphing

Part Number: LED-mTFP-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: LED-mTFP-A

BrightLine® single-band filter set, optimized for mTFP (Teal) and other like fluorophores, illuminated with LED-based light sources

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring mTFP (Teal) (or other key fluorophores – see complete list above). This state-of-the-art set has been optimized for popular LED based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LED-mTFP-A-000 (Standard size set, fits most microscopes2)$825Confirmed AROAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-442/42-25$355
Single Band Emitter25 mm x 3.5 mmFF01-494/34-25$355
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF470-Di01-25x36$275
Click to expand/collapse details LED-mTFP-A-000-ZERO (Standard size -ZERO pixel set)$924Confirmed AROAdd To Cart
Click to expand/collapse details LED-mTFP-A-L01-ZERO (Leica 'Large' -ZERO pixel set)$924Confirmed AROAdd To Cart
Click to expand/collapse details LED-mTFP-A-U02 (Olympus large-size (FX) set for U-M710I)$1,370Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LED-mTFP-A-NQF (set mounted in cube)$1,220Confirmed AROAdd To Cart
Click to expand/collapse details LED-mTFP-A-NQF-ZERO (-ZERO pixel set mounted in cube)$1,319Confirmed AROAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LED-mTFP-A-NTE (set mounted in cube)$1,220Confirmed AROAdd To Cart
Click to expand/collapse details LED-mTFP-A-NTE-ZERO (-ZERO pixel set mounted in cube)$1,319Confirmed AROAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LED-mTFP-A-OMF (set mounted in cube)$1,260Confirmed AROAdd To Cart
Click to expand/collapse details LED-mTFP-A-OMF-ZERO (-ZERO pixel set mounted in cube)$1,359Confirmed AROAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LED-mTFP-A-OFF (set mounted in cube)$1,260Confirmed AROAdd To Cart
Click to expand/collapse details LED-mTFP-A-OFF-ZERO (-ZERO pixel set mounted in cube)$1,359Confirmed AROAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details LED-mTFP-A-OFX (set mounted in cube)$2,065Confirmed AROAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LED-mTFP-A-ZHE (set mounted in cube)$1,100Confirmed AROAdd To Cart
Click to expand/collapse details LED-mTFP-A-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,199Confirmed AROAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LED-mTFP-A-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,274Confirmed AROAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LED-mTFP-A-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,359Confirmed AROAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Acridine Yellow4705501-Star
AmCyan14584895-Star
Aqua 4314304785-Star
ATTO 4254364845-Star
ATTO 4654535071-Star
BD Horizon V5004105001-Star
BOBO™-14614843-Star
Cerulean4344735-Star
CFP (ECFP)4334755-Star
DY-4154184701-Star
ECFP4354755-Star
evoglow-Bs14494963-Star
LysoSensor Green4485021-Star
mBBr+GSH3944901-Star
mTFP14624925-Star
POPO-14334571-Star
PO-PRO-14334561-Star
SpectrumAqua4344815-Star
SYTO 454514855-Star
SYTOX Blue4444705-Star
TagCFP4584805-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



LED-mTFP-A CFP mTFP mTFP1 Teal LED 442/42 494/34 470 Single-Band FF470-Di01 FF01-442/42 FF01-494/34 FF470-Di01-25x36 FF01-494/34-25 FF01-442/42-25 FF470 Lumencor Sola Spectra-X CoolLED pE-4000 X-Cite Turbo XLED1 120LED-Broadband pE-300-White LED-mTFP-A-000 LED-mTFP-A-000-ZERO LED-mTFP-A-L01-ZERO LED-mTFP-A-U02 LED-mTFP-A-NQF LED-mTFP-A-NQF-ZERO LED-mTFP-A-NTE LED-mTFP-A-NTE-ZERO LED-mTFP-A-OMF LED-mTFP-A-OMF-ZERO LED-mTFP-A-OFF LED-mTFP-A-OFF-ZERO LED-mTFP-A-OFX LED-mTFP-A-ZHE LED-mTFP-A-ZHE-ZERO LED-mTFP-A-LDMP-ZERO LED-mTFP-A-LDMK-ZERO

The item has been added to your shopping cart.

The item has been added to your shopping cart, however, your IP address appears to be outside the US/ Canada, so online/ direct orders from Semrock are not allowed. Please see the International Distributor page to find your non-US distributor.

We apologize for any inconvenience.

The shopping cart is offline. Please call toll-free 1-866-SEMROCK (866-736-7625) or email us to place your order.