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BrightLine^® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: QD525-C-000

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Filters and Pricing
Specifications
Fluorophore
Technical Info

BrightLine^® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 525 emission with minimal crosstalk from longer-wavelength Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

Individual filters and filter sets (no cube):

These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.

Part Number

Price¹

Stock Status

Quantity

QD525-C-000 (Standard size set, fits most microscopes²)

$785

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335

QD525-C-000-ZERO (Standard size -ZERO pixel set)

$884

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
-ZERO set certification		-ZERO	$99

QD525-C-L01-ZERO (Leica 'Large' -ZERO pixel set)

$884

Ships in 2 days

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	21.8 mm x 2.0 mm	FF02-435/40-21.8-D	$355
Single Band Emitter	23.3 mm x 2.0 mm	FF01-525/15-23.3-D	$355
Single Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF510-Di02-22x29	$335
-ZERO set certification		-ZERO	$99

Filter Role	Filter Size	Part Number	Price¹
-ZERO set certification		-ZERO	$99

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	32 mm x 3.5 mm	FF01-525/15-32	$582
Single Band Dichroic	32 x 44 x 1.1 mm (corners cut for OXL cube)	FF510-Di02-32x44-XL	$1

QD525-C-U02 (Olympus large-size (FX) set for U-M710I)

$1,288

Ships in 2 days

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	32 mm x 5.0 mm	FF02-435/40-32	$582
Single Band Emitter	32 mm x 3.5 mm	FF01-525/15-32	$582
Single Band Dichroic	32 x 44 x 1.1 mm (corners cut for OFX cube)	FF510-Di02-32x44-FX	$526

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:

These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.

Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series

QD525-C-NQF (set mounted in cube)

$1,180

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
Quadfluor cube		NQF	$395

QD525-C-NQF-ZERO (-ZERO pixel set mounted in cube)

$1,279

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
Quadfluor cube		NQF	$395
-ZERO set certification		-ZERO	$99

Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator

QD525-C-NTE (set mounted in cube)

$1,180

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
TE 2000 cube		NTE	$395

QD525-C-NTE-ZERO (-ZERO pixel set mounted in cube)

$1,279

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
TE 2000 cube		NTE	$395
-ZERO set certification		-ZERO	$99

Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81

QD525-C-OMF (set mounted in cube)

$1,220

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
U-MF2 cube		OMF	$435

QD525-C-OMF-ZERO (-ZERO pixel set mounted in cube)

$1,319

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
U-MF2 cube		OMF	$435
-ZERO set certification		-ZERO	$99

Olympus U-FF for microscopes: BX53, BX63

QD525-C-OFF (set mounted in cube)

$1,220

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
U-FF cube		OFF	$435

QD525-C-OFF-ZERO (-ZERO pixel set mounted in cube)

$1,319

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
U-FF cube		OFF	$435
-ZERO set certification		-ZERO	$99

Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes

QD525-C-OFX (set mounted in cube)

$1,883

Ships in 2 days

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	32 mm x 5.0 mm	FF02-435/40-32	$582
Single Band Emitter	32 mm x 3.5 mm	FF01-525/15-32	$582
Single Band Dichroic	32 x 44 x 1.1 mm (corners cut for OFX cube)	FF510-Di02-32x44-FX	$526
U-M710I cube		OFX	$595

Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1

QD525-C-ZHE (set mounted in cube)

$1,060

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
FL Cube EC P+C cube		ZHE	$275

QD525-C-ZHE-ZERO (-ZERO pixel set mounted in cube)

$1,159

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
FL Cube EC P+C cube		ZHE	$275
-ZERO set certification		-ZERO	$99

Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B

QD525-C-LDMK-ZERO (-ZERO pixel set mounted in cube)

$1,234

Ships in 2 days

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	21.8 mm x 2.0 mm	FF02-435/40-21.8-D	$355
Single Band Emitter	23.3 mm x 2.0 mm	FF01-525/15-23.3-D	$355
Single Band Dichroic	22.0 mm x 29.0 mm x 1.1 mm	FF510-Di02-22x29	$335
DM-K cube		LDMK	$350
-ZERO set certification		-ZERO	$99

Aperio AMF for microscopes: ScanScope FL

QD525-C-AMF-ZERO (-ZERO pixel set mounted in cube)

$1,349

In Stock

Filter Role	Filter Size	Part Number	Price¹
Single Band Exciter	25 mm x 5.0 mm	FF02-435/40-25	$355
Single Band Emitter	25 mm x 3.5 mm	FF01-525/15-25	$355
Single Band Dichroic	25.2 mm x 35.6 mm x 1.1 mm	FF510-Di02-25x36	$335
AMF cube		AMF	$465
-ZERO set certification		-ZERO	$99

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.

Optical Specifications

Specification	Value
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications

Specification	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm²) for over 500 hours.

Physical Filter Specifications

Specification	Value
Scratch-Dig	60-40
Exciter/Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation	"Reflective coating side" should face toward light source and sample.

Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength (nm)	Peak Emission Wavelength (nm)	Compatibility	Display on Plot above
Acridine Yellow	470	550
Alexa Fluor^® 430	431	541
AmCyan1	458	489
ATTO 425	436	484
ATTO 465	453	507
BCECF (pH 5.5)	481	518
BD Horizon V500	410	500
BOBO™-1	461	484
Cascade Yellow™	399	549
CellTracker Violet BMQC+GSH	406	526
Cerulean	434	473
CFP (ECFP)	433	475
DEAC	432	472
ECFP	435	475
evoglow-Bs1	449	496
GFP (EGFP)	489	511
Lucifer yellow	428	544
LysoSensor Green	448	502
mBBr+GSH	394	490
mHoneyDew	478	562
NBD-X (MeOH)	467	538
Pacific Orange	400	551
Qdot^® 525 Nanocrystals	300	525
sgGFP™ (super glow GFP)	472	506
SpectrumAqua	434	481
SYTO 45	451	485
TagCFP	458	480
ThiolTracker Violet+GSH	404	526
wtGFP (wild type GFP, non-UV excitation)	474	509

Tech Note

Topic	Description
Cleaning Optical Filters	Instructions for cleaning hard coated optical filters
Cube Assembly Instructions	PDF and video instruction for installing filters into popular filter cubes
Filter Reliability	Compares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence Filters	Shows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a Microscope	A guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?	Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Fluorescence Imaging with Quantum Dot Nanocrystals	Describes how the Qdot nanocrystal structure is unique and the Semrock filter sets available for use with Qdots.

Journal Article

Topic	Description
Achieving the Best Alignment for Fluorescent Images	Turan Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005 Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image

White Paper

Topic	Description
Spectral Modeling in Fluorescence Microscopy	Discusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection

Application

Topic	Description
High Performance Microscopy	An overview of high performance microscopy, including solutions from Semrock and additional learning resources.

QD525-C QDot525 QDOT Nanocrystals FF01-525/15 FF02-435/40 FF510-Di02 FF510 525/15 435/40 510 Filter Filters Set Sets QD525-C-000 QD525-C-000-ZERO QD525-C-L01-ZERO QD525-C-U02 QD525-C-NQF QD525-C-NQF-ZERO QD525-C-NTE QD525-C-NTE-ZERO QD525-C-OMF QD525-C-OMF-ZERO QD525-C-OFF QD525-C-OFF-ZERO QD525-C-OFX QD525-C-ZHE QD525-C-ZHE-ZERO QD525-C-LDMK-ZERO QD525-C-AMF-ZERO

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Semrock manufactures hundreds of standard optical filters for immediate shipping: bandpass filters, dichroic beamsplitters, edge and notch filters, Raman spectroscopy filters, mirrors and fluorescence filters sets. For Custom optical filters, engineering design or system design support, please contact us to discuss your specific needs.

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BrightLine® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

Part Number: QD525-C-000

BrightLine® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

BrightLine^® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

BrightLine^® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores