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BrightLine^® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 594 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF594/LP-D-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Filters and Pricing
Specifications
Fluorophore
Technical Info

BrightLine^® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 594 nm laser sources

This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from mCherry and other similar fluorophores while offering superb blocking of popular 593.5 nm (Double DPSS), 594 nm (HeNe), and 594.1 nm (DPSS) and 594+/-0.3 nm lasers. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF or LDMK?
Our previous version is still available for those cube configurations LF594/LP-C

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

Part Number

Price¹

Stock Status

Quantity

LF594/LP-D-000 (Standard size set, fits most microscopes²)

$1,035

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-591/6-25	$405
Long Pass Emitter	25 mm x 3.5 mm	BLP01-594R-25	$375
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R594-t1-25x36	$495

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-591/6-25	$405
Long Pass Emitter	25 mm x 3.5 mm	BLP01-594R-25	$375
Single Band Dichroic	25.2 mm x 35.6 mm x 3.0 mm	Di03-R594-t3-25x36	$595

Filter Role

Filter Size

Part Number

Price¹

Filter Role

Filter Size

Part Number

Price¹

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:

These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.

Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LF594/LP-D-NTE (set mounted in cube)

$1,555

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-591/6-25	$405
Long Pass Emitter	25 mm x 3.5 mm	BLP01-594R-25	$375
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R594-t1-25x36	$495
TE 2000 cube		NTE	$395
Super-resolution flatness certification			$125

Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LF594/LP-D-OMF (set mounted in cube)

$1,595

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-591/6-25	$405
Long Pass Emitter	25 mm x 3.5 mm	BLP01-594R-25	$375
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R594-t1-25x36	$495
U-MF2 cube		OMF	$435
Super-resolution flatness certification			$125

Olympus U-FF for microscopes: BX53, BX63

LF594/LP-D-OFF (set mounted in cube)

$1,595

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-591/6-25	$405
Long Pass Emitter	25 mm x 3.5 mm	BLP01-594R-25	$375
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R594-t1-25x36	$495
U-FF cube		OFF	$435
Super-resolution flatness certification			$125

Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LF594/LP-D-ZHE (set mounted in cube)

$1,435

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-591/6-25	$405
Long Pass Emitter	25 mm x 3.5 mm	BLP01-594R-25	$375
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R594-t1-25x36	$495
FL Cube EC P+C cube		ZHE	$275
Super-resolution flatness certification			$125

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
5-carboxynapthofluorescein (pH 10)	555	615
5-ROX (carboxy-X-rhodamine)	578	604
7-AAD (7-aminoactinomycin D)	548	648
Alexa Fluor^® 568	579	603
Alexa Fluor^® 594	590	618
Alexa Fluor^® 660	663	691
APC	651	660
APC (Allophycocyanin)	651	660
AsRed 2	576	592
ATTO 590	594	624
ATTO 594	601	624
ATTO 610	614	634
BOBO™-3	570	605
BODIPY 630/650-X	625	641
BODIPY TR-X phallacidin	590	621
Calcium Crimson	589	609
Carboxynaphthofluorescein	599	674
CellTrace BODIPY TR methyl ester	598	625
CellTracker Red CMTPX	586	614
ChlorophyllA	480	650
DDAO	648	657
DY-590	582	599
DyLight 594	593	618
DyLight 633	620	649
FluoSpheres Crimson fluorescent microspheres	620	646
FluoSpheres Red fluorescent microspheres	577	607
FM 4-64	505	725
HcRed1	588	618
HCS CellMask Red cytoplasmic	622	644
HCS LipidTOX Deep Red neutral lipid stain	635	652
HCS LipidTOX Red neutral lipid stain	582	616
HCS LipidTOX Red phospholipidosis	584	608
HiLyte Fluor™ 594	592	616
KFP-Red	575	599
LDS 751	560	711
LIVE-DEAD Fixable Red Dead Cell Stain	595	613
mCherry	587	610
MitoTracker™ Red	578	598
mKate2	588	633
mPlum	589	649
mRFP1	584	700
Nile blue-EtOH	631	660
Nile red-phospolipid	553	637
Qdot^® 605 Nanocrystals	300	602
Qdot^® 625 Nanocrystals	300	621
Qdot^® 655 Nanocrystals	300	654
Qdot^® 705 Nanocrystals	300	702
Qdot^® 800 Nanocrystals	300	792
ReAsH-CCPGCC	592	606
Red 580	580	603
SNARF (carboxy) 514 Excitation pH9	576	638
SNARF-1 488nm (ph 9.0)	576	638
SNARF-1 514nm (ph 9.0)	576	638
SpectrumRed	587	615
SYTO 17	619	638
SYTO 59	622	644
SYTO 61	618	650
Texas Red dextran	592	613
Texas Red DHPE	584	608
Texas Red^®	592	614
TurboFP635(Katushka)	591	638
X-Rhod-1 Indicator	580	601
YO-PRO-3	613	629
YOYO-3	611	631

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic Beamsplitters	Describes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
Optical Filters for Laser-based Fluorescence Microscopes	Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection

Journal Article

Topic	Description
Bringing Super-resolution to Fluorescence Microscopy	Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010 Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systems	Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF Optics	Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection

White Paper

Topic	Description
Optical Filters for Laser-based Fluorescence Microscopes	Analysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image Quality	Analysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution Microscopy	Discusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)	An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal Microscopy	An overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based Instrumentation	An review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.

LF594/LP-D 594/LP Long-Pass-Filter-Set Laser-Set Di03-R594 Di03-R594-t1 BLP01-594R FF01-591/6 591/6 R594 594R Super-Resolution TIRF PALM STORM SIM Structured-Illumination structured-illumination-microscopy TIRF-illumination-systems P-V Flatness optical-wavefront-distortion reflected-wavefront-distortion Ultra-Flat Filter Filters Set Sets Laser 1WaveRWE RWE LF594/LP-D-000 LF594/LP-D-NTE LF594/LP-D-OMF LF594/LP-D-OFF LF594/LP-D-ZHE

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 594 nm laser sources

Part Number: LF594/LP-D-000

BrightLine® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 594 nm laser sources

BrightLine^® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 594 nm laser sources

BrightLine^® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 594 nm laser sources