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BrightLine® single-band filter set, optimized for Cy5 and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: Cy5-4040C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: Cy5-4040C

BrightLine® single-band filter set, optimized for Cy5 and other like fluorophores

This filter set is ideal for use with Cy5™ and other deep red fluorophores. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details Cy5-4040C-000 (Standard size set, fits most microscopes2)$785In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-628/40-25$305
Single Band Emitter25 mm x 3.5 mmFF01-692/40-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF660-Di02-25x36$255
Click to expand/collapse details Cy5-4040C-000-ZERO (Standard size -ZERO pixel set)$884In StockAdd To Cart
Click to expand/collapse details Cy5-4040C-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details Cy5-4040C-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details Cy5-4040C-NQF (set mounted in cube)$1,180Ships in 2 daysAdd To Cart
Click to expand/collapse details Cy5-4040C-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details Cy5-4040C-NTE (set mounted in cube)$1,180Ships in 2 daysAdd To Cart
Click to expand/collapse details Cy5-4040C-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details Cy5-4040C-OMF (set mounted in cube)$1,220Ships in 2 daysAdd To Cart
Click to expand/collapse details Cy5-4040C-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details Cy5-4040C-OFF (set mounted in cube)$1,220Ships in 2 daysAdd To Cart
Click to expand/collapse details Cy5-4040C-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details Cy5-4040C-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details Cy5-4040C-ZHE (set mounted in cube)$1,060Ships in 2 daysAdd To Cart
Click to expand/collapse details Cy5-4040C-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details Cy5-4040C-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details Cy5-4040C-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details Cy5-4040C-AMF-ZERO (-ZERO pixel set mounted in cube)$1,349Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556151-Star
Alexa Fluor® 6476536685-Star
Alexa Fluor® 6606636915-Star
Alexa Fluor® 6806797021-Star
APC6516601-Star
APC (Allophycocyanin)6516601-Star
ATTO 6206186421-Star
ATTO 6336296581-Star
ATTO 6356356581-Star
ATTO 6476446703-Star
ATTO 647N6456683-Star
ATTO 6556636831-Star
ATTO 6806807001-Star
BODIPY 650/665-X6466645-Star
Carboxynaphthofluorescein5996741-Star
Cy5.5™6726901-Star
Cy5™6496665-Star
DDAO6486571-Star
DiD (DiIC18(5)) - Lipophilic Tracer6486705-Star
DY-6306346561-Star
DyLight 6496516733-Star
DyLight 6806767051-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Dark Red fluorescent microspheres6566831-Star
HCS CellMask Red cytoplasmic6226441-Star
HiLyte Fluor™ 6476496733-Star
LDS 7515607111-Star
LIVE-DEAD Fixable Far Red Dead Cell Stain6536683-Star
MitoTracker™ Deep Red6416621-Star
Nile blue-EtOH6316601-Star
Qdot® 705 Nanocrystals3007021-Star
Red 6506496653-Star
SpectrumFRed (Far Red)6506735-Star
SYTO 596226441-Star
SYTO 606506815-Star
SYTO 616186501-Star
SYTO Red Fluorescent Nucleic Acid Stains 606556753-Star
SYTO Red Fluorescent Nucleic Acid Stains 626556753-Star
SYTO Red Fluorescent Nucleic Acid Stains 636556753-Star
SYTOX Red-DNA6406581-Star
TO-PRO-36426575-Star
TOTO-36426615-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
FISH (Fluorescence in Situ Hybridization)An overview of FISH, including solutions from Semrock and additional learning resources.



Cy5-4040C Cy5 692/40 660 628/40 Single-Band FF01-692/40 FF660-Di02 FF02-628/40 FF660 Filter Filters Set Sets Cy5-4040C-000 Cy5-4040C-000-ZERO Cy5-4040C-L01-ZERO Cy5-4040C-U02 Cy5-4040C-NQF Cy5-4040C-NQF-ZERO Cy5-4040C-NTE Cy5-4040C-NTE-ZERO Cy5-4040C-OMF Cy5-4040C-OMF-ZERO Cy5-4040C-OFF Cy5-4040C-OFF-ZERO Cy5-4040C-OFX Cy5-4040C-ZHE Cy5-4040C-ZHE-ZERO Cy5-4040C-LDMP-ZERO Cy5-4040C-LDMK-ZERO Cy5-4040C-AMF-ZERO

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