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BrightLine® full-multiband filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines

Semrock Set / Fluorophore Graphing

Part Number: LED-DA/FI/TR/Cy5-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 4054014225-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804405-Star
Cascade Blue™4014195-Star
CellTrace calcein violet4004521-Star
DAPI3594615-Star
DyLight 4053984205-Star
HiLyte Fluor™ 4054034285-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034551-Star
LysoSensor Blue3744243-Star
Marina Blue3634611-Star
Pacific Blue™4044551-Star
sgBFP™3874515-Star
SpectrumBlue4054493-Star
TagBFP4024571-Star
Vybrant DyeCycle Violet3694371-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-FAM (5-carboxyfluorescein)4925183-Star
Acridine Orange (+DNA)5025261-Star
Acridine Yellow4705501-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995205-Star
Alexa Fluor® 5005035251-Star
AmCyan14584893-Star
ATTO 4654535071-Star
ATTO 4885005255-Star
ATTO 4954955271-Star
BOBO™-14614841-Star
BODIPY FL5055121-Star
BODIPY FL-MeOH5025111-Star
Calcein4945143-Star
CFP24905105-Star
Cy2™4925075-Star
CyQUANT GR-DNA5025231-Star
DAF-FM-NO4955191-Star
Dendra2 (Green)4915075-Star
DiO4755005-Star
Dronpa5025161-Star
DY-505-Phalloidin5035301-Star
ecliptic pHluorin pH5.54735075-Star
Emerald4915113-Star
evoglow-Bs14494961-Star
FITC (Fluorescein)4955195-Star
Fluo-44945163-Star
Fluorescein dextran5015241-Star
Fluorescein-pH 8.04895171-Star
Fluoro-Emerald4945181-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
GFP (EGFP)4895115-Star
Green 4964965201-Star
Green 5005015241-Star
HiLyte Fluor™ 4884975261-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985251-Star
LysoSensor Green4485021-Star
LysoTracker Green5015091-Star
LysoTracker Yellow HCK-1234885651-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905123-Star
mTFP14624921-Star
mWasabi4935093-Star
NBD-X (MeOH)4675381-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
Oregon Green™ 4884985261-Star
PicoGreen®5025221-Star
PKH674885003-Star
Qdot® 525 Nanocrystals3005251-Star
Rhodamine 1104975191-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
sgGFP™ (super glow GFP)4725065-Star
Sodium Green5075321-Star
SpectrumGreen4975381-Star
SYBR Gold nucleic acid gel stain-DNA4965391-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895205-Star
SYTO 94835005-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
TagCFP4584801-Star
TOTO-15145311-Star
TurboGFP4825035-Star
wtGFP (wild type GFP, non-UV excitation)4745095-Star
YO-PRO-14915063-Star
YOYO-14915083-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Orange
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825953-Star
5-ROX (carboxy-X-rhodamine)5786041-Star
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425683-Star
Alexa Fluor® 5465565723-Star
Alexa Fluor® 5555535651-Star
Alexa Fluor® 5685796033-Star
Amplex UltraRed peroxidation product (pH 7.5)5685813-Star
AsRed 25765921-Star
ATTO 5505535763-Star
ATTO 5655635925-Star
BOBO™-35706053-Star
BODIPY TMR-X5445731-Star
BODIPY TR-X (MeOH)5445731-Star
Calcium Crimson5896091-Star
Calcium Orange5896093-Star
CellTracker Red CMTPX5866141-Star
Cy3.5™5785911-Star
Cy3™5545661-Star
Dendra2 (Red)5545691-Star
DiI5515691-Star
DsRed5595835-Star
DsRed-Express5565845-Star
dTomato5565825-Star
DY-5905825991-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276171-Star
FluoSpheres Red fluorescent microspheres5776071-Star
Gold 5505515641-Star
HCS LipidTOX Red neutral lipid stain5826161-Star
HCS LipidTOX Red phospholipidosis5846081-Star
HiLyte Fluor™ 5555505661-Star
KFP-Red5755991-Star
LOLO-15685803-Star
LysoTracker Red5735921-Star
Magnesium Orange5505743-Star
mApple5665945-Star
mCherry5876101-Star
Merocyanine 5405595793-Star
MitoTracker™ Orange5515753-Star
MitoTracker™ Red5785981-Star
mOrange5465621-Star
mRFP5555835-Star
mRFP15847001-Star
mRuby5586055-Star
mStrawberry5745961-Star
mTangerine5685855-Star
Nile red-triglyceride5105831-Star
OFP5475671-Star
pHrodo™, succinimidyl ester5605875-Star
POPO-35335741-Star
PO-PRO-35395701-Star
Propidium Iodide (PI)3056171-Star
Pro-Q Diamond5565835-Star
Qdot® 585 Nanocrystals3005881-Star
Qdot® 605 Nanocrystals3006021-Star
ReAsH-CCPGCC5926061-Star
Red 5805806031-Star
Resorufin5715843-Star
Rhod-25535773-Star
Rhodamine phalloidin5585753-Star
Rhodamine Red-X5735911-Star
R-phycoerythrin (PE)5655763-Star
SNARF-1 488nm (ph 6.0)5485865-Star
SNARF-1 488nm (ph 9.0)5766381-Star
SNARF-1 514nm (ph 6.0)5495875-Star
SpectrumOrange5545875-Star
Sulforhodamine 101-EtOH5785931-Star
SYTO 825405591-Star
SYTOX Orange5475701-Star
Tetramethylrhodamine dextran5545825-Star
Texas Red DHPE5846081-Star
TRITC (Tetramethylrhodamine)5525785-Star
TurboRFP5535731-Star
X-Rhod-1 Indicator5806011-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556151-Star
Alexa Fluor® 6336316473-Star
Alexa Fluor® 6476536685-Star
Alexa Fluor® 6606636911-Star
Alexa Fluor® 6806797021-Star
Alexa Fluor® 7006967191-Star
APC6516603-Star
APC (Allophycocyanin)6516605-Star
ATTO 6336296583-Star
ATTO 6356356583-Star
ATTO 6476446705-Star
ATTO 647N6456683-Star
ATTO 6556636831-Star
ATTO 6806807001-Star
ATTO 7006997191-Star
BODIPY 650/665-X6466645-Star
Carboxynaphthofluorescein5996741-Star
Cy5.5™6726901-Star
Cy5™6496665-Star
DDAO6486575-Star
DiD (DiIC18(5)) - Lipophilic Tracer6486705-Star
DyLight 6496516733-Star
DyLight 6806767051-Star
FluoSpheres Dark Red fluorescent microspheres6566831-Star
HCS CellMask Red cytoplasmic6226441-Star
HCS LipidTOX Deep Red neutral lipid stain6356521-Star
HiLyte Fluor™ 6476496735-Star
LDS 7515607111-Star
LIVE-DEAD Fixable Far Red Dead Cell Stain6536681-Star
MitoTracker™ Deep Red6416625-Star
Nile blue-EtOH6316605-Star
Qdot® 705 Nanocrystals3007021-Star
Qdot® 800 Nanocrystals3007921-Star
Quasar® 7056887061-Star
Red 6506496655-Star
SpectrumFRed (Far Red)6506735-Star
SYTO 596226441-Star
SYTO 606506813-Star
SYTO 616186501-Star
SYTOX Red-DNA6406585-Star
TO-PRO-36426573-Star
TOTO-36426615-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
Revised Filter Set

LED-DA_FI_TR_Cy5-B.png

BrightLine® full-multiband filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines

Multiband set with quad-band exciter, emitter, and beamsplitter filters. Ideal for use with: Blue: DAPI; Green: FITC , GFP ; Orange: TRITC ; Red: Cy5
Part # LED-DA/FI/TR/Cy5-B-000
$1375
(Non-US pricing info)


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US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



LED-DA/FI/TR/Cy5-A

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