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BrightLine® Sedat filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: DA/FI/TX-3X3M-C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: DA/FI/TX-3X3M-C

BrightLine® Sedat filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

This 7-filter "Sedat" set is based on the award-winning BrightLine blue-green-red multiband filter set, but instead of multiband exciter and emitter filters, it has three single-band exciters, three single-band emitters, and a triple-band beamsplitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore high-speed sequential imaging using a low-noise monochrome camera and filter wheels for both the exciters and the emitters.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details DA/FI/TX-3X3M-C-000 (Standard size set, fits most microscopes2)$2,075Ships in 2 daysAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-387/11-25$305
Single Band Exciter25 mm x 5.0 mmFF01-494/20-25$405
Single Band Exciter25 mm x 5.0 mmFF03-575/25-25$305
Single Band Emitter25 mm x 3.5 mmFF02-447/60-25$305
Single Band Emitter25 mm x 5.0 mmFF02-531/22-25$355
Single Band Emitter25 mm x 3.5 mmFF01-624/40-25$305
Triple Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF436/514/604-Di01-25x36$465
Click to expand/collapse details DA/FI/TX-3X3M-C-L01 (Leica 'Large' set)$2,075Ships in 2 daysAdd To Cart
Click to expand/collapse details DA/FI/TX-3X3M-C-S01 (Sutter threaded set)$2,195Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details DA/FI/TX-3X3M-C-NQF (set mounted in cube)$2,470Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details DA/FI/TX-3X3M-C-NTE (set mounted in cube)$2,470Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details DA/FI/TX-3X3M-C-OMF (set mounted in cube)$2,510Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details DA/FI/TX-3X3M-C-OFF (set mounted in cube)$2,510Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details DA/FI/TX-3X3M-C-ZHE (set mounted in cube)$2,420Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details DA/FI/TX-3X3M-C-LDMK (set mounted in cube)$2,425Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details DA/FI/TX-3X3M-C-LDMP (set mounted in cube)$2,510Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724773-Star
4-methylumbelliferone3574411-Star
7-hydroxy-4-methylcoumarin3604421-Star
Alexa Fluor® 4054014221-Star
AMCA (Aminomethylcoumarin)3504485-Star
ATTO 3903924773-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804403-Star
Calcofluor White3514441-Star
Cascade Blue™4014191-Star
CellTrace calcein violet4004523-Star
DAPI3594615-Star
DEAC4324721-Star
DiFMU-pH 9.03584501-Star
DY-4154184701-Star
DyLight 4053984203-Star
ECFP4354751-Star
HiLyte Fluor™ 4054034281-Star
Hoechst 332583524553-Star
Hoechst 333423524553-Star
Hoechst 345803924403-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
LysoSensor Blue3744243-Star
LysoTracker Blue3734221-Star
Marina Blue3634611-Star
mBBr+GSH3944901-Star
Pacific Blue™4044553-Star
sgBFP™3874513-Star
Sirius3554241-Star
SpectrumBlue4054491-Star
TagBFP4024571-Star
Vybrant DyeCycle Violet3694371-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925183-Star
6-carboxyrhodamine 6G5255551-Star
Acridine Orange (+DNA)5025263-Star
Alexa Fluor® 4884995205-Star
Alexa Fluor® 5005035253-Star
Alexa Fluor® 5145185431-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025283-Star
BODIPY FL5055121-Star
BODIPY FL-MeOH5025111-Star
Calcein4945143-Star
Calcium Green-15075293-Star
CFP24905101-Star
Cy2™4925071-Star
CyQUANT GR-DNA5025233-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915071-Star
DiO4755001-Star
Dronpa5025163-Star
DY-505-Phalloidin5035303-Star
Emerald4915111-Star
FITC (Fluorescein)4955195-Star
FlAsH-CCPFCC5115301-Star
Fluo-35065273-Star
Fluo-44945163-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895173-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035143-Star
GFP (EGFP)4895111-Star
Green 4964965203-Star
Green 5005015243-Star
HCS LipidTOX Green neutral lipid stain4985071-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoTracker Green5015091-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075313-Star
mHoneyDew4785623-Star
MitoTracker™ Green4905121-Star
mWasabi4935091-Star
NBD-X (MeOH)4675381-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135321-Star
PicoGreen®5025223-Star
PKH674885001-Star
Qdot® 525 Nanocrystals3005251-Star
Rhodamine 1104975193-Star
Rhodamine 1235075293-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
Sodium Green5075323-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095263-Star
SYTO 115065253-Star
SYTO 134485061-Star
SYTO 164895203-Star
SYTO 94835001-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboGFP4825031-Star
Venus5165281-Star
Vybrant DyeCycle Green5065343-Star
Vybrant DyeCycle Orange5195631-Star
YFP (yellow GFP)5135301-Star
YO-PRO-14915061-Star
YOYO-14915081-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825951-Star
5-ROX (carboxy-X-rhodamine)5786043-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5685796033-Star
Alexa Fluor® 5945906183-Star
Amplex UltraRed peroxidation product (pH 7.5)5685811-Star
AsRed 25765923-Star
ATTO 5655635921-Star
ATTO 5905946243-Star
ATTO 5946016241-Star
BOBO™-35706053-Star
BODIPY TR-X phallacidin5906213-Star
Calcium Crimson5896093-Star
CellTrace BODIPY TR methyl ester5986251-Star
CellTracker Red CMTPX5866143-Star
Cy3.5™5785911-Star
DsRed5595831-Star
DsRed-Express5565841-Star
dTomato5565821-Star
DY-5905825993-Star
DyLight 5945936183-Star
FluoSpheres Red fluorescent microspheres5776073-Star
HcRed15886181-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846083-Star
HiLyte Fluor™ 5945926163-Star
Hypericin6006031-Star
KFP-Red5755993-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956131-Star
LysoTracker Red5735921-Star
mApple5665941-Star
mCherry5876103-Star
MitoTracker™ Red5785983-Star
mKate25886333-Star
mPlum5896491-Star
mRFP15847003-Star
mRuby5586053-Star
mStrawberry5745961-Star
mTangerine5685851-Star
Nile red-phospolipid5536373-Star
pHrodo™, succinimidyl ester5605871-Star
Propidium Iodide (PI)3056171-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
ReAsH-CCPGCC5926063-Star
Red 5805806033-Star
Resorufin5715841-Star
Rhodamine Red-X5735911-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 6.0)5485861-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 6.0)5495871-Star
SNARF-1 514nm (ph 9.0)5766383-Star
SpectrumOrange5545871-Star
SpectrumRed5876151-Star
Sulforhodamine 101-EtOH5785931-Star
SYTO 176196381-Star
Texas Red dextran5926133-Star
Texas Red DHPE5846083-Star
Texas Red®5926141-Star
TurboFP635(Katushka)5916383-Star
X-Rhod-1 Indicator5806013-Star
YO-PRO-36136291-Star
YOYO-36116311-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



DA/FI/TX-3X3M-C Sedat Triple-Band DAPI FITC TexasRed TxRed DAPI/FITC/TexasRed DAPI/FITC/TxRed DA/FI/TX FF02-531/22 FF03-575/25 FF436/514/604-Di01 FF01-494/20 FF01-387/11 FF02-447/60 FF01-624/40 531/22 575/25 FF436/514/604 436/514/604 494/20 387/11 447/60 624/40 Filter Filters Set Sets Triple 3X3M DA/FI/TX-3X3M-C-000 DA/FI/TX-3X3M-C-L01 DA/FI/TX-3X3M-C-S01 DA/FI/TX-3X3M-C-NQF DA/FI/TX-3X3M-C-NTE DA/FI/TX-3X3M-C-OMF DA/FI/TX-3X3M-C-OFF DA/FI/TX-3X3M-C-ZHE DA/FI/TX-3X3M-C-LDMP DA/FI/TX-3X3M-C-LDMK

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