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BrightLine® long-pass laser filter set, optimized for 561 & 568 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF561/LP-C-000

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Semrock Filter Set: LF561/LP-C

BrightLine® long-pass laser filter set, optimized for 561 & 568 nm laser sources

This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from RFP (e.g., mCherry), Texas Red, and other similar fluorophores while offering superb blocking of popular 561 nm (solid-state) or 568 nm (Kr-ion gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF561/LP-D


Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LF561/LP-C-000 (Standard size set, fits most microscopes2)$915In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-561/14-25$305
Long Pass Emitter25 mm x 3.5 mmBLP02-561R-25$355
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmDi02-R561-25x36$405
Click to expand/collapse details LF561/LP-C-L01 (Leica 'Large' set)$915Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LF561/LP-C-NQF (set mounted in cube)$1,310In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LF561/LP-C-NTE (set mounted in cube)$1,310In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LF561/LP-C-OMF (set mounted in cube)$1,350In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LF561/LP-C-OFF (set mounted in cube)$1,350In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LF561/LP-C-ZHE (set mounted in cube)$1,190In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LF561/LP-C-LDMK (set mounted in cube)$1,265Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LF561/LP-C-LDMP (set mounted in cube)$1,350In StockAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825953-Star
5-carboxynapthofluorescein (pH 10)5556153-Star
5-ROX (carboxy-X-rhodamine)5786043-Star
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425681-Star
7-AAD (7-aminoactinomycin D)5486483-Star
Alexa Fluor® 5465565721-Star
Alexa Fluor® 5685796035-Star
Alexa Fluor® 5945906181-Star
Amplex UltraRed peroxidation product (pH 7.5)5685813-Star
AsRed 25765923-Star
ATTO 5505535761-Star
ATTO 5655635923-Star
ATTO 5905946241-Star
BOBO™-35706053-Star
BODIPY TR-X phallacidin5906213-Star
Calcium Crimson5896091-Star
Carboxynaphthofluorescein5996743-Star
CellTrace BODIPY TR methyl ester5986251-Star
CellTracker Red CMTPX5866143-Star
ChlorophyllA4806501-Star
Cy3.5™5785913-Star
Cy3™5545663-Star
DDAO6486573-Star
Dendra2 (Red)5545691-Star
DsRed5595835-Star
DsRed-Express5565843-Star
dTomato5565823-Star
DY-5905825993-Star
DyLight 5945936181-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276173-Star
FluoSpheres Red fluorescent microspheres5776073-Star
FM 4-645057253-Star
HcRed15886181-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846083-Star
HiLyte Fluor™ 5945926161-Star
Hypericin6006031-Star
KFP-Red5755993-Star
LDS 7515607113-Star
LOLO-15685803-Star
LysoTracker Red5735923-Star
Magnesium Orange5505741-Star
mApple5665943-Star
mCherry5876105-Star
Merocyanine 5405595793-Star
MitoTracker™ Orange5515751-Star
MitoTracker™ Red5785983-Star
mKate25886333-Star
mPlum5896493-Star
mRFP5555833-Star
mRFP15847005-Star
mRuby5586053-Star
mStrawberry5745963-Star
mTangerine5685853-Star
Nile red-phospolipid5536373-Star
Orange 5525525741-Star
PerCP4906773-Star
pHrodo™, succinimidyl ester5605873-Star
Propidium Iodide (PI)3056173-Star
Pro-Q Diamond5565833-Star
Qdot® 585 Nanocrystals3005883-Star
Qdot® 605 Nanocrystals3006023-Star
Qdot® 625 Nanocrystals3006211-Star
Qdot® 655 Nanocrystals3006543-Star
Qdot® 705 Nanocrystals3007023-Star
Qdot® 800 Nanocrystals3007923-Star
ReAsH-CCPGCC5926063-Star
Red 5805806033-Star
Resorufin5715843-Star
Rhod-25535771-Star
Rhodamine phalloidin5585751-Star
Rhodamine Red-X5735913-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 6.0)5485863-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 6.0)5495873-Star
SNARF-1 514nm (ph 9.0)5766383-Star
SpectrumOrange5545873-Star
Sulforhodamine 101-EtOH5785933-Star
SYPRO Ruby protein gel stain4696311-Star
Tetramethylrhodamine dextran5545823-Star
Texas Red dextran5926131-Star
Texas Red DHPE5846083-Star
Texas Red®5926143-Star
TRITC (Tetramethylrhodamine)5525783-Star
TurboFP635(Katushka)5916383-Star
TurboRFP5535731-Star
X-Rhod-1 Indicator5806013-Star
YOYO-36116311-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
Revised Filter Set

LF561_LP-D.png

BrightLine® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 561 & 568 nm laser sources

1λ P-V RWE super-resolution laser optimized long-pass filter set. Ideal for use with: mRFP1, mCherry, Texas Red, Alexa Fluor 568, DsRed, HcRed1, Rhodamine, Cy3, mApple
Part # LF561/LP-D-000
$965
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



LF561/LP-C 561/LP Long-Pass-Filter-Set Laser-Set Di02-R561 BLP02-561R FF01-561/14 561/14 561R R561 Filter Filters Set Sets LF561/LP-C-000 LF561/LP-C-L01 LF561/LP-C-NQF LF561/LP-C-NTE LF561/LP-C-OMF LF561/LP-C-OFF LF561/LP-C-ZHE LF561/LP-C-LDMP LF561/LP-C-LDMK

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