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BrightLine® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 405 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF405/LP-C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: LF405/LP-C

BrightLine® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 405 nm laser sources

This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from DAPI and other similar fluorophores while offering superb blocking of popular 405 nm or 375 nm solid-state laser diodes. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination.

Loose and cube mounted filter sets are certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm.

Looking for this filter set in one of the following cubes: NQF, LDMK or LDMP?
Our previous version is still available for those cube configurations LF405/LP-B


Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LF405/LP-C-000 (Standard size set, fits most microscopes2)$965In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-390/40-25$305
Long Pass Emitter25 mm x 3.5 mmBLP01-405R-25$355
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmDi03-R405-t1-25x36$445

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LF405/LP-C-NTE (set mounted in cube)$1,485Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LF405/LP-C-OMF (set mounted in cube)$1,525In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LF405/LP-C-OFF (set mounted in cube)$1,525In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LF405/LP-C-ZHE (set mounted in cube)$1,365In StockAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724773-Star
4-methylumbelliferone3574411-Star
7-hydroxy-4-methylcoumarin3604423-Star
Alexa Fluor® 3503434413-Star
Alexa Fluor® 4054014225-Star
Alexa Fluor® 4304315413-Star
AMCA (Aminomethylcoumarin)3504483-Star
Aqua 4314304781-Star
ATTO 3903924775-Star
ATTO 4254364841-Star
BD Horizon V4504064495-Star
BD Horizon V5004105003-Star
BFP (EBFP)3804405-Star
BV421 - BD Horizon Brilliant™ Violet 4214074215-Star
Calcofluor White3514441-Star
Cascade Blue™4014195-Star
Cascade Yellow™3995495-Star
CellTrace calcein violet4004525-Star
CellTracker Violet BMQC+GSH4065265-Star
CFP (ECFP)4334751-Star
ChlorophyllA4806503-Star
DAPI3594615-Star
Dapoxyl3735795-Star
DEAC4324723-Star
DiFMU-pH 9.03584501-Star
DY-4154184705-Star
DyLight 3503514343-Star
DyLight 4053984205-Star
ER-Tracker™ Blue-White DPX3725575-Star
Fura Red™ (high pH)5726573-Star
Fura-2 (calcium free)3665153-Star
GFPuv3955093-Star
HiLyte Fluor™ 4054034285-Star
Hoechst 332583524551-Star
Hoechst 333423524553-Star
Hoechst 345803924403-Star
LIVE-DEAD Fixable Aqua Dead Cell Stain3675265-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034555-Star
Lucifer yellow4285443-Star
LysoSensor Blue3744245-Star
LysoTracker Blue3734223-Star
Marina Blue3634615-Star
mBBr+GSH3944905-Star
Pacific Blue™4044555-Star
Pacific Orange4005515-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005433-Star
Qdot® 565 Nanocrystals3005643-Star
Qdot® 585 Nanocrystals3005883-Star
Qdot® 605 Nanocrystals3006023-Star
Qdot® 625 Nanocrystals3006213-Star
Qdot® 655 Nanocrystals3006543-Star
Qdot® 705 Nanocrystals3007023-Star
Qdot® 800 Nanocrystals3007923-Star
sgBFP™3874515-Star
Sirius3554243-Star
SpectrumAqua4344811-Star
SpectrumBlue4054495-Star
SYPRO Ruby protein gel stain4696313-Star
TagBFP4024575-Star
ThiolTracker Violet+GSH4045265-Star
Vybrant DyeCycle Violet3694373-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.



LF405/LP-C 405/LP Long-Pass-Filter-Set Laser-Set FF01-390/40 BLP01-405R Di03-R405 Di03-R405-t1 390/40 405R R405 Super-Resolution TIRF PALM STORM SIM Structured-Illumination structured-illumination-microscopy TIRF-illumination-systems P-V Flatness optical-wavefront-distortion reflected-wavefront-distortion Ultra-Flat Filter Filters Set Sets Laser 1WaveRWE RWE LF405/LP-C-000 LF405/LP-C-NTE LF405/LP-C-OMF LF405/LP-C-OFF LF405/LP-C-ZHE

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