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BrightLine® single-band filter set, optimized for Qdot 605 Nanocrystals and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: QD605-C-000

Fluorophore Displayed:
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Semrock Filter Set: QD605-C

BrightLine® single-band filter set, optimized for Qdot 605 Nanocrystals and other like fluorophores

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 605 emission with minimal crosstalk from neighboring Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details QD605-C-000 (Standard size set, fits most microscopes2)$785In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-435/40-25$355
Single Band Emitter25 mm x 3.5 mmFF01-605/15-25$355
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF510-Di02-25x36$335
Click to expand/collapse details QD605-C-000-ZERO (Standard size -ZERO pixel set)$884In StockAdd To Cart
Click to expand/collapse details QD605-C-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details QD605-C-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details QD605-C-NQF (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details QD605-C-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details QD605-C-NTE (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details QD605-C-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details QD605-C-OMF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details QD605-C-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details QD605-C-OFF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details QD605-C-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details QD605-C-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details QD605-C-ZHE (set mounted in cube)$1,060In StockAdd To Cart
Click to expand/collapse details QD605-C-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details QD605-C-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details QD605-C-AMF-ZERO (-ZERO pixel set mounted in cube)$1,349In StockAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 4304315411-Star
Cascade Yellow™3995491-Star
FM 1-434735791-Star
Lucifer yellow4285441-Star
Pacific Orange4005511-Star
Qdot® 585 Nanocrystals3005881-Star
Qdot® 605 Nanocrystals3006025-Star
SYPRO Ruby protein gel stain4696311-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Fluorescence Imaging with Quantum Dot NanocrystalsDescribes how the Qdot nanocrystal structure is unique and the Semrock filter sets available for use with Qdots.
Journal Article
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.

QD605-C QDot605 QDOT Nanocrystals FF02-435/40 FF01-605/15 FF510-Di02 FF510 435/40 605/15 510 Filter Filters Set Sets QD605-C-000 QD605-C-000-ZERO QD605-C-L01-ZERO QD605-C-U02 QD605-C-NQF QD605-C-NQF-ZERO QD605-C-NTE QD605-C-NTE-ZERO QD605-C-OMF QD605-C-OMF-ZERO QD605-C-OFF QD605-C-OFF-ZERO QD605-C-OFX QD605-C-ZHE QD605-C-ZHE-ZERO QD605-C-LDMK-ZERO QD605-C-AMF-ZERO

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