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BrightLine® single-band filter set, optimized for DyLight 405 and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: DyLight405-C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: DyLight405-C

BrightLine® single-band filter set, optimized for DyLight 405 and other like fluorophores

This filter set provides high brightness and contrast for imaging DyLight405TM .

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details DyLight405-C-000 (Standard size set, fits most microscopes2)$910Confirmed AROAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-379/34-25$355
Single Band Emitter25 mm x 3.5 mmFF01-440/40-25$355
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF409-Di03-25x36$292
Click to expand/collapse details DyLight405-C-L01 (Leica 'Large' set)$910Confirmed AROAdd To Cart
Click to expand/collapse details DyLight405-C-U02 (Olympus large-size (FX) set for U-M710I)$1,638Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details DyLight405-C-NQF (set mounted in cube)$1,309Confirmed AROAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details DyLight405-C-NTE (set mounted in cube)$1,309Confirmed AROAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details DyLight405-C-OMF (set mounted in cube)$1,359Confirmed AROAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details DyLight405-C-OFF (set mounted in cube)$1,359Confirmed AROAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details DyLight405-C-OFX (set mounted in cube)$1,609Confirmed AROAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details DyLight405-C-ZHE (set mounted in cube)$1,199Confirmed AROAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, DM-6000, DMI6000 B, DM4 and DM6
Click to expand/collapse details DyLight405-C-LDMK (set mounted in cube)$1,269Confirmed AROAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
7-amino-4-methylcoumarin (AMC)3464421-Star
Alexa Fluor® 3503434411-Star
Alexa Fluor® 4054014223-Star
AMCA (Aminomethylcoumarin)3504483-Star
BD Horizon V4504064491-Star
BFP (EBFP)3804403-Star
Calcofluor White3514441-Star
Cascade Blue™4014193-Star
CellTrace calcein violet4004523-Star
DiFMU-pH 9.03584503-Star
DyLight 3503514341-Star
DyLight 4053984205-Star
FluoSpheres Blue fluorescent microspheres3564141-Star
HiLyte Fluor™ 4054034283-Star
Hoechst 332583524551-Star
LIVE-DEAD Fixable Blue Dead Cell Stain3434411-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034551-Star
LysoSensor Blue3744243-Star
LysoTracker Blue3734223-Star
Marina Blue3634613-Star
Pacific Blue™4044551-Star
Quinine sulfate3494611-Star
Vybrant DyeCycle Violet3694373-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.

DyLight405-C DyLight405 Single-Band FF01-440/40 FF02-379/34 FF409-Di03 FF409 440/40 379/34 409 Filter Filters Set Sets DyLight405-C-000 DyLight405-C-L01 DyLight405-C-U02 DyLight405-C-NQF DyLight405-C-NTE DyLight405-C-OMF DyLight405-C-OFF DyLight405-C-OFX DyLight405-C-ZHE DyLight405-C-LDMK

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