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BrightLine^® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: DA/FI/TX-3X-C-000

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Filters and Pricing
Specifications
Fluorophore
Technical Info

BrightLine^® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

This 5-filter "Pinkel" set is based on the award-winning BrightLine blue-green-red multiband filter set, but instead of a multiband exciter it has three single-band exciters, a multiband beamsplitter, and a multiband emitter. With its high brightness, extremely low crosstalk, and superb signal-to-noise performance, it is ideal for three-fluorophore imaging using a low-noise monochrome camera.

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

Part Number

Price¹

Stock Status

Quantity

DA/FI/TX-3X-C-000 (Standard size set, fits most microscopes²)

$1,655

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-457/530/628-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF436/514/604-Di01-25x36	$465

DA/FI/TX-3X-C-L01 (Leica 'Large' set)

$1,655

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	23.3 mm x 2.0 mm	FF01-457/530/628-23.3-D	$465
Triple Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF436/514/604-Di01-22x29	$465

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325

DA/FI/TX-3X-C-S01 (Sutter threaded set)

$1,715

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-387/11-25-STR	$345
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF01-494/20-25-STR	$425
Single Band Exciter	25 mm threaded ring for Sutter Lambda filter wheel	FF03-575/25-25-STR	$345
Triple Band Emitter	25 mm x 3.5 mm	FF01-457/530/628-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF436/514/604-Di01-25x36	$465

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:

These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.

Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

DA/FI/TX-3X-C-NQF (set mounted in cube)

$2,050

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-457/530/628-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF436/514/604-Di01-25x36	$465
Quadfluor cube		NQF	$395

Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

DA/FI/TX-3X-C-NTE (set mounted in cube)

$2,050

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-457/530/628-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF436/514/604-Di01-25x36	$465
TE 2000 cube		NTE	$395

Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

DA/FI/TX-3X-C-OMF (set mounted in cube)

$2,090

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-457/530/628-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF436/514/604-Di01-25x36	$465
U-MF2 cube		OMF	$435

Olympus U-FF for microscopes: BX53, BX63

DA/FI/TX-3X-C-OFF (set mounted in cube)

$2,090

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-457/530/628-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF436/514/604-Di01-25x36	$465
U-FF cube		OFF	$435

Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

DA/FI/TX-3X-C-ZHE (set mounted in cube)

$1,930

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-457/530/628-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF436/514/604-Di01-25x36	$465
FL Cube EC P+C cube		ZHE	$275

Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B

DA/FI/TX-3X-C-LDMK (set mounted in cube)

$2,005

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	23.3 mm x 2.0 mm	FF01-457/530/628-23.3-D	$465
Triple Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF436/514/604-Di01-22x29	$465
DM-K cube		LDMK	$350

Leica DMi8 (P-Cube) for microscopes: DMi8

DA/FI/TX-3X-C-LDMP (set mounted in cube)

$2,090

Confirmed ARO

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF01-387/11-25	$325
Single Band Exciter	25 mm x 5.0 mm	FF01-494/20-25	$405
Single Band Exciter	25 mm x 5.0 mm	FF03-575/25-25	$325
Triple Band Emitter	25 mm x 3.5 mm	FF01-457/530/628-25	$465
Triple Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF436/514/604-Di01-25x36	$465
DMi8 (P-Cube) cube		LDMP	$435

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Blue

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
1,8-ANS	372	477
4-methylumbelliferone	357	441
7-hydroxy-4-methylcoumarin	360	442
Alexa Fluor^® 405	401	422
AMCA (Aminomethylcoumarin)	350	448
ATTO 390	392	477
BD Horizon V450	406	449
BFP (EBFP)	380	440
Calcofluor White	351	444
Cascade Blue™	401	419
CellTrace calcein violet	400	452
DAPI	359	461
DiFMU-pH 9.0	358	450
DyLight 405	398	420
ER-Tracker™ Blue-White DPX	372	557
HiLyte Fluor™ 405	403	428
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
Marina Blue	363	461
mBBr+GSH	394	490
Pacific Blue™	404	455
sgBFP™	387	451
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Green

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
5-carboxy-2,7-dichlorofluorescein	500	525
5-FAM (5-carboxyfluorescein)	492	518
6-carboxyrhodamine 6G	525	555
Acridine Orange (+DNA)	502	526
Alexa Fluor^® 488	499	520
Alexa Fluor^® 500	503	525
Alexa Fluor^® 514	518	543
ATTO 488	500	525
ATTO 495	495	527
BCECF (pH 5.5)	481	518
BCECF (pH 9.0)	502	528
BODIPY FL	505	512
BODIPY FL-MeOH	502	511
Calcein	494	514
Calcium Green-1	507	529
CFP2	490	510
Cy2™	492	507
CyQUANT GR-DNA	502	523
DAF-FM-NO	495	519
Dendra2 (Green)	491	507
DiO	475	500
Dronpa	502	516
DY-505-Phalloidin	503	530
Emerald	491	511
FITC (Fluorescein)	495	519
FlAsH-CCPFCC	511	530
Fluo-3	506	527
Fluo-4	494	516
Fluorescein dextran	501	524
Fluorescein-pH 8.0	489	517
Fluoro-Emerald	494	518
FluoSpheres Yellow-Green fluorescent microspheres	503	514
GFP (EGFP)	489	511
Green 496	496	520
Green 500	501	524
HCS LipidTOX Green neutral lipid stain	498	507
HCS LipidTOX Green phospholipidosis	504	536
HiLyte Fluor™ 488	497	526
LIVE-DEAD Fixable Green Dead Cell Stain	498	525
LysoTracker Green	501	509
LysoTracker Yellow HCK-123	488	565
Magnesium Green	507	531
mHoneyDew	478	562
MitoTracker™ Green	490	512
mWasabi	493	509
NBD-X (MeOH)	467	538
NeuroTrace 500/525 Green Fluorescent Nissl Stain	495	524
Oregon Green™ 488	498	526
Oregon Green™ 514	513	532
PicoGreen^®	502	522
PKH67	488	500
Qdot^® 525 Nanocrystals	300	525
Rhodamine 110	497	519
Rhodamine 123	507	529
Rhodamine Green	497	523
Rhodol Green	497	524
Sodium Green	507	532
SpectrumGreen	497	538
SYBR Gold nucleic acid gel stain-DNA	496	539
SYBR Green I nucleic acid gel stain-DNA	498	522
SYBR Safe DNA gel stain-DNA	509	526
SYTO 11	506	525
SYTO 13	448	506
SYTO 16	489	520
SYTO 9	483	500
SYTO RNASelect geen fluorescent cell stain	503	527
SYTOX Green-DNA	504	524
TO-PRO-1	515	531
TOTO-1	514	531
TurboGFP	482	503
Venus	516	528
Vybrant DyeCycle Green	506	534
Vybrant DyeCycle Orange	519	563
YFP (yellow GFP)	513	530
YO-PRO-1	491	506
YOYO-1	491	508

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Red

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
2-dodecylresorufin-lipid	582	595
5-ROX (carboxy-X-rhodamine)	578	604
7-AAD (7-aminoactinomycin D)	548	648
Alexa Fluor^® 568	579	603
Alexa Fluor^® 594	590	618
AsRed 2	576	592
ATTO 565	563	592
ATTO 590	594	624
ATTO 594	601	624
BOBO™-3	570	605
BODIPY TR-X phallacidin	590	621
Calcium Crimson	589	609
CellTrace BODIPY TR methyl ester	598	625
CellTracker Red CMTPX	586	614
Cy3.5™	578	591
DsRed	559	583
dTomato	556	582
DY-590	582	599
DyLight 594	593	618
FluoSpheres Red fluorescent microspheres	577	607
HcRed1	588	618
HCS LipidTOX Red neutral lipid stain	582	616
HCS LipidTOX Red phospholipidosis	584	608
HiLyte Fluor™ 594	592	616
KFP-Red	575	599
LIVE-DEAD Fixable Red Dead Cell Stain	595	613
LysoTracker Red	573	592
mApple	566	594
mCherry	587	610
MitoTracker™ Red	578	598
mKate2	588	633
mPlum	589	649
mRFP1	584	700
mRuby	558	605
mStrawberry	574	596
mTangerine	568	585
Nile red-phospolipid	553	637
pHrodo™, succinimidyl ester	560	587
Propidium Iodide (PI)	305	617
Qdot^® 625 Nanocrystals	300	621
ReAsH-CCPGCC	592	606
Red 580	580	603
Rhodamine Red-X	573	591
SNARF (carboxy) 514 Excitation pH9	576	638
SNARF-1 488nm (ph 6.0)	548	586
SNARF-1 488nm (ph 9.0)	576	638
SNARF-1 514nm (ph 6.0)	549	587
SNARF-1 514nm (ph 9.0)	576	638
SpectrumRed	587	615
SYTO 17	619	638
Texas Red dextran	592	613
Texas Red DHPE	584	608
Texas Red^®	592	614
TurboFP635(Katushka)	591	638
X-Rhod-1 Indicator	580	601
YO-PRO-3	613	629
YOYO-3	611	631

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set Terminology	Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets

Journal Article

Topic	Description
New Optical Filters Improve High-Speed Multicolor Fluorescence Imaging	Turan Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006 Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging

White Paper

Topic	Description
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.

DA/FI/TX-3X-C DAPI FITC TexasRed TxRed DAPI/FITC/TxRed DAPI/FITC/TexasRed DA/FI/TX DA/FI/TXRED Pinkel Triple-Band FF01-387/11 FF01-457/530/628 FF01-494/20 FF03-575/25 FF436/514/604-Di01 FF436/514/604 387/11 457/530/628 494/20 575/25 436/514/604 Filter Filters Set Sets Triple 3X DA/FI/TX-3X-C-000 DA/FI/TX-3X-C-L01 DA/FI/TX-3X-C-S01 DA/FI/TX-3X-C-NQF DA/FI/TX-3X-C-NTE DA/FI/TX-3X-C-OMF DA/FI/TX-3X-C-OFF DA/FI/TX-3X-C-ZHE DA/FI/TX-3X-C-LDMP DA/FI/TX-3X-C-LDMK

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

Part Number: DA/FI/TX-3X-C-000

BrightLine® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

BrightLine^® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores

BrightLine^® Pinkel filter set, optimized for DAPI, FITC, & Texas Red and other like fluorophores