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BrightLine® Pinkel filter set, optimized for DAPI, FITC, TRITC, Cy5 & Cy7 and other like fluorophores, illuminated with LED-based light sources

Semrock Set / Fluorophore Graphing

Part Number: LED-DA/FI/TR/Cy5/Cy7-5X-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: LED-DA/FI/TR/Cy5/Cy7-5X-A

BrightLine® Pinkel filter set, optimized for DAPI, FITC, TRITC, Cy5 & Cy7 and other like fluorophores, illuminated with LED-based light sources

This 7-filter "Pinkel" penta-band set is designed for imaging of a sample that is simultaneously labeled with DAPI, FITC, TRITC, Cy5, and Cy7 (or other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized for popular LED-based light sources. This set has been designed to deliver significant fluorescence signal improvements for LED-based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a penta-band beamsplitter and a penta-band emitter (these can be offered assembled in a microscope cube), plus five "no burn-out" single-band exciters that are intended to be mounted in the LED based light source, or in an exciter filter wheel or slider.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-000 (Standard size set, fits most microscopes2)$2,685Confirmed AROAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-378/52-25$355
Single Band Exciter25 mm x 5.0 mmFF01-474/27-25$355
Single Band Exciter25 mm x 5.0 mmFF01-554/23-25$325
Single Band Exciter25 mm x 5.0 mmFF01-635/18-25$325
Single Band Exciter25 mm x 5.0 mmFF01-735/28-25$355
Penta Band Emitter25 mm x 3.5 mmFF01-432/515/595/681/809-25$665
Penta Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF409/493/573/652/759-Di01-25x36$665
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-000-ZERO (Standard size -ZERO pixel set)$2,784Confirmed AROAdd To Cart
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-L01-ZERO (Leica 'Large' -ZERO pixel set)$2,784Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-NQF (set mounted in cube)$3,080Confirmed AROAdd To Cart
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-NQF-ZERO (-ZERO pixel set mounted in cube)$3,179Confirmed AROAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-NTE (set mounted in cube)$3,080Confirmed AROAdd To Cart
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-NTE-ZERO (-ZERO pixel set mounted in cube)$3,179Confirmed AROAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-OMF (set mounted in cube)$3,120Confirmed AROAdd To Cart
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-OMF-ZERO (-ZERO pixel set mounted in cube)$3,219Confirmed AROAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-OFF (set mounted in cube)$3,120Confirmed AROAdd To Cart
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-OFF-ZERO (-ZERO pixel set mounted in cube)$3,219Confirmed AROAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-ZHE (set mounted in cube)$2,960Confirmed AROAdd To Cart
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-ZHE-ZERO (-ZERO pixel set mounted in cube)$3,059Confirmed AROAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-LDMK-ZERO (-ZERO pixel set mounted in cube)$3,134Confirmed AROAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LED-DA/FI/TR/Cy5/Cy7-5X-A-LDMP-ZERO (-ZERO pixel set mounted in cube)$3,219Confirmed AROAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724771-Star
4-methylumbelliferone3574411-Star
7-hydroxy-4-methylcoumarin3604423-Star
Alexa Fluor® 4054014225-Star
AMCA (Aminomethylcoumarin)3504483-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804405-Star
BV421 - BD Horizon Brilliant™ Violet 4214074215-Star
Calcofluor White3514445-Star
Cascade Blue™4014195-Star
CellTrace calcein violet4004523-Star
DAPI3594615-Star
DiFMU-pH 9.03584503-Star
DyLight 4053984205-Star
HiLyte Fluor™ 4054034285-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
LysoSensor Blue3744245-Star
LysoTracker Blue3734223-Star
Marina Blue3634611-Star
Pacific Blue™4044553-Star
sgBFP™3874515-Star
Sirius3554241-Star
SpectrumBlue4054493-Star
TagBFP4024571-Star
Vybrant DyeCycle Violet3694375-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005251-Star
5-FAM (5-carboxyfluorescein)4925185-Star
Acridine Orange (+DNA)5025261-Star
Alexa Fluor® 4884995203-Star
Alexa Fluor® 5005035251-Star
AmCyan14584891-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025283-Star
BODIPY FL5055121-Star
BODIPY FL-MeOH5025111-Star
Calcein4945143-Star
Calcium Green-15075291-Star
CFP24905105-Star
Cy2™4925075-Star
CyQUANT GR-DNA5025231-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915073-Star
DiO4755003-Star
Dronpa5025161-Star
DY-505-Phalloidin5035301-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915115-Star
FITC (Fluorescein)4955195-Star
FlAsH-CCPFCC5115301-Star
Fluo-35065271-Star
Fluo-44945163-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895173-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
GFP (EGFP)4895115-Star
Green 4964965203-Star
Green 5005015243-Star
HCS LipidTOX Green neutral lipid stain4985073-Star
HCS LipidTOX Green phospholipidosis5045361-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoSensor Green4485021-Star
LysoTracker Green5015093-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075311-Star
mHoneyDew4785623-Star
MitoTracker™ Green4905123-Star
mWasabi4935095-Star
NBD-X (MeOH)4675381-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135321-Star
PicoGreen®5025221-Star
PKH674885001-Star
Qdot® 525 Nanocrystals3005251-Star
ratiometric pHluorin pH55005093-Star
Rhodamine 1104975193-Star
Rhodamine 1235075291-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
sgGFP™ (super glow GFP)4725065-Star
Sodium Green5075321-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485065-Star
SYTO 164895203-Star
SYTO 94835001-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboGFP4825035-Star
Vybrant DyeCycle Green5065341-Star
wtGFP (wild type GFP, non-UV excitation)4745093-Star
YFP (yellow GFP)5135301-Star
YO-PRO-14915063-Star
YOYO-14915085-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Orange
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825955-Star
5-ROX (carboxy-X-rhodamine)5786045-Star
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425681-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5465565725-Star
Alexa Fluor® 5555535653-Star
Alexa Fluor® 5685796033-Star
Alexa Fluor® 5945906181-Star
Amplex UltraRed peroxidation product (pH 7.5)5685815-Star
AsRed 25765923-Star
ATTO 5505535765-Star
ATTO 5655635925-Star
ATTO 5905946241-Star
BOBO™-35706053-Star
BODIPY TMR-X5445731-Star
BODIPY TR-X (MeOH)5445733-Star
BODIPY TR-X phallacidin5906211-Star
Calcium Crimson5896091-Star
Calcium Orange5896093-Star
CellTrace BODIPY TR methyl ester5986251-Star
CellTracker Red CMTPX5866141-Star
Cy3.5™5785915-Star
Dendra2 (Red)5545695-Star
DiI5515693-Star
DsRed5595835-Star
DsRed-Express5565845-Star
dTomato5565825-Star
DY-5905825993-Star
DyLight 5945936181-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276171-Star
FluoSpheres Red fluorescent microspheres5776073-Star
HcRed15886181-Star
HCS LipidTOX Red neutral lipid stain5826161-Star
HCS LipidTOX Red phospholipidosis5846083-Star
HiLyte Fluor™ 5945926161-Star
KFP-Red5755995-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956131-Star
LOLO-15685805-Star
LysoTracker Red5735925-Star
Magnesium Orange5505745-Star
mApple5665945-Star
mCherry5876103-Star
Merocyanine 5405595795-Star
MitoTracker™ Orange5515753-Star
MitoTracker™ Red5785983-Star
mKate25886331-Star
mOrange5465623-Star
mOrange25515653-Star
mRFP5555835-Star
mRFP15847003-Star
mRuby5586055-Star
mStrawberry5745965-Star
mTangerine5685855-Star
Nile red-phospolipid5536371-Star
Nile red-triglyceride5105831-Star
OFP5475673-Star
Orange 5525525745-Star
pHrodo™, succinimidyl ester5605875-Star
POPO-35335741-Star
Propidium Iodide (PI)3056173-Star
Pro-Q Diamond5565835-Star
Qdot® 585 Nanocrystals3005883-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
ReAsH-CCPGCC5926061-Star
Red 5805806033-Star
Resorufin5715845-Star
Rhod-25535775-Star
Rhodamine phalloidin5585755-Star
Rhodamine Red-X5735915-Star
SNARF (carboxy) 514 Excitation pH95766381-Star
SNARF-1 488nm (ph 6.0)5485865-Star
SNARF-1 488nm (ph 9.0)5766381-Star
SNARF-1 514nm (ph 6.0)5495873-Star
SNARF-1 514nm (ph 9.0)5766381-Star
SpectrumOrange5545875-Star
Sulforhodamine 101-EtOH5785933-Star
SYTOX Orange5475701-Star
Tetramethylrhodamine dextran5545825-Star
Texas Red dextran5926131-Star
Texas Red DHPE5846083-Star
Texas Red®5926143-Star
TRITC (Tetramethylrhodamine)5525785-Star
TurboFP635(Katushka)5916381-Star
TurboRFP5535735-Star
X-Rhod-1 Indicator5806013-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 6476536685-Star
Alexa Fluor® 6606636915-Star
Alexa Fluor® 6806797021-Star
APC6516605-Star
APC (Allophycocyanin)6516605-Star
ATTO 6356356585-Star
ATTO 6476446705-Star
ATTO 647N6456685-Star
ATTO 6556636835-Star
ATTO 6806807001-Star
BODIPY 650/665-X6466645-Star
Cy5.5™6726903-Star
Cy5™6496665-Star
DDAO6486575-Star
DiD (DiIC18(5)) - Lipophilic Tracer6486705-Star
DY-6306346565-Star
DY-6756756991-Star
DyLight 6496516735-Star
DyLight 6806767051-Star
FluoSpheres Dark Red fluorescent microspheres6566835-Star
HiLyte Fluor™ 6476496735-Star
LIVE-DEAD Fixable Far Red Dead Cell Stain6536685-Star
MitoTracker™ Deep Red6416625-Star
Nile blue-EtOH6316605-Star
Red 6506496655-Star
SpectrumFRed (Far Red)6506735-Star
SYTO 606506815-Star
SYTOX Red-DNA6406585-Star
TO-PRO-36426575-Star
TOTO-36426615-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Deep Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 7507517765-Star
Alexa Fluor® 7907828051-Star
ATTO 7257277543-Star
ATTO 7407407645-Star
Cy7™7537755-Star
DiR7507825-Star
DY-7817848021-Star
DyLight 7507517785-Star
DyLight 8007777941-Star
HiLyte Fluor™ 7507507785-Star
IRDye 800 CW-MeOH7788011-Star
IRDye 800CW7757921-Star
IRDye 800CW-NHS Ester7757921-Star
IRDye 800RS7878121-Star
LIVE-DEAD Fixable Near-IR Dead Cell Stain7527763-Star
Qdot® 800 Nanocrystals3007921-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



LED-DA/FI/TR/Cy5/Cy7-5X-A DA/FI/TR/Cy5/Cy7 DAPI/FITC/TRITC/Cy5/Cy7 DAPI FITC TRITC Cy5 Cy7 Pinkel Penta Penta-Band FF409/493/573/652/759 FF409/493/573/652/759-Di01 FF01-432/515/595/681/809 FF01-378/52 FF01-474/27 FF01-554/23 FF01-635/18 FF01-735/28 409/493/573/652/759 432/515/595/681/809 378/52 474/27 554/23 635/18 735/28 Filter Filters Set Sets Penta Lumencor Sola Spectra-X-nIR Spectra-X nIR CoolLED pE-4000 X-Cite Turbo XLED1 120LED-Broadband 5X LED-DA/FI/TR/Cy5/Cy7-5X-A-000 LED-DA/FI/TR/Cy5/Cy7-5X-A-000-ZERO LED-DA/FI/TR/Cy5/Cy7-5X-A-L01-ZERO LED-DA/FI/TR/Cy5/Cy7-5X-A-NQF LED-DA/FI/TR/Cy5/Cy7-5X-A-NQF-ZERO LED-DA/FI/TR/Cy5/Cy7-5X-A-NTE LED-DA/FI/TR/Cy5/Cy7-5X-A-NTE-ZERO LED-DA/FI/TR/Cy5/Cy7-5X-A-OMF LED-DA/FI/TR/Cy5/Cy7-5X-A-OMF-ZERO LED-DA/FI/TR/Cy5/Cy7-5X-A-OFF LED-DA/FI/TR/Cy5/Cy7-5X-A-OFF-ZERO LED-DA/FI/TR/Cy5/Cy7-5X-A-ZHE LED-DA/FI/TR/Cy5/Cy7-5X-A-ZHE-ZERO LED-DA/FI/TR/Cy5/Cy7-5X-A-LDMP-ZERO LED-DA/FI/TR/Cy5/Cy7-5X-A-LDMK-ZERO

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