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BrightLine® single-band filter set, optimized for Brilliant™ Ultraviolet 395 and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: BUV395-3018A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: BUV395-3018A

BrightLine® single-band filter set, optimized for Brilliant™ Ultraviolet 395 and other like fluorophores

Unique UV fluorescence filter set! Only Semrock offers true UV fluorescence filters with high transmission, edge steepness, and blocking that rival high-performance visible filters. This filter set is optimized to be simultaneously bright and high signal-to-noise ratio (contrast) for measuring Brilliant™ Ultraviolet 395 and other UV-excited fluorescent species. This set is optimized for systems using a light source such as a UV LED or Xe arc lamp. Are you co-labeling with BV421? This filter set is optimized to minimize crosstalk with BV421.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details BUV395-3018A-000 (Standard size set, fits most microscopes2)$785In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-356/30-25$355
Single Band Emitter25 mm x 3.5 mmFF01-392/18-25$405
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF376-Di01-25x36$255
Click to expand/collapse details BUV395-3018A-000-ZERO (Standard size -ZERO pixel set)$884In StockAdd To Cart
Click to expand/collapse details BUV395-3018A-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details BUV395-3018A-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details BUV395-3018A-NQF (set mounted in cube)$1,180Ships in 2 daysAdd To Cart
Click to expand/collapse details BUV395-3018A-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details BUV395-3018A-NTE (set mounted in cube)$1,180Ships in 2 daysAdd To Cart
Click to expand/collapse details BUV395-3018A-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details BUV395-3018A-OMF (set mounted in cube)$1,220Ships in 2 daysAdd To Cart
Click to expand/collapse details BUV395-3018A-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details BUV395-3018A-OFF (set mounted in cube)$1,220Ships in 2 daysAdd To Cart
Click to expand/collapse details BUV395-3018A-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details BUV395-3018A-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details BUV395-3018A-ZHE (set mounted in cube)$1,060Ships in 2 daysAdd To Cart
Click to expand/collapse details BUV395-3018A-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details BUV395-3018A-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details BUV395-3018A-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details BUV395-3018A-AMF-ZERO (-ZERO pixel set mounted in cube)$1,349Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
BUV395 - BD Horizon Brilliant™ Ultraviolet 3953483955-Star
FluoSpheres Blue fluorescent microspheres3564143-Star
LysoSensor Blue3744241-Star
LysoTracker Blue3734221-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



BUV395-3018A BUV395 Brilliant Ultraviolet BrilliantUltraViolet 395 356/30 392/18 376 Single-Band FF01-356/30 FF01-392/18 FF376 FF376-Di01 Filter Filters Set Sets BUV395-3018A-000 BUV395-3018A-000-ZERO BUV395-3018A-L01-ZERO BUV395-3018A-U02 BUV395-3018A-NQF BUV395-3018A-NQF-ZERO BUV395-3018A-NTE BUV395-3018A-NTE-ZERO BUV395-3018A-OMF BUV395-3018A-OMF-ZERO BUV395-3018A-OFF BUV395-3018A-OFF-ZERO BUV395-3018A-OFX BUV395-3018A-ZHE BUV395-3018A-ZHE-ZERO BUV395-3018A-LDMP-ZERO BUV395-3018A-LDMK-ZERO BUV395-3018A-AMF-ZERO

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