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BrightLine^® single-band filter set, optimized for YFP and other like fluorophores, illuminated with LED based light sources

Semrock Set / Fluorophore Graphing

Part Number: LED-YFP-A-000

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Filters and Pricing
Specifications
Fluorophore
Technical Info

BrightLine^® single-band filter set, optimized for YFP and other like fluorophores, illuminated with LED based light sources

Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings.

This filter set is optimized to simultaneously deliver bright signal and high signal-to-noise ratio (contrast) for measuring YFP (or other key fluorophores – see complete list above). This set is spectrally aligned for excitation with many of the broadband or discrete LED-based light engines available in the market.

Interested in learning how this filter set will perform with your light source? Try Semrock's SearchLight plotting & calculation tool.

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

Part Number

Price¹

Stock Status

Quantity

LED-YFP-A-000 (Standard size set, fits most microscopes²)

$825

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355

LED-YFP-A-000-ZERO (Standard size -ZERO pixel set)

$924

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
-ZERO set certification		-ZERO	$99

LED-YFP-A-L01-ZERO (Leica 'Large' -ZERO pixel set)

$924

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	21.8 mm x 2.0 mm	FF01-509/22-21.8-D	$345
Single Band Emitter	23.3 mm x 2.0 mm	FF01-544/24-23.3-D	$345
Single Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF526-Di01-22x29	$355
-ZERO set certification		-ZERO	$99

Filter Role	Filter Size	Part Number	Price¹
-ZERO set certification		-ZERO	$99

LED-YFP-A-U02 (Olympus large-size (FX) set for U-M710I)

$1,370

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	32 mm x 5.0 mm	FF01-509/22-32	$565
Single Band Emitter	32 mm x 3.5 mm	FF01-544/24-32	$565
Single Band Dichroic	32 x 44 x 1.1 mm (corners cut for OFX cube)	FF526-Di01-32x44-FX	$557

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:

These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.

Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

LED-YFP-A-NQF (set mounted in cube)

$1,220

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
Quadfluor cube		NQF	$395

LED-YFP-A-NQF-ZERO (-ZERO pixel set mounted in cube)

$1,319

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
Quadfluor cube		NQF	$395
-ZERO set certification		-ZERO	$99

Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

LED-YFP-A-NTE (set mounted in cube)

$1,220

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
TE 2000 cube		NTE	$395

LED-YFP-A-NTE-ZERO (-ZERO pixel set mounted in cube)

$1,319

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
TE 2000 cube		NTE	$395
-ZERO set certification		-ZERO	$99

Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

LED-YFP-A-OMF (set mounted in cube)

$1,260

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
U-MF2 cube		OMF	$435

LED-YFP-A-OMF-ZERO (-ZERO pixel set mounted in cube)

$1,359

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
U-MF2 cube		OMF	$435
-ZERO set certification		-ZERO	$99

Olympus U-FF for microscopes: BX53, BX63

LED-YFP-A-OFF (set mounted in cube)

$1,260

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
U-FF cube		OFF	$435

LED-YFP-A-OFF-ZERO (-ZERO pixel set mounted in cube)

$1,359

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
U-FF cube		OFF	$435
-ZERO set certification		-ZERO	$99

Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes

LED-YFP-A-OFX (set mounted in cube)

$2,065

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	32 mm x 5.0 mm	FF01-509/22-32	$565
Single Band Emitter	32 mm x 3.5 mm	FF01-544/24-32	$565
Single Band Dichroic	32 x 44 x 1.1 mm (corners cut for OFX cube)	FF526-Di01-32x44-FX	$557
U-M710I cube		OFX	$695

Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

LED-YFP-A-ZHE (set mounted in cube)

$1,100

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
FL Cube EC P+C cube		ZHE	$275

LED-YFP-A-ZHE-ZERO (-ZERO pixel set mounted in cube)

$1,199

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
FL Cube EC P+C cube		ZHE	$275
-ZERO set certification		-ZERO	$99

Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B

LED-YFP-A-LDMK-ZERO (-ZERO pixel set mounted in cube)

$1,274

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	21.8 mm x 2.0 mm	FF01-509/22-21.8-D	$345
Single Band Emitter	23.3 mm x 2.0 mm	FF01-544/24-23.3-D	$345
Single Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF526-Di01-22x29	$355
DM-K cube		LDMK	$350
-ZERO set certification		-ZERO	$99

Leica DMi8 (P-Cube) for microscopes: DMi8

LED-YFP-A-LDMP-ZERO (-ZERO pixel set mounted in cube)

$1,359

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
DMi8 (P-Cube) cube		LDMP	$435
-ZERO set certification		-ZERO	$99

Aperio AMF for microscopes: ScanScope FL

LED-YFP-A-AMF-ZERO (-ZERO pixel set mounted in cube)

$1,389

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-509/22-25	$345
Single Band Emitter	25 mm x 3.5 mm	FF01-544/24-25	$345
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF526-Di01-25x36	$355
AMF cube		AMF	$465
-ZERO set certification		-ZERO	$99

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
5-carboxy-2,7-dichlorofluorescein	500	525
5-FAM (5-carboxyfluorescein)	492	518
6-carboxyrhodamine 6G	525	555
6-JOE	520	548
Acridine Orange (+DNA)	502	526
Alexa Fluor^® 488	499	520
Alexa Fluor^® 500	503	525
Alexa Fluor^® 514	518	543
Alexa Fluor^® 532	534	553
ATTO 488	500	525
ATTO 495	495	527
ATTO 520	516	538
ATTO 532	501	545
BODIPY R6G	528	547
Calcein	494	514
Calcium Green-1	507	529
CyQUANT GR-DNA	502	523
DAF-FM-NO	495	519
Dronpa	502	516
DY-505-Phalloidin	503	530
DyLight 488	493	518
Eosin	525	546
FITC (Fluorescein)	495	519
FlAsH-CCPFCC	511	530
Fluo-3	506	527
Fluo-4	494	516
Fluorescein dextran	501	524
Fluorescein-pH 8.0	489	517
Fluoro-Emerald	494	518
FluoSpheres Orange fluorescent microspheres	540	559
FluoSpheres Yellow-Green fluorescent microspheres	503	514
FM 1-43	473	579
Gold 525	521	551
Green 496	496	520
Green 500	501	524
HCS LipidTOX Green phospholipidosis	504	536
HiLyte Fluor™ 488	497	526
JO-JO-1, JO-PRO-1	530	544
LIVE-DEAD Fixable Green Dead Cell Stain	498	525
LysoTracker Yellow HCK-123	488	565
Magnesium Green	507	531
mHoneyDew	478	562
mOrange2	551	565
NeuroTrace 500/525 Green Fluorescent Nissl Stain	495	524
Oregon Green™ 488	498	526
Oregon Green™ 514	513	532
PicoGreen^®	502	522
Qdot^® 525 Nanocrystals	300	525
Qdot^® 545 Nanocrystals	300	543
Rhodamine 110	497	519
Rhodamine 123	507	529
Rhodamine Green	497	523
Rhodol Green	497	524
Sodium Green	507	532
SpectrumGreen	497	538
SYBR Gold nucleic acid gel stain-DNA	496	539
SYBR Green I nucleic acid gel stain-DNA	498	522
SYBR Safe DNA gel stain-DNA	509	526
SYTO 11	506	525
SYTO RNASelect geen fluorescent cell stain	503	527
SYTOX Green-DNA	504	524
TO-PRO-1	515	531
TOTO-1	514	531
TurboYFP	525	538
Venus	516	528
VIC	530	550
Vybrant DyeCycle Green	506	534
Vybrant DyeCycle Orange	519	563
YFP (yellow GFP)	513	530
ZsYellow1	529	539

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?	Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity

Journal Article

Topic	Description
Achieving the Best Alignment for Fluorescent Images	Turan Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005 Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image

White Paper

Topic	Description
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.

LED-YFP-A YFP Venus LED 509/22 544/24 526 Single-Band FF526-Di01 FF01-544/24 FF01-509/22 FF526 Lumencor Sola Spectra-X CoolLED pE-4000 X-Cite Turbo XLED1 110LED-Broadband 120LED-Broadband 6-JOE Alexa-Fluor-514 Alexa514 AF514 Fluo-3 Filter Filters Set Sets LED-YFP-A-000 LED-YFP-A-000-ZERO LED-YFP-A-L01-ZERO LED-YFP-A-U02 LED-YFP-A-NQF LED-YFP-A-NQF-ZERO LED-YFP-A-NTE LED-YFP-A-NTE-ZERO LED-YFP-A-OMF LED-YFP-A-OMF-ZERO LED-YFP-A-OFF LED-YFP-A-OFF-ZERO LED-YFP-A-OFX LED-YFP-A-ZHE LED-YFP-A-ZHE-ZERO LED-YFP-A-LDMP-ZERO LED-YFP-A-LDMK-ZERO LED-YFP-A-AMF-ZERO

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® single-band filter set, optimized for YFP and other like fluorophores, illuminated with LED based light sources

Part Number: LED-YFP-A-000

BrightLine® single-band filter set, optimized for YFP and other like fluorophores, illuminated with LED based light sources

BrightLine^® single-band filter set, optimized for YFP and other like fluorophores, illuminated with LED based light sources

BrightLine^® single-band filter set, optimized for YFP and other like fluorophores, illuminated with LED based light sources