Welcome, Guest: Login or Register My Cart (0)

BrightLine® long-pass laser filter set, optimized for 488 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF488/LP-C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: LF488/LP-C

BrightLine® long-pass laser filter set, optimized for 488 nm laser sources

This long-pass set is designed to take advantage of the superior resolution, sensitivity, and image fidelity of today's state-of-the-art laser-based microscopes. The filter spectra are optimized to capture the maximum fluorescence from GFP, FITC, and other similar fluorophores while offering superb blocking of popular 473 nm (solid-state) or 488 nm (Ar-ion gas and solid-state) lasers. The dichroic offers excellent flatness for minimal focus shift and aberrations of the laser beam spot at the sample.

Our improved version is certified to deliver dichroic flatness <= 1λ P-V RWE @ 632.8 nm for loose and cube mounted [NTE, OFF, OMF or ZHE] filter sets LF488/LP-D


Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details LF488/LP-C-000 (Standard size set, fits most microscopes2)$915In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-482/18-25$305
Long Pass Emitter25 mm x 3.5 mmBLP01-488R-25$355
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmDi02-R488-25x36$405
Click to expand/collapse details LF488/LP-C-L01 (Leica 'Large' set)$915Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details LF488/LP-C-NQF (set mounted in cube)$1,310In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details LF488/LP-C-NTE (set mounted in cube)$1,310In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details LF488/LP-C-OMF (set mounted in cube)$1,350In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details LF488/LP-C-OFF (set mounted in cube)$1,350In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details LF488/LP-C-ZHE (set mounted in cube)$1,190In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details LF488/LP-C-LDMK (set mounted in cube)$1,265Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details LF488/LP-C-LDMP (set mounted in cube)$1,350In StockAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925183-Star
6-carboxyrhodamine 6G5255551-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Acridine Orange (+DNA)5025263-Star
Acridine Orange (+RNA)4606503-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995205-Star
Alexa Fluor® 5005035253-Star
AmCyan14584891-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025283-Star
BODIPY FL5055123-Star
BODIPY FL-MeOH5025113-Star
Calcein4945145-Star
Calcium Green-15075291-Star
CFP24905103-Star
Cy2™4925075-Star
CyQUANT GR-DNA5025233-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915073-Star
Dendra2 (Red)5545691-Star
Di-8-ANEPPS4676313-Star
DiA4575863-Star
DiO4755003-Star
Dronpa5025163-Star
DsRed-Express5565843-Star
DY-505-Phalloidin5035303-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915113-Star
Ethidium bromide5186033-Star
Ethidium homodimer5276173-Star
FITC (Fluorescein)4955195-Star
FlAsH-CCPFCC5115301-Star
Fluo-35065271-Star
Fluo-44945163-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895173-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035143-Star
FM 1-434735793-Star
FM 4-645057253-Star
GFP (EGFP)4895115-Star
Green 4964965203-Star
Green 5005015243-Star
HCS LipidTOX Green neutral lipid stain4985073-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975263-Star
JC-14995253-Star
LDS 7515607111-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoSensor Green4485023-Star
LysoTracker Green5015093-Star
LysoTracker Yellow HCK-1234885653-Star
Magnesium Green5075311-Star
mHoneyDew4785623-Star
MitoTracker™ Green4905125-Star
mWasabi4935093-Star
NBD-X (MeOH)4675383-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Nile red-triglyceride5105833-Star
OFP5475671-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135321-Star
PerCP4906773-Star
PicoGreen®5025223-Star
PKH674885003-Star
Propidium Iodide (PI)3056171-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
Qdot® 565 Nanocrystals3005641-Star
Qdot® 585 Nanocrystals3005881-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Qdot® 655 Nanocrystals3006543-Star
Qdot® 705 Nanocrystals3007023-Star
Qdot® 800 Nanocrystals3007921-Star
ratiometric pHluorin pH55005093-Star
Rhodamine 1104975193-Star
Rhodamine 1235075291-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
R-phycoerythrin (PE)5655761-Star
rsGFP (red shifted GFP, S65T)4985163-Star
sgGFP™ (super glow GFP)4725065-Star
SNARF-1 488nm (ph 6.0)5485861-Star
SNARF-1 514nm (ph 6.0)5495871-Star
Sodium Green5075323-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYPRO Ruby protein gel stain4696313-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895203-Star
SYTO 94835003-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboGFP4825033-Star
Vybrant DyeCycle Green5065343-Star
Vybrant DyeCycle Orange5195631-Star
wtGFP (wild type GFP, non-UV excitation)4745095-Star
YO-PRO-14915063-Star
YOYO-14915083-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Flatness of Dichroic Beamsplitters Affects Focus and Image QualityAnalysis of how the radius of curvature of a dichroic filter affects focal plane shift, spot size and image fidelity in fluorescence microscopy.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.
Revised Filter Set

LF488_LP-D.png

BrightLine® long-pass 1λ P-V RWE super-resolution laser filter set, optimized for 488 nm laser sources

1λ P-V RWE super-resolution laser optimized long-pass filter set. Ideal for use with: GFP , FITC , Alexa Fluor 488, sgGFP , wtGFP , rsGFP , Cy2, BODIPY, Calcein, MitoTracker Green
Part # LF488/LP-D-000
$965
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



LF488/LP-C 488/LP Long-Pass-Filter-Set Laser-Set FF02-482/18 BLP01-488R Di02-R488 482/18 R488 488R Filter Filters Set Sets LF488/LP-C-000 LF488/LP-C-L01 LF488/LP-C-NQF LF488/LP-C-NTE LF488/LP-C-OMF LF488/LP-C-OFF LF488/LP-C-ZHE LF488/LP-C-LDMP LF488/LP-C-LDMK

The item has been added to your shopping cart.

The item has been added to your shopping cart, however, your IP address appears to be outside the US/ Canada, so online/ direct orders from Semrock are not allowed. Please see the International Distributor page to find your non-US distributor.

We apologize for any inconvenience.

The shopping cart is offline. Please call toll-free 1-866-SEMROCK (866-736-7625) or email us to place your order.