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BrightLine^® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

Semrock Set / Fluorophore Graphing

Part Number: LED-CFP/YFP/mCherry-3X3M-A-000

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Filters and Pricing
Specifications
Fluorophore
Technical Info

Semrock Filter Set: LED-CFP/YFP/mCherry-3X3M-A

BrightLine^® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This 7-filter "Sedat" triple-band set is designed for high-speed, sequential imaging of CFP, YFP, and mCherry (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter with three single-band emitters intended to be mounted in filter wheels and three single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

Part Number

Price¹

Stock Status

Quantity

LED-CFP/YFP/mCherry-3X3M-A-000 (Standard size set, fits most microscopes²)

$2,195

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-438/24-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-578/21-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-482/25-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-641/75-25	$345
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF459/526/596-Di01-25x36	$495

LED-CFP/YFP/mCherry-3X3M-A-L01 (Leica 'Large' set)

$2,195

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-438/24-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-578/21-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-482/25-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-641/75-25	$345
Triple Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF459/526/596-Di01-22x29	$495

LED-CFP/YFP/mCherry-3X3M-A-S01 (Sutter threaded set)

$2,315

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF02-438/24-25-STR	$365
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-509/22-25-STR	$365
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-578/21-25-STR	$365
Single Band Emitter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-482/25-25-STR	$365
Single Band Emitter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-544/24-25-STR	$365
Single Band Emitter	25 mm threaded ring for Sutter Lambda filter wheel	FF02-641/75-25-STR	$365
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF459/526/596-Di01-25x36	$495

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:

These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.

Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LED-CFP/YFP/mCherry-3X3M-A-NQF (set mounted in cube)

$2,590

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-438/24-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-578/21-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-482/25-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-641/75-25	$345
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF459/526/596-Di01-25x36	$495
Quadfluor cube		NQF	$395

Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LED-CFP/YFP/mCherry-3X3M-A-NTE (set mounted in cube)

$2,590

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-438/24-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-578/21-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-482/25-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-641/75-25	$345
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF459/526/596-Di01-25x36	$495
TE 2000 cube		NTE	$395

Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LED-CFP/YFP/mCherry-3X3M-A-OMF (set mounted in cube)

$2,630

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-438/24-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-578/21-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-482/25-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-641/75-25	$345
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF459/526/596-Di01-25x36	$495
U-MF2 cube		OMF	$435

Olympus U-FF for microscopes: BX53, BX63

LED-CFP/YFP/mCherry-3X3M-A-OFF (set mounted in cube)

$2,630

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-438/24-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-578/21-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-482/25-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-641/75-25	$345
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF459/526/596-Di01-25x36	$495
U-FF cube		OFF	$435

Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LED-CFP/YFP/mCherry-3X3M-A-ZHE (set mounted in cube)

$2,470

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-438/24-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-578/21-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-482/25-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-641/75-25	$345
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF459/526/596-Di01-25x36	$495
FL Cube EC P+C cube		ZHE	$275

Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B

LED-CFP/YFP/mCherry-3X3M-A-LDMK (set mounted in cube)

$2,545

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-438/24-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-578/21-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-482/25-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-641/75-25	$345
Triple Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF459/526/596-Di01-22x29	$495
DM-K cube		LDMK	$350

Leica DMi8 (P-Cube) for microscopes: DMi8

LED-CFP/YFP/mCherry-3X3M-A-LDMP (set mounted in cube)

$2,630

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-438/24-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Exciter	25 mm x 5.0 mm	FF01-578/21-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-482/25-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF02-641/75-25	$345
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF459/526/596-Di01-25x36	$495
DMi8 (P-Cube) cube		LDMP	$435

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, and Zeiss microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Cyan

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
Acridine Yellow	470	550
Aqua 431	430	478
ATTO 425	436	484
ATTO 465	453	507
BD Horizon V500	410	500
BOBO™-1	461	484
BV480 - BD Horizon Brilliant™ Violet 480	440	479
Cerulean	434	473
CFP (ECFP)	433	475
DY-415	418	470
ECFP	435	475
mBBr+GSH	394	490
mTFP1	462	492
POPO-1	433	457
PO-PRO-1	433	456
SpectrumAqua	434	481
SYTO 45	451	485
SYTOX Blue	444	470
TagCFP	458	480

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Yellow

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
5-carboxy-2,7-dichlorofluorescein	500	525
5-FAM (5-carboxyfluorescein)	492	518
6-carboxyrhodamine 6G	525	555
6-JOE	520	548
Acridine Orange (+DNA)	502	526
Alexa Fluor^® 488	499	520
Alexa Fluor^® 500	503	525
Alexa Fluor^® 514	518	543
Alexa Fluor^® 532	534	553
ATTO 488	500	525
ATTO 495	495	527
ATTO 520	516	538
ATTO 532	501	545
BODIPY R6G	528	547
Calcein	494	514
Calcium Green-1	507	529
CyQUANT GR-DNA	502	523
DAF-FM-NO	495	519
Dronpa	502	516
DY-505-Phalloidin	503	530
DyLight 488	493	518
Eosin	525	546
FITC (Fluorescein)	495	519
FlAsH-CCPFCC	511	530
Fluo-3	506	527
Fluo-4	494	516
Fluorescein dextran	501	524
Fluorescein-pH 8.0	489	517
Fluoro-Emerald	494	518
FluoSpheres Orange fluorescent microspheres	540	559
FluoSpheres Yellow-Green fluorescent microspheres	503	514
FM 1-43	473	579
Gold 525	521	551
Green 496	496	520
Green 500	501	524
HCS LipidTOX Green phospholipidosis	504	536
HiLyte Fluor™ 488	497	526
JO-JO-1, JO-PRO-1	530	544
LIVE-DEAD Fixable Green Dead Cell Stain	498	525
LysoTracker Yellow HCK-123	488	565
Magnesium Green	507	531
mHoneyDew	478	562
NeuroTrace 500/525 Green Fluorescent Nissl Stain	495	524
Oregon Green™ 488	498	526
Oregon Green™ 514	513	532
PicoGreen^®	502	522
Qdot^® 525 Nanocrystals	300	525
Qdot^® 545 Nanocrystals	300	543
Rhodamine 110	497	519
Rhodamine 123	507	529
Rhodamine Green	497	523
Rhodol Green	497	524
Sodium Green	507	532
SpectrumGreen	497	538
SYBR Gold nucleic acid gel stain-DNA	496	539
SYBR Green I nucleic acid gel stain-DNA	498	522
SYBR Safe DNA gel stain-DNA	509	526
SYTO 11	506	525
SYTO RNASelect geen fluorescent cell stain	503	527
SYTOX Green-DNA	504	524
TO-PRO-1	515	531
TOTO-1	514	531
TurboYFP	525	538
Venus	516	528
VIC	530	550
Vybrant DyeCycle Green	506	534
Vybrant DyeCycle Orange	519	563
YFP (yellow GFP)	513	530
ZsYellow1	529	539

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Red

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
2-dodecylresorufin-lipid	582	595
5-carboxynapthofluorescein (pH 10)	555	615
5-ROX (carboxy-X-rhodamine)	578	604
7-AAD (7-aminoactinomycin D)	548	648
Alexa Fluor^® 568	579	603
Alexa Fluor^® 594	590	618
Alexa Fluor^® 660	663	691
APC (Allophycocyanin)	651	660
AsRed 2	576	592
ATTO 565	563	592
ATTO 590	594	624
ATTO 594	601	624
ATTO 610	614	634
BOBO™-3	570	605
BODIPY TR-X phallacidin	590	621
Calcium Crimson	589	609
Carboxynaphthofluorescein	599	674
CellTrace BODIPY TR methyl ester	598	625
CellTracker Red CMTPX	586	614
Cy3.5™	578	591
DDAO	648	657
DY-590	582	599
DY-630	634	656
DyLight 594	593	618
FluoSpheres Crimson fluorescent microspheres	620	646
FluoSpheres Red fluorescent microspheres	577	607
FM 4-64	505	725
HcRed1	588	618
HCS LipidTOX Deep Red neutral lipid stain	635	652
HCS LipidTOX Red neutral lipid stain	582	616
HCS LipidTOX Red phospholipidosis	584	608
HiLyte Fluor™ 594	592	616
KFP-Red	575	599
LDS 751	560	711
LIVE-DEAD Fixable Red Dead Cell Stain	595	613
LysoTracker Red	573	592
mApple	566	594
mCherry	587	610
MitoTracker™ Red	578	598
mPlum	589	649
mRFP1	584	700
mRuby	558	605
mStrawberry	574	596
Nile blue-EtOH	631	660
Nile red-phospolipid	553	637
PerCP	490	677
Propidium Iodide (PI)	305	617
Qdot^® 605 Nanocrystals	300	602
Qdot^® 625 Nanocrystals	300	621
Qdot^® 655 Nanocrystals	300	654
Qdot^® 705 Nanocrystals	300	702
Qdot^® 800 Nanocrystals	300	792
ReAsH-CCPGCC	592	606
Red 580	580	603
Rhodamine Red-X	573	591
SNARF (carboxy) 514 Excitation pH9	576	638
SNARF-1 488nm (ph 9.0)	576	638
SNARF-1 514nm (ph 9.0)	576	638
SpectrumRed	587	615
Sulforhodamine 101-EtOH	578	593
SYTO 17	619	638
SYTO 61	618	650
Texas Red dextran	592	613
Texas Red DHPE	584	608
Texas Red-X^®	595	613
TurboFP635(Katushka)	591	638
X-Rhod-1 Indicator	580	601
YO-PRO-3	613	629
YOYO-3	611	631

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set Terminology	Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets

Journal Article

Topic	Description
New Optical Filters Improve High-Speed Multicolor Fluorescence Imaging	Turan Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006 Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging

White Paper

Topic	Description
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.

LED-CFP/YFP/mCherry-3X3M-A LED 3X3M CFP YFP mCherry CFP/YFP/mCherry LED Sedat Triple-Band 438/24 FF02-438/24 509/22 FF01-509/22 578/21 FF01-578/21 482/25 FF01-482/25 544/24 FF01-544/24 641/75 FF02-641/75 459/526/596 FF459/526/596-Di01 FF459/526/596 Lumencor Sola Spectra-X CoolLED pE-4000 X-Cite Turbo XLED1 120LED-Broadband Filter Filters Set Sets Triple 3X3M LED-CFP/YFP/mCherry-3X3M-A-000 LED-CFP/YFP/mCherry-3X3M-A-L01 LED-CFP/YFP/mCherry-3X3M-A-S01 LED-CFP/YFP/mCherry-3X3M-A-NQF LED-CFP/YFP/mCherry-3X3M-A-NTE LED-CFP/YFP/mCherry-3X3M-A-OMF LED-CFP/YFP/mCherry-3X3M-A-OFF LED-CFP/YFP/mCherry-3X3M-A-ZHE LED-CFP/YFP/mCherry-3X3M-A-LDMP LED-CFP/YFP/mCherry-3X3M-A-LDMK

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

Part Number: LED-CFP/YFP/mCherry-3X3M-A-000

BrightLine® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

BrightLine^® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines

BrightLine^® Sedat filter set, optimized for CFP, YFP, & mCherry and other like fluorophores, illuminated with LED-based light engines