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BrightLine^® Pinkel 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 532, & 635 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF405/488/532/635-4X-B-000

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Filters and Pricing
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Fluorophore
Technical Info

Semrock Filter Set: LF405/488/532/635-4X-B

BrightLine^® Pinkel 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 532, & 635 nm laser sources

This "Pinkel" multiband set is optimized for laser excitation over four bands - utilizing a 375 or 405 nm laser source for DAPI, a 473 or 488 nm laser source for GFP or FITC, a 532 nm laser source for TRITC, and a 632.8, 635, or 647.1 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF405/488/532/635-4X-A

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

Part Number

Price¹

Stock Status

Quantity

LF405/488/532/635-4X-B-000 (Standard size set, fits most microscopes²)

$2,325

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-390/40-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF02-482/18-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-532/3-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-640/14-25	$425
Quad Band Emitter	25 mm x 3.5 mm	FF01-446/510/581/703-25	$595
Quad Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R405/488/532/635-t1-25x36	$665

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-390/40-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF02-482/18-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-532/3-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-640/14-25	$425
Quad Band Emitter	25 mm x 3.5 mm	FF01-446/510/581/703-25	$595
Quad Band Dichroic	25.2 mm x 35.6 mm x 3.0 mm	Di03-R405/488/532/635-t3-25x36	$765

Filter Role

Filter Size

Part Number

Price¹

Filter Role

Filter Size

Part Number

Price¹

Filter Role

Filter Size

Part Number

Price¹

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:

These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.

Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LF405/488/532/635-4X-B-NTE (set mounted in cube)

$2,845

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-390/40-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF02-482/18-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-532/3-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-640/14-25	$425
Quad Band Emitter	25 mm x 3.5 mm	FF01-446/510/581/703-25	$595
Quad Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R405/488/532/635-t1-25x36	$665
TE 2000 cube		NTE	$395
Super-resolution flatness certification			$125

Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LF405/488/532/635-4X-B-OMF (set mounted in cube)

$2,885

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-390/40-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF02-482/18-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-532/3-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-640/14-25	$425
Quad Band Emitter	25 mm x 3.5 mm	FF01-446/510/581/703-25	$595
Quad Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R405/488/532/635-t1-25x36	$665
U-MF2 cube		OMF	$435
Super-resolution flatness certification			$125

Olympus U-FF for microscopes: BX53, BX63

LF405/488/532/635-4X-B-OFF (set mounted in cube)

$2,885

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-390/40-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF02-482/18-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-532/3-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-640/14-25	$425
Quad Band Emitter	25 mm x 3.5 mm	FF01-446/510/581/703-25	$595
Quad Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R405/488/532/635-t1-25x36	$665
U-FF cube		OFF	$435
Super-resolution flatness certification			$125

Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LF405/488/532/635-4X-B-ZHE (set mounted in cube)

$2,725

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-390/40-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF02-482/18-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-532/3-25	$375
Single Band Exciter	25 mm x 5.0 mm	FF01-640/14-25	$425
Quad Band Emitter	25 mm x 3.5 mm	FF01-446/510/581/703-25	$595
Quad Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	Di03-R405/488/532/635-t1-25x36	$665
FL Cube EC P+C cube		ZHE	$275
Super-resolution flatness certification			$125

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.
Flatness	Loose and cube mounted filter sets deliver certified dichroic flatness ≤ 1λ P-V RWE @ 632.8 nm

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Blue

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
1,8-ANS	372	477
4-methylumbelliferone	357	441
7-hydroxy-4-methylcoumarin	360	442
Alexa Fluor^® 405	401	422
AMCA (Aminomethylcoumarin)	350	448
ATTO 390	392	477
BD Horizon V450	406	449
BFP (EBFP)	380	440
Calcofluor White	351	444
Cascade Blue™	401	419
CellTrace calcein violet	400	452
DAPI	359	461
DiFMU-pH 9.0	358	450
DY-415	418	470
DyLight 405	398	420
ER-Tracker™ Blue-White DPX	372	557
HiLyte Fluor™ 405	403	428
Hoechst 33258	352	455
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
LysoSensor Blue	374	424
LysoTracker Blue	373	422
Marina Blue	363	461
Pacific Blue™	404	455
sgBFP™	387	451
Sirius	355	424
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Green

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
5-carboxy-2,7-dichlorofluorescein	500	525
5-FAM (5-carboxyfluorescein)	492	518
Acridine Orange (+DNA)	502	526
Alexa Fluor^® 430	431	541
Alexa Fluor^® 488	499	520
Alexa Fluor^® 500	503	525
AmCyan1	458	489
ATTO 488	500	525
ATTO 495	495	527
BCECF (pH 5.5)	481	518
BCECF (pH 9.0)	502	528
BODIPY FL	505	512
BODIPY FL-MeOH	502	511
Calcein	494	514
Calcium Green-1	507	529
CFP2	490	510
Cy2™	492	507
CyQUANT GR-DNA	502	523
DAF-FM-NO	495	519
Dendra2 (Green)	491	507
DiO	475	500
Dronpa	502	516
DY-505-Phalloidin	503	530
ecliptic pHluorin pH5.5	473	507
Emerald	491	511
evoglow-Bs1	449	496
FITC (Fluorescein)	495	519
FlAsH-CCPFCC	511	530
Fluo-3	506	527
Fluo-4	494	516
Fluorescein dextran	501	524
Fluorescein-pH 8.0	489	517
Fluoro-Emerald	494	518
FluoSpheres Yellow-Green fluorescent microspheres	503	514
FM 1-43	473	579
GFP (EGFP)	489	511
Green 496	496	520
Green 500	501	524
HCS LipidTOX Green neutral lipid stain	498	507
HCS LipidTOX Green phospholipidosis	504	536
HiLyte Fluor™ 488	497	526
LIVE-DEAD Fixable Green Dead Cell Stain	498	525
LysoSensor Green	448	502
LysoTracker Green	501	509
LysoTracker Yellow HCK-123	488	565
Magnesium Green	507	531
mHoneyDew	478	562
MitoTracker™ Green	490	512
mWasabi	493	509
NBD-X (MeOH)	467	538
NeuroTrace 500/525 Green Fluorescent Nissl Stain	495	524
Oregon Green™ 488	498	526
Oregon Green™ 514	513	532
PicoGreen^®	502	522
PKH67	488	500
Qdot^® 525 Nanocrystals	300	525
Qdot^® 545 Nanocrystals	300	543
ratiometric pHluorin pH5	500	509
Rhodamine 110	497	519
Rhodamine 123	507	529
Rhodamine Green	497	523
Rhodol Green	497	524
sgGFP™ (super glow GFP)	472	506
Sodium Green	507	532
SpectrumGreen	497	538
SYBR Gold nucleic acid gel stain-DNA	496	539
SYBR Green I nucleic acid gel stain-DNA	498	522
SYBR Safe DNA gel stain-DNA	509	526
SYTO 11	506	525
SYTO 13	448	506
SYTO 16	489	520
SYTO 9	483	500
SYTO RNASelect geen fluorescent cell stain	503	527
SYTOX Green-DNA	504	524
TO-PRO-1	515	531
TOTO-1	514	531
TurboGFP	482	503
Vybrant DyeCycle Green	506	534
wtGFP (wild type GFP, non-UV excitation)	474	509
YO-PRO-1	491	506
YOYO-1	491	508

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Orange

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
2-dodecylresorufin-lipid	582	595
5-ROX (carboxy-X-rhodamine)	578	604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)	542	568
Alexa Fluor^® 546	556	572
Alexa Fluor^® 555	553	565
Alexa Fluor^® 568	579	603
Alexa Fluor^® 594	590	618
Amplex UltraRed peroxidation product (pH 7.5)	568	581
AsRed 2	576	592
ATTO 550	553	576
ATTO 565	563	592
BOBO™-3	570	605
BODIPY TMR-X	544	573
BODIPY TR-X (MeOH)	544	573
BODIPY TR-X phallacidin	590	621
Calcium Crimson	589	609
Calcium Orange	589	609
CellTracker Red CMTPX	586	614
Cy3.5™	578	591
Cy3™	554	566
Dendra2 (Red)	554	569
DiI	551	569
DsRed	559	583
DsRed-Express	556	584
dTomato	556	582
DY-590	582	599
DyLight 594	593	618
Ethidium bromide	518	603
Ethidium homodimer	527	617
FluoSpheres Red fluorescent microspheres	577	607
HCS LipidTOX Red neutral lipid stain	582	616
HCS LipidTOX Red phospholipidosis	584	608
HiLyte Fluor™ 594	592	616
Hypericin	600	603
KFP-Red	575	599
LOLO-1	568	580
LysoTracker Red	573	592
Magnesium Orange	550	574
mApple	566	594
mCherry	587	610
Merocyanine 540	559	579
MitoTracker™ Orange	551	575
MitoTracker™ Red	578	598
mKate2	588	633
mOrange2	551	565
mRFP	555	583
mRFP1	584	700
mRuby	558	605
mStrawberry	574	596
mTangerine	568	585
Nile red-phospolipid	553	637
Orange 552	552	574
pHrodo™, succinimidyl ester	560	587
Propidium Iodide (PI)	305	617
Pro-Q Diamond	556	583
Qdot^® 585 Nanocrystals	300	588
Qdot^® 605 Nanocrystals	300	602
ReAsH-CCPGCC	592	606
Red 580	580	603
Resorufin	571	584
Rhod-2	553	577
Rhodamine phalloidin	558	575
Rhodamine Red-X	573	591
R-phycoerythrin (PE)	565	576
SNARF (carboxy) 514 Excitation pH9	576	638
SNARF-1 488nm (ph 6.0)	548	586
SNARF-1 488nm (ph 9.0)	576	638
SNARF-1 514nm (ph 6.0)	549	587
SNARF-1 514nm (ph 9.0)	576	638
SpectrumOrange	554	587
Sulforhodamine 101-EtOH	578	593
SYTOX Orange	547	570
Tetramethylrhodamine dextran	554	582
Texas Red dextran	592	613
Texas Red DHPE	584	608
TRITC (Tetramethylrhodamine)	552	578
TurboRFP	553	573
X-Rhod-1 Indicator	580	601

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Red

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
5-carboxynapthofluorescein (pH 10)	555	615
Alexa Fluor^® 633	631	647
Alexa Fluor^® 635	633	648
Alexa Fluor^® 647	653	668
Alexa Fluor^® 660	663	691
APC	651	660
APC (Allophycocyanin)	651	660
ATTO 633	629	658
ATTO 635	635	658
ATTO 647	644	670
ATTO 647N	645	668
ATTO 655	663	683
BODIPY 650/665-X	646	664
Carboxynaphthofluorescein	599	674
Cy5.5™	672	690
Cy5™	649	666
DDAO	648	657
DiD (DiIC18(5)) - Lipophilic Tracer	648	670
DY-630	634	656
DyLight 633	620	649
DyLight 649	651	673
FluoSpheres Crimson fluorescent microspheres	620	646
FluoSpheres Dark Red fluorescent microspheres	656	683
HCS CellMask Red cytoplasmic	622	644
HCS LipidTOX Deep Red neutral lipid stain	635	652
HiLyte Fluor™ 647	649	673
LIVE-DEAD Fixable Far Red Dead Cell Stain	653	668
MitoTracker™ Deep Red	641	662
Nile blue-EtOH	631	660
Qdot^® 705 Nanocrystals	300	702
Red 650	649	665
SpectrumFRed (Far Red)	650	673
SYTO 59	622	644
SYTO 60	650	681
SYTO 61	618	650
SYTOX Red-DNA	640	658
TO-PRO-3	642	657
TOTO-3	642	661

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic Beamsplitters	Describes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set Terminology	Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Optical Filters for Laser-based Fluorescence Microscopes	Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection

Journal Article

Topic	Description
Bringing Super-resolution to Fluorescence Microscopy	Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010 Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systems	Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
New Optical Filters Improve High-Speed Multicolor Fluorescence Imaging	Turan Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006 Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
Perfecting TIRF Optics	Prashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009 Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection

White Paper

Topic	Description
Optical Filters for Laser-based Fluorescence Microscopes	Analysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Super-resolution Microscopy	Discusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)	An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal Microscopy	An overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based Instrumentation	An review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.

LF405/488/532/635-4X-B 405/488/532/635 Pinkel Laser-Set Quad-Band FF01-446/510/581/703 FF01-390/40 Di03-R405/488/532/635 Di03-R405/488/532/635-t1 FF01-532/3 FF01-640/14 FF02-482/18 446/510/581/703 390/40 R405/488/532/635 532/3 640/14 482/18 Super-Resolution TIRF PALM STORM SIM Structured-Illumination structured-illumination-microscopy TIRF-illumination-systems P-V Flatness optical-wavefront-distortion reflected-wavefront-distortion Ultra-Flat Filter Filters Set Sets Laser Quad 1WaveRWE RWE 4X LF405/488/532/635-4X-B-000 LF405/488/532/635-4X-B-NTE LF405/488/532/635-4X-B-OMF LF405/488/532/635-4X-B-OFF LF405/488/532/635-4X-B-ZHE

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® Pinkel 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 532, & 635 nm laser sources

Part Number: LF405/488/532/635-4X-B-000

BrightLine® Pinkel 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 532, & 635 nm laser sources

BrightLine^® Pinkel 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 532, & 635 nm laser sources

BrightLine^® Pinkel 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 532, & 635 nm laser sources