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BrightLine® Sedat filter set, optimized for Green Fluorescent Protein & DsRed and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: GFP/DsRed-2X2M-C-000

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Semrock Filter Set: GFP/DsRed-2X2M-C

BrightLine® Sedat filter set, optimized for Green Fluorescent Protein & DsRed and other like fluorophores

This 5-filter "Sedat" set is ideal for high-speed sequential imaging GFP and DsRed with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The set is also ideal for samples dual-labeled with FITC and TRITC, or GFP and Cy3, among o

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details GFP/DsRed-2X2M-C-000 (Standard size set, fits most microscopes2)$1,435Ships in 2 daysAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-470/22-25$305
Single Band Exciter25 mm x 5.0 mmFF01-556/20-25$355
Single Band Emitter25 mm x 3.5 mmFF01-514/30-25$355
Single Band Emitter25 mm x 5.0 mmFF02-617/73-25$355
Dual Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF493/574-Di01-25x36$365
Click to expand/collapse details GFP/DsRed-2X2M-C-L01 (Leica 'Large' set)$1,435Ships in 2 daysAdd To Cart
Click to expand/collapse details GFP/DsRed-2X2M-C-S01 (Sutter threaded set)$1,515Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details GFP/DsRed-2X2M-C-NQF (set mounted in cube)$1,830Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details GFP/DsRed-2X2M-C-NTE (set mounted in cube)$1,830Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details GFP/DsRed-2X2M-C-OMF (set mounted in cube)$1,870Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details GFP/DsRed-2X2M-C-OFF (set mounted in cube)$1,870Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details GFP/DsRed-2X2M-C-ZHE (set mounted in cube)$1,710Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details GFP/DsRed-2X2M-C-LDMK (set mounted in cube)$1,785Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details GFP/DsRed-2X2M-C-LDMP (set mounted in cube)$1,870Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-FAM (5-carboxyfluorescein)4925181-Star
Acridine Orange (+DNA)5025261-Star
Acridine Yellow4705501-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995203-Star
AmCyan14584893-Star
ATTO 4654535071-Star
ATTO 4885005251-Star
ATTO 4954955271-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025281-Star
BOBO™-14614841-Star
BODIPY FL5055121-Star
Calcein4945141-Star
CFP (ECFP)4334751-Star
CFP24905101-Star
Cy2™4925073-Star
CyQUANT GR-DNA5025231-Star
DAF-FM-NO4955191-Star
Dendra2 (Green)4915071-Star
DiO4755001-Star
Dronpa5025161-Star
ECFP4354751-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915111-Star
evoglow-Bs14494961-Star
FITC (Fluorescein)4955195-Star
Fluo-44945161-Star
Fluorescein dextran5015241-Star
Fluorescein-pH 8.04895171-Star
Fluoro-Emerald4945181-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
GFP (EGFP)4895115-Star
Green 4964965201-Star
Green 5005015241-Star
HiLyte Fluor™ 4884975261-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985251-Star
LysoSensor Green4485021-Star
LysoTracker Green5015091-Star
LysoTracker Yellow HCK-1234885651-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905121-Star
mTFP14624921-Star
mWasabi4935091-Star
NBD-X (MeOH)4675381-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
Oregon Green™ 4884985261-Star
PicoGreen®5025221-Star
PKH674885003-Star
Qdot® 525 Nanocrystals3005251-Star
Rhodamine 1104975191-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
rsGFP (red shifted GFP, S65T)4985163-Star
sgGFP™ (super glow GFP)4725063-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYTO 134485061-Star
SYTO 164895201-Star
SYTO 454514851-Star
SYTO 94835003-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
TagCFP4584801-Star
TurboGFP4825033-Star
wtGFP (wild type GFP, non-UV excitation)4745093-Star
YO-PRO-14915061-Star
YOYO-14915081-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825953-Star
5-ROX (carboxy-X-rhodamine)5786041-Star
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425683-Star
7-AAD (7-aminoactinomycin D)5486483-Star
Alexa Fluor® 5465565721-Star
Alexa Fluor® 5555535651-Star
Alexa Fluor® 5685796035-Star
Alexa Fluor® 5945906183-Star
Amplex UltraRed peroxidation product (pH 7.5)5685813-Star
AsRed 25765921-Star
ATTO 5505535763-Star
ATTO 5655635923-Star
ATTO 5905946241-Star
BOBO™-35706053-Star
BODIPY TMR-X5445731-Star
BODIPY TR-X (MeOH)5445731-Star
BODIPY TR-X phallacidin5906211-Star
Calcium Crimson5896091-Star
Calcium Orange5896093-Star
CellTrace BODIPY TR methyl ester5986251-Star
CellTracker Red CMTPX5866141-Star
Cy3.5™5785911-Star
Cy3™5545663-Star
DDAO6486571-Star
Dendra2 (Red)5545691-Star
DiI5515691-Star
DsRed5595835-Star
DsRed-Express5565843-Star
dTomato5565823-Star
DY-5905825991-Star
DyLight 5945936181-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276171-Star
FluoSpheres Red fluorescent microspheres5776071-Star
HcRed15886181-Star
HCS LipidTOX Red neutral lipid stain5826161-Star
HCS LipidTOX Red phospholipidosis5846081-Star
HiLyte Fluor™ 5945926161-Star
KFP-Red5755991-Star
LOLO-15685803-Star
LysoTracker Red5735921-Star
Magnesium Orange5505743-Star
mApple5665943-Star
mCherry5876101-Star
Merocyanine 5405595793-Star
MitoTracker™ Orange5515753-Star
MitoTracker™ Red5785981-Star
mKate25886331-Star
mOrange5465621-Star
mOrange25515651-Star
mPlum5896491-Star
mRFP5555833-Star
mRFP15847001-Star
mRuby5586053-Star
mStrawberry5745961-Star
mTangerine5685853-Star
Nile red-phospolipid5536373-Star
Nile red-triglyceride5105831-Star
OFP5475671-Star
Orange 5525525743-Star
pHrodo™, succinimidyl ester5605873-Star
POPO-35335741-Star
Propidium Iodide (PI)3056173-Star
Pro-Q Diamond5565833-Star
Qdot® 585 Nanocrystals3005881-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Qdot® 655 Nanocrystals3006541-Star
ReAsH-CCPGCC5926061-Star
Red 5805806031-Star
Resorufin5715843-Star
Rhod-25535773-Star
Rhodamine phalloidin5585753-Star
Rhodamine Red-X5735911-Star
R-phycoerythrin (PE)5655763-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 6.0)5485863-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 6.0)5495873-Star
SNARF-1 514nm (ph 9.0)5766383-Star
SpectrumOrange5545873-Star
Sulforhodamine 101-EtOH5785931-Star
SYTOX Orange5475701-Star
Tetramethylrhodamine dextran5545823-Star
Texas Red dextran5926131-Star
Texas Red DHPE5846081-Star
Texas Red-X®5956133-Star
TRITC (Tetramethylrhodamine)5525785-Star
TurboFP635(Katushka)5916381-Star
TurboRFP5535731-Star
X-Rhod-1 Indicator5806011-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



GFP/DsRed-2X2M-C GFP/DsRed GFP DsRed Sedat Dual-Band FF01-514/30 FF01-470/22 FF493/574-Di01 FF02-617/73 514/30 470/22 FF493/574 493/574 617/73 Filter Filters Set Sets Dual 2X2M GFP/DsRed-2X2M-C-000 GFP/DsRed-2X2M-C-L01 GFP/DsRed-2X2M-C-S01 GFP/DsRed-2X2M-C-NQF GFP/DsRed-2X2M-C-NTE GFP/DsRed-2X2M-C-OMF GFP/DsRed-2X2M-C-OFF GFP/DsRed-2X2M-C-ZHE GFP/DsRed-2X2M-C-LDMP GFP/DsRed-2X2M-C-LDMK

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