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BrightLine® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: QD525-C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: QD525-C

BrightLine® single-band filter set, optimized for Qdot 525 Nanocrystals and other like fluorophores

This single-band filter set is perfectly optimized for brilliant, dense multi-color detection with quantum dot nanocrystals. The highly transmitting, deep-blue exciter achieves maximum quantum dot excitation efficiency while virtually eliminating any DAPI or Hoechst excitation. The emitter has high transmission, steep edges, and a narrow bandwidth to perfectly match the Qdot 525 emission with minimal crosstalk from longer-wavelength Qdots. With its exceptionally wide and flat transmission and reflection bands, the dichroic not only accommodates all Qdot nanocrystals but does so with a wide range of possible UV and blue excitation wavelengths. And with the "no burn-out" performance shared by all BrightLine filters, this set is the ideal tool for applications that depend on the superb photostability and archivability of quantum dots.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details QD525-C-000 (Standard size set, fits most microscopes2)$825In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-435/40-25$355
Single Band Emitter25 mm x 3.5 mmFF01-525/15-25$355
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF510-Di02-25x36$335
Click to expand/collapse details QD525-C-000-ZERO (Standard size -ZERO pixel set)$924In StockAdd To Cart
Click to expand/collapse details QD525-C-L01-ZERO (Leica 'Large' -ZERO pixel set)$924Ships in 2 daysAdd To Cart
Click to expand/collapse details QD525-C-U02 (Olympus large-size (FX) set for U-M710I)$1,370Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details QD525-C-NQF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details QD525-C-NQF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details QD525-C-NTE (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details QD525-C-NTE-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details QD525-C-OMF (set mounted in cube)$1,260In StockAdd To Cart
Click to expand/collapse details QD525-C-OMF-ZERO (-ZERO pixel set mounted in cube)$1,359In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details QD525-C-OFF (set mounted in cube)$1,260In StockAdd To Cart
Click to expand/collapse details QD525-C-OFF-ZERO (-ZERO pixel set mounted in cube)$1,359In StockAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details QD525-C-OFX (set mounted in cube)$2,065Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details QD525-C-ZHE (set mounted in cube)$1,100In StockAdd To Cart
Click to expand/collapse details QD525-C-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,199In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details QD525-C-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,274Ships in 2 daysAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details QD525-C-AMF-ZERO (-ZERO pixel set mounted in cube)$1,389In StockAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Acridine Yellow4705501-Star
Alexa Fluor® 4304315413-Star
ATTO 4254364841-Star
ATTO 4654535073-Star
BCECF (pH 5.5)4815181-Star
BD Horizon V5004105001-Star
Cascade Yellow™3995491-Star
CellTracker Violet BMQC+GSH4065261-Star
CFP (ECFP)4334751-Star
GFP (EGFP)4895111-Star
Lucifer yellow4285443-Star
LysoSensor Green4485023-Star
NBD-X (MeOH)4675381-Star
Pacific Orange4005511-Star
Qdot® 525 Nanocrystals3005255-Star
sgGFP™ (super glow GFP)4725061-Star
SYTO 454514851-Star
ThiolTracker Violet+GSH4045261-Star
wtGFP (wild type GFP, non-UV excitation)4745091-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Fluorescence Imaging with Quantum Dot NanocrystalsDescribes how the Qdot nanocrystal structure is unique and the Semrock filter sets available for use with Qdots.
Journal Article
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.

QD525-C QDot525 QDOT Nanocrystals FF01-525/15 FF02-435/40 FF510-Di02 FF510 525/15 435/40 510 Filter Filters Set Sets QD525-C-000 QD525-C-000-ZERO QD525-C-L01-ZERO QD525-C-U02 QD525-C-NQF QD525-C-NQF-ZERO QD525-C-NTE QD525-C-NTE-ZERO QD525-C-OMF QD525-C-OMF-ZERO QD525-C-OFF QD525-C-OFF-ZERO QD525-C-OFX QD525-C-ZHE QD525-C-ZHE-ZERO QD525-C-LDMK-ZERO QD525-C-AMF-ZERO

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