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BrightLine® combination filter set, optimized for DAPI, Spectrum Green, & Spectrum Red and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: DA/SpGr/SpRed-C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: DA/SpGr/SpRed-C

BrightLine® combination filter set, optimized for DAPI, Spectrum Green, & Spectrum Red and other like fluorophores

This combination set offers the flexibility required for simultaneous multi-color blue-green-red FISH analysis. Typically the multiband emission and beamsplitter filters are mounted in a microscope cube, while the excitation filters are mounted in a filter wheel. All of the possible configurations offer high brightness, extremely low crosstalk, and superb signal-to-noise ratio performance.

To simultaneously view three fluorophores, the triple-band exciter (Exciter 5) is used. This configuration is ideal for identifying the nucleus of the cell simultaneously with the green and red markers.

To avoid undesirable ultraviolet (UV) exposure of the sample and the typically dominant brightness of the blue label, it is often desirable to view only the green and red labels. The dual-band exciter is used for this configuration (Exciter 4). Finally, by utilizing individual single-band exciters, one can view or capture a (monochrome) digital image of each label independently (Exciters 1, 2, & 3).

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details DA/SpGr/SpRed-C-000 (Standard size set, fits most microscopes2)$2,265Ships in 2 daysAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-387/11-25$305
Single Band Exciter25 mm x 5.0 mmFF01-494/20-25$405
Single Band Exciter25 mm x 5.0 mmFF03-575/25-25$305
Dual Band Exciter25 mm x 5.0 mmFF01-494/576-25$425
Triple Band Exciter25 mm x 5.0 mmFF01-407/494/576-25$465
Triple Band Emitter25 mm x 3.5 mmFF01-457/530/628-25$465
Triple Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF436/514/604-Di01-25x36$465
Click to expand/collapse details DA/SpGr/SpRed-C-L01 (Leica 'Large' set)$2,265Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details DA/SpGr/SpRed-C-NQF (set mounted in cube)$2,660Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details DA/SpGr/SpRed-C-NTE (set mounted in cube)$2,660Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details DA/SpGr/SpRed-C-OMF (set mounted in cube)$2,700Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details DA/SpGr/SpRed-C-OFF (set mounted in cube)$2,700Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details DA/SpGr/SpRed-C-ZHE (set mounted in cube)$2,540Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details DA/SpGr/SpRed-C-LDMK (set mounted in cube)$2,615Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
DAPI3594613-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
FITC (Fluorescein)4955193-Star
SpectrumGreen4975383-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
SpectrumRed5876153-Star
Texas Red®5926143-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
FISH (Fluorescence in Situ Hybridization)An overview of FISH, including solutions from Semrock and additional learning resources.



DA/SpGr/SpRed-C DAPI SpectrumGreen SpectrumRed SpGreen SpRed DAPI/SpectrumGreen/SpectrumRed DAPI/SpGreen/SpRed FISH FF03-575/25 FF436/514/604 FF436/514/604-Di01 FF01-457/530/628 FF01-494/576 FF01-494/20 FF01-407/494/576 FF01-387/11 575/25 436/514/604 457/530/628 494/576 494/20 407/494/576 387/11 Filter Filters Set Sets DA/SpGr/SpRed-C-000 DA/SpGr/SpRed-C-L01 DA/SpGr/SpRed-C-NQF DA/SpGr/SpRed-C-NTE DA/SpGr/SpRed-C-OMF DA/SpGr/SpRed-C-OFF DA/SpGr/SpRed-C-ZHE DA/SpGr/SpRed-C-LDMK

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