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SetDetails

BrightLine® single-band filter set, optimized for Brilliant™ Violet 480 and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: BV480-2432A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: BV480-2432A

BrightLine® single-band filter set, optimized for Brilliant™ Violet 480 and other like fluorophores

This filter set is ideal for use with Brilliant™ Violet 480 and a variety of other blue fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio. Are you co-labeling with BUV395, BV421? This filter set is optimized to minimize crosstalk with BUV395 and BV421.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details BV480-2432A-000 (Standard size set, fits most microscopes2)$825Confirmed AROAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-438/24-25$325
Single Band Emitter25 mm x 3.5 mmFF01-483/32-25$325
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF458-Di02-25x36$275
Click to expand/collapse details BV480-2432A-000-ZERO (Standard size -ZERO pixel set)$924Confirmed AROAdd To Cart
Click to expand/collapse details BV480-2432A-L01-ZERO (Leica 'Large' -ZERO pixel set)$924Confirmed AROAdd To Cart
Click to expand/collapse details BV480-2432A-U02 (Olympus large-size (FX) set for U-M710I)$1,370Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details BV480-2432A-NQF (set mounted in cube)$1,220Confirmed AROAdd To Cart
Click to expand/collapse details BV480-2432A-NQF-ZERO (-ZERO pixel set mounted in cube)$1,319Confirmed AROAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details BV480-2432A-NTE (set mounted in cube)$1,220Confirmed AROAdd To Cart
Click to expand/collapse details BV480-2432A-NTE-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details BV480-2432A-OMF (set mounted in cube)$1,260Confirmed AROAdd To Cart
Click to expand/collapse details BV480-2432A-OMF-ZERO (-ZERO pixel set mounted in cube)$1,359Confirmed AROAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details BV480-2432A-OFF (set mounted in cube)$1,260Confirmed AROAdd To Cart
Click to expand/collapse details BV480-2432A-OFF-ZERO (-ZERO pixel set mounted in cube)$1,359Confirmed AROAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details BV480-2432A-OFX (set mounted in cube)$2,065Confirmed AROAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details BV480-2432A-ZHE (set mounted in cube)$1,100Confirmed AROAdd To Cart
Click to expand/collapse details BV480-2432A-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,199Confirmed AROAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details BV480-2432A-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,274Confirmed AROAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details BV480-2432A-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,359Confirmed AROAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details BV480-2432A-AMF-ZERO (-ZERO pixel set mounted in cube)$1,389Confirmed AROAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Acridine Yellow4705501-Star
AmCyan14584891-Star
Aqua 4314304785-Star
ATTO 4254364845-Star
ATTO 4654535071-Star
BD Horizon V5004105001-Star
BOBO™-14614843-Star
BV480 - BD Horizon Brilliant™ Violet 4804404795-Star
Cerulean4344735-Star
CFP (ECFP)4334755-Star
DY-4154184701-Star
ECFP4354755-Star
evoglow-Bs14494963-Star
LysoSensor Green4485021-Star
mBBr+GSH3944901-Star
mTFP14624923-Star
POPO-14334571-Star
PO-PRO-14334561-Star
SpectrumAqua4344815-Star
SYTO 454514855-Star
SYTOX Blue4444705-Star
TagCFP4584805-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



BV480-2432A BV480 Brilliant Violet BrilliantViolet 480 438/24 483/32 458 Single-Band FF458 FF458-Di02 FF02-438/24 FF01-483/32 Filter Filters Set Sets BV480-2432A-000 BV480-2432A-000-ZERO BV480-2432A-L01-ZERO BV480-2432A-U02 BV480-2432A-NQF BV480-2432A-NQF-ZERO BV480-2432A-NTE BV480-2432A-NTE-ZERO BV480-2432A-OMF BV480-2432A-OMF-ZERO BV480-2432A-OFF BV480-2432A-OFF-ZERO BV480-2432A-OFX BV480-2432A-ZHE BV480-2432A-ZHE-ZERO BV480-2432A-LDMP-ZERO BV480-2432A-LDMK-ZERO BV480-2432A-AMF-ZERO

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