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BrightLine® Pinkel laser filter set, optimized for 405, 488, 543, & 635 nm laser sources

Semrock Set / Fluorophore Graphing

Part Number: LF405/488/543/635-4X-A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Single Band Exciter 1FF01-390/40
Single Band Exciter 2FF02-482/18
Single Band Exciter 3FF01-543/3
Single Band Exciter 4FF01-640/14
Quad Band EmitterFF01-446/515/588/700
Quad Band DichroicDi01-R405/488/543/635

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724771-Star
4-methylumbelliferone3574411-Star
7-hydroxy-4-methylcoumarin3604423-Star
Alexa Fluor® 4054014221-Star
AMCA (Aminomethylcoumarin)3504483-Star
ATTO 3903924771-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804405-Star
Calcofluor White3514441-Star
Cascade Blue™4014191-Star
CellTrace calcein violet4004523-Star
DAPI3594615-Star
DiFMU-pH 9.03584501-Star
DY-4154184701-Star
DyLight 4053984203-Star
ER-Tracker™ Blue-White DPX3725571-Star
HiLyte Fluor™ 4054034283-Star
Hoechst 332583524551-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
LysoSensor Blue3744243-Star
LysoTracker Blue3734221-Star
Marina Blue3634613-Star
Pacific Blue™4044553-Star
sgBFP™3874513-Star
Sirius3554243-Star
SpectrumBlue4054493-Star
TagBFP4024573-Star
Vybrant DyeCycle Violet3694373-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925183-Star
Acridine Orange (+DNA)5025263-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995203-Star
Alexa Fluor® 5005035253-Star
AmCyan14584893-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025283-Star
BODIPY FL5055123-Star
BODIPY FL-MeOH5025113-Star
Calcein4945143-Star
Calcium Green-15075291-Star
CFP24905103-Star
Cy2™4925073-Star
CyQUANT GR-DNA5025233-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915073-Star
DiO4755003-Star
Dronpa5025163-Star
DY-505-Phalloidin5035303-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915113-Star
evoglow-Bs14494961-Star
FITC (Fluorescein)4955195-Star
FlAsH-CCPFCC5115301-Star
Fluo-35065271-Star
Fluo-44945163-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895173-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035143-Star
FM 1-434735791-Star
GFP (EGFP)4895115-Star
Green 4964965203-Star
Green 5005015243-Star
HCS LipidTOX Green neutral lipid stain4985073-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoSensor Green4485023-Star
LysoTracker Green5015093-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075311-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905123-Star
mWasabi4935093-Star
NBD-X (MeOH)4675383-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135321-Star
PicoGreen®5025223-Star
PKH674885003-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
ratiometric pHluorin pH55005093-Star
Rhodamine 1104975193-Star
Rhodamine 1235075291-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
sgGFP™ (super glow GFP)4725063-Star
Sodium Green5075323-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895203-Star
SYTO 94835003-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboGFP4825033-Star
Vybrant DyeCycle Green5065343-Star
wtGFP (wild type GFP, non-UV excitation)4745093-Star
YO-PRO-14915063-Star
YOYO-14915083-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Orange
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825953-Star
5-ROX (carboxy-X-rhodamine)5786043-Star
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425683-Star
Alexa Fluor® 5465565721-Star
Alexa Fluor® 5555535651-Star
Alexa Fluor® 5685796033-Star
Alexa Fluor® 5945906181-Star
Amplex UltraRed peroxidation product (pH 7.5)5685813-Star
AsRed 25765923-Star
ATTO 5505535763-Star
ATTO 5655635923-Star
BOBO™-35706053-Star
BODIPY TMR-X5445731-Star
BODIPY TR-X (MeOH)5445731-Star
BODIPY TR-X phallacidin5906211-Star
Calcium Crimson5896091-Star
Calcium Orange5896091-Star
CellTracker Red CMTPX5866143-Star
Cy3.5™5785913-Star
Cy3™5545665-Star
Dendra2 (Red)5545691-Star
DiI5515691-Star
DsRed5595833-Star
DsRed-Express5565843-Star
dTomato5565823-Star
DY-5905825993-Star
DyLight 5945936181-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276173-Star
FluoSpheres Red fluorescent microspheres5776073-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846083-Star
HiLyte Fluor™ 5945926161-Star
Hypericin6006031-Star
KFP-Red5755993-Star
LOLO-15685803-Star
LysoTracker Red5735923-Star
Magnesium Orange5505741-Star
mApple5665943-Star
mCherry5876105-Star
Merocyanine 5405595793-Star
MitoTracker™ Orange5515751-Star
MitoTracker™ Red5785983-Star
mKate25886331-Star
mOrange25515651-Star
mRFP5555833-Star
mRFP15847005-Star
mRuby5586053-Star
mStrawberry5745963-Star
mTangerine5685853-Star
Nile red-phospolipid5536371-Star
Orange 5525525741-Star
pHrodo™, succinimidyl ester5605873-Star
Propidium Iodide (PI)3056173-Star
Pro-Q Diamond5565833-Star
Qdot® 585 Nanocrystals3005883-Star
Qdot® 605 Nanocrystals3006023-Star
ReAsH-CCPGCC5926063-Star
Red 5805806033-Star
Resorufin5715843-Star
Rhod-25535773-Star
Rhodamine phalloidin5585751-Star
Rhodamine Red-X5735913-Star
R-phycoerythrin (PE)5655763-Star
SNARF (carboxy) 514 Excitation pH95766381-Star
SNARF-1 488nm (ph 6.0)5485863-Star
SNARF-1 488nm (ph 9.0)5766381-Star
SNARF-1 514nm (ph 6.0)5495873-Star
SNARF-1 514nm (ph 9.0)5766381-Star
SpectrumOrange5545873-Star
Sulforhodamine 101-EtOH5785933-Star
SYTOX Orange5475701-Star
Tetramethylrhodamine dextran5545823-Star
Texas Red dextran5926131-Star
Texas Red DHPE5846083-Star
TRITC (Tetramethylrhodamine)5525783-Star
TurboRFP5535731-Star
X-Rhod-1 Indicator5806013-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556151-Star
Alexa Fluor® 6336316471-Star
Alexa Fluor® 6356336481-Star
Alexa Fluor® 6476536683-Star
Alexa Fluor® 6606636913-Star
APC6516603-Star
APC (Allophycocyanin)6516603-Star
ATTO 6336296583-Star
ATTO 6356356583-Star
ATTO 6476446703-Star
ATTO 647N6456683-Star
ATTO 6556636833-Star
BODIPY 650/665-X6466643-Star
Carboxynaphthofluorescein5996741-Star
Cy5.5™6726901-Star
Cy5™6496665-Star
DDAO6486573-Star
DiD (DiIC18(5)) - Lipophilic Tracer6486703-Star
DY-6306346563-Star
DyLight 6336206491-Star
DyLight 6496516733-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Dark Red fluorescent microspheres6566833-Star
HCS CellMask Red cytoplasmic6226441-Star
HCS LipidTOX Deep Red neutral lipid stain6356521-Star
HiLyte Fluor™ 6476496733-Star
LIVE-DEAD Fixable Far Red Dead Cell Stain6536683-Star
MitoTracker™ Deep Red6416623-Star
Nile blue-EtOH6316603-Star
Qdot® 705 Nanocrystals3007021-Star
Red 6506496653-Star
SpectrumFRed (Far Red)6506733-Star
SYTO 596226441-Star
SYTO 606506813-Star
SYTO 616186501-Star
SYTOX Red-DNA6406583-Star
TO-PRO-36426573-Star
TOTO-36426613-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Flatness of Dichroic BeamsplittersDescribes the impact of non-flat dichroic beamsplitters on microscope image fidelity
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Optical Filters for Laser-based Fluorescence MicroscopesDiscusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
Journal Article
TopicDescription
Bringing Super-resolution to Fluorescence MicroscopyPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioPhotonics, May/June 2010
Advances in fluorescence microscopy techniques allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Fluorescence Imaging: Optical filters optimize laser-based fluorescence imaging systemsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., Laser Focus World, January 2010
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
Perfecting TIRF OpticsPrashant Prabhat, PhD, and Turan Erdogan, PhD, Semrock Inc., BioOptics World, Jan/Feb 2009
Discusses additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection
White Paper
TopicDescription
Optical Filters for Laser-based Fluorescence MicroscopesAnalysis of additional considerations that laser excitation imposes on traditional fluorescence microscopy filter selection with a focus on multiple-laser systems.
Super-resolution MicroscopyDiscusses advancements in fluorescence microscopy techniques that allow users to break the defraction limit and visualize resolution similar to that of an electron microscope
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
TIRF (Total Internal Reflection Fluorescence)An overview of TIRF microscopy, including solutions from Semrock and additional learning resources.
Laser Scanning/Spinning Disk Confocal MicroscopyAn overview of confocal microscopy techniques and applications, including solutions from Semrock and additional learning resources.
Laser-based InstrumentationAn review of common of laser-based instrumentation, including solutions from Semrock and additional learning resources.



LF405/488/543/635-4X-A 405/488/543/635 R405/488/543/635

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