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BrightLine® single-band laser filter set, optimized for 635 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF635-B-000

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Semrock Filter Set: LF635-B

BrightLine® single-band laser filter set, optimized for 635 nm laser sources

This set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from Cy5 and other similar fluorophores while offering superb blocking of popular 635 nm (solid-state laser diode), 633 nm (HeNe gas) or 647 nm (Kr-ion gas) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF635-C


Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LF635-B-000 (Standard size set, fits most microscopes2)$915Ships in 2 daysAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-640/14-25$355
Single Band Emitter25 mm x 3.5 mmFF01-676/29-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmDi02-R635-25x36$405
Click to expand/collapse details LF635-B-L01 (Leica 'Large' set)$915Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LF635-B-NQF (set mounted in cube)$1,310Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LF635-B-NTE (set mounted in cube)$1,310Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LF635-B-OMF (set mounted in cube)$1,350Contact UsAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LF635-B-OFF (set mounted in cube)$1,350Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LF635-B-ZHE (set mounted in cube)$1,190Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LF635-B-LDMK (set mounted in cube)$1,265Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LF635-B-LDMP (set mounted in cube)$1,350Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556151-Star
Alexa Fluor® 6336316475-Star
Alexa Fluor® 6356336481-Star
Alexa Fluor® 6476536685-Star
Alexa Fluor® 6606636913-Star
APC6516603-Star
APC (Allophycocyanin)6516605-Star
ATTO 6336296583-Star
ATTO 6356356583-Star
ATTO 6476446703-Star
ATTO 647N6456683-Star
ATTO 6556636833-Star
BODIPY 650/665-X6466645-Star
Carboxynaphthofluorescein5996741-Star
Cy5.5™6726901-Star
Cy5™6496665-Star
DDAO6486573-Star
DiD (DiIC18(5)) - Lipophilic Tracer6486705-Star
DY-6306346563-Star
DyLight 6336206491-Star
DyLight 6496516733-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Dark Red fluorescent microspheres6566833-Star
HCS CellMask Red cytoplasmic6226441-Star
HCS LipidTOX Deep Red neutral lipid stain6356521-Star
HiLyte Fluor™ 6476496733-Star
LIVE-DEAD Fixable Far Red Dead Cell Stain6536683-Star
MitoTracker™ Deep Red6416623-Star
Nile blue-EtOH6316603-Star
Qdot® 705 Nanocrystals3007021-Star
Red 6506496653-Star
SpectrumFRed (Far Red)6506733-Star
SYTO 596226441-Star
SYTO 606506813-Star
SYTO 616186501-Star
SYTOX Red-DNA6406583-Star
TO-PRO-36426573-Star
TOTO-36426613-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
Revised Filter Set

LF635-C.png

BrightLine® single-band 1λ P-V RWE super-resolution laser filter set, optimized for 635 nm laser sources

1λ P-V RWE super-resolution laser optimized single-band filter set. Ideal for use with: Cy5, Alexa Fluor® 647, APC, ATTO 647, BODIPY 650/665-X, MitoTracker™ Deep Red
Part # LF635-C-000
$965
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



LF635-B 635 Laser-Set Single-Band Di02-R635 FF01-676/29 FF01-640/14 640/14 676/29 R635 Filter Filters Set Sets LF635-B-000 LF635-B-L01 LF635-B-NQF LF635-B-NTE LF635-B-OMF LF635-B-OFF LF635-B-ZHE LF635-B-LDMP LF635-B-LDMK

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