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SetDetails

BrightLine® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

Semrock Set / Fluorophore Graphing

Part Number: LED-CFP/YFP/mCherry-3X3M-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: LED-CFP/YFP/mCherry-3X3M-A

BrightLine® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 7-filter "Sedat" triple-band set is designed for high-speed, sequential imaging of CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-000 (Standard size set, fits most microscopes2)$2,195Confirmed AROAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-438/24-25$325
Single Band Exciter25 mm x 5.0 mmFF01-509/22-25$355
Single Band Exciter25 mm x 5.0 mmFF01-578/21-25$355
Single Band Emitter25 mm x 3.5 mmFF01-482/25-25$325
Single Band Emitter25 mm x 3.5 mmFF01-544/24-25$355
Single Band Emitter25 mm x 3.5 mmFF02-641/75-25$325
Triple Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF459/526/596-Di01-25x36$465
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-L01 (Leica 'Large' set)$2,195Confirmed AROAdd To Cart
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-S01 (Sutter threaded set)$2,315Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-NQF (set mounted in cube)$2,590Confirmed AROAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-NTE (set mounted in cube)$2,590Confirmed AROAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-OMF (set mounted in cube)$2,630Confirmed AROAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-OFF (set mounted in cube)$2,630Confirmed AROAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-ZHE (set mounted in cube)$2,470Confirmed AROAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-LDMK (set mounted in cube)$2,545Confirmed AROAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LED-CFP/YFP/mCherry-3X3M-A-LDMP (set mounted in cube)$2,630Confirmed AROAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, and Zeiss microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Cyan
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Acridine Yellow4705501-Star
Aqua 4314304785-Star
ATTO 4254364845-Star
ATTO 4654535071-Star
BD Horizon V5004105001-Star
BOBO™-14614843-Star
BV480 - BD Horizon Brilliant™ Violet 4804404795-Star
Cerulean4344735-Star
CFP (ECFP)4334755-Star
DY-4154184703-Star
ECFP4354755-Star
mBBr+GSH3944901-Star
mTFP14624921-Star
POPO-14334575-Star
PO-PRO-14334561-Star
SpectrumAqua4344815-Star
SYTO 454514853-Star
SYTOX Blue4444705-Star
TagCFP4584805-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Yellow
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925181-Star
6-carboxyrhodamine 6G5255555-Star
6-JOE5205485-Star
Acridine Orange (+DNA)5025265-Star
Alexa Fluor® 4884995201-Star
Alexa Fluor® 5005035251-Star
Alexa Fluor® 5145185435-Star
Alexa Fluor® 5325345531-Star
ATTO 4885005253-Star
ATTO 4954955275-Star
ATTO 5205165385-Star
ATTO 5325015451-Star
BODIPY R6G5285473-Star
Calcein4945141-Star
Calcium Green-15075295-Star
CyQUANT GR-DNA5025233-Star
DAF-FM-NO4955191-Star
Dronpa5025161-Star
DY-505-Phalloidin5035305-Star
DyLight 4884935181-Star
Eosin5255465-Star
FITC (Fluorescein)4955191-Star
FlAsH-CCPFCC5115305-Star
Fluo-35065275-Star
Fluo-44945161-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895171-Star
Fluoro-Emerald4945183-Star
FluoSpheres Orange fluorescent microspheres5405591-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
FM 1-434735791-Star
Gold 5255215515-Star
Green 4964965201-Star
Green 5005015243-Star
HCS LipidTOX Green phospholipidosis5045365-Star
HiLyte Fluor™ 4884975263-Star
JO-JO-1, JO-PRO-15305443-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075315-Star
mHoneyDew4785625-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135325-Star
PicoGreen®5025221-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
Rhodamine 1104975191-Star
Rhodamine 1235075293-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
Sodium Green5075325-Star
SpectrumGreen4975381-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYBR Safe DNA gel stain-DNA5095263-Star
SYTO 115065253-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TO-PRO-15155315-Star
TOTO-15145315-Star
TurboYFP5255383-Star
Venus5165285-Star
VIC5305501-Star
Vybrant DyeCycle Green5065345-Star
Vybrant DyeCycle Orange5195633-Star
YFP (yellow GFP)5135305-Star
ZsYellow15295393-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825951-Star
5-carboxynapthofluorescein (pH 10)5556155-Star
5-ROX (carboxy-X-rhodamine)5786045-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5685796035-Star
Alexa Fluor® 5945906185-Star
Alexa Fluor® 6606636911-Star
APC (Allophycocyanin)6516601-Star
AsRed 25765925-Star
ATTO 5655635921-Star
ATTO 5905946245-Star
ATTO 5946016243-Star
ATTO 6106146341-Star
BOBO™-35706055-Star
BODIPY TR-X phallacidin5906215-Star
Calcium Crimson5896095-Star
Carboxynaphthofluorescein5996745-Star
CellTrace BODIPY TR methyl ester5986253-Star
CellTracker Red CMTPX5866145-Star
Cy3.5™5785911-Star
DDAO6486571-Star
DY-5905825993-Star
DY-6306346561-Star
DyLight 5945936185-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Red fluorescent microspheres5776075-Star
FM 4-645057251-Star
HcRed15886183-Star
HCS LipidTOX Deep Red neutral lipid stain6356521-Star
HCS LipidTOX Red neutral lipid stain5826165-Star
HCS LipidTOX Red phospholipidosis5846085-Star
HiLyte Fluor™ 5945926165-Star
KFP-Red5755995-Star
LDS 7515607115-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956135-Star
LysoTracker Red5735923-Star
mApple5665943-Star
mCherry5876105-Star
MitoTracker™ Red5785985-Star
mPlum5896495-Star
mRFP15847005-Star
mRuby5586051-Star
mStrawberry5745963-Star
Nile blue-EtOH6316601-Star
Nile red-phospolipid5536373-Star
PerCP4906771-Star
Propidium Iodide (PI)3056173-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Qdot® 655 Nanocrystals3006541-Star
Qdot® 705 Nanocrystals3007021-Star
Qdot® 800 Nanocrystals3007921-Star
ReAsH-CCPGCC5926065-Star
Red 5805806035-Star
Rhodamine Red-X5735911-Star
SNARF (carboxy) 514 Excitation pH95766385-Star
SNARF-1 488nm (ph 9.0)5766385-Star
SNARF-1 514nm (ph 9.0)5766385-Star
SpectrumRed5876153-Star
Sulforhodamine 101-EtOH5785931-Star
SYTO 176196381-Star
SYTO 616186501-Star
Texas Red dextran5926135-Star
Texas Red DHPE5846085-Star
Texas Red-X®5956135-Star
TurboFP635(Katushka)5916385-Star
X-Rhod-1 Indicator5806015-Star
YO-PRO-36136291-Star
YOYO-36116311-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



LED-CFP/YFP/mCherry-3X3M-A LED 3X3M CFP YFP mCherry CFP/YFP/mCherry LED Sedat Triple-Band 438/24 FF02-438/24 509/22 FF01-509/22 578/21 FF01-578/21 482/25 FF01-482/25 544/24 FF01-544/24 641/75 FF02-641/75 459/526/596 FF459/526/596-Di01 FF459/526/596 Lumencor Sola Spectra-X CoolLED pE-4000 X-Cite Turbo XLED1 120LED-Broadband Filter Filters Set Sets Triple 3X3M LED-CFP/YFP/mCherry-3X3M-A-000 LED-CFP/YFP/mCherry-3X3M-A-L01 LED-CFP/YFP/mCherry-3X3M-A-S01 LED-CFP/YFP/mCherry-3X3M-A-NQF LED-CFP/YFP/mCherry-3X3M-A-NTE LED-CFP/YFP/mCherry-3X3M-A-OMF LED-CFP/YFP/mCherry-3X3M-A-OFF LED-CFP/YFP/mCherry-3X3M-A-ZHE LED-CFP/YFP/mCherry-3X3M-A-LDMP LED-CFP/YFP/mCherry-3X3M-A-LDMK

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