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BrightLine® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: GFP-3035D-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: GFP-3035D

BrightLine® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

This filter set is optimized to be simultaneously a bright and high signal-to-noise ratio (contrast) for measuring GFP and its variants, allowing for no need to exchange filter sets for different GFP experiments.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details GFP-3035D-000 (Standard size set, fits most microscopes2)$785In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-472/30-25$305
Single Band Emitter25 mm x 3.5 mmFF01-520/35-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF495-Di03-25x36$255
Click to expand/collapse details GFP-3035D-000-ZERO (Standard size -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details GFP-3035D-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details GFP-3035D-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details GFP-3035D-NQF (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details GFP-3035D-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details GFP-3035D-NTE (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details GFP-3035D-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details GFP-3035D-OMF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details GFP-3035D-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details GFP-3035D-OFF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details GFP-3035D-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details GFP-3035D-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details GFP-3035D-ZHE (set mounted in cube)$1,060In StockAdd To Cart
Click to expand/collapse details GFP-3035D-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details GFP-3035D-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details GFP-3035D-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details GFP-3035D-AMF-ZERO (-ZERO pixel set mounted in cube)$1,349Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-FAM (5-carboxyfluorescein)4925183-Star
Acridine Orange (+DNA)5025261-Star
Acridine Yellow4705501-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995201-Star
Alexa Fluor® 5005035251-Star
AmCyan14584893-Star
ATTO 4654535073-Star
ATTO 4885005251-Star
ATTO 4954955271-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025281-Star
BOBO™-14614841-Star
BODIPY FL5055121-Star
Calcein4945141-Star
CFP (ECFP)4334751-Star
CFP24905103-Star
Cy2™4925075-Star
CyQUANT GR-DNA5025231-Star
DAF-FM-NO4955191-Star
Dendra2 (Green)4915073-Star
DiO4755005-Star
Dronpa5025161-Star
ECFP4354751-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915113-Star
evoglow-Bs14494961-Star
FITC (Fluorescein)4955191-Star
Fluo-44945161-Star
Fluorescein dextran5015241-Star
Fluorescein-pH 8.04895171-Star
Fluoro-Emerald4945181-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
GFP (EGFP)4895115-Star
Green 4964965201-Star
Green 5005015241-Star
HCS LipidTOX Green phospholipidosis5045361-Star
HiLyte Fluor™ 4884975261-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985251-Star
LysoSensor Green4485021-Star
LysoTracker Green5015091-Star
LysoTracker Yellow HCK-1234885651-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905123-Star
mTFP14624921-Star
mWasabi4935091-Star
NBD-X (MeOH)4675383-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
Oregon Green™ 4884985261-Star
PicoGreen®5025221-Star
PKH674885003-Star
Qdot® 525 Nanocrystals3005251-Star
Rhodamine 1104975191-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
sgGFP™ (super glow GFP)4725065-Star
Sodium Green5075321-Star
SpectrumGreen4975381-Star
SYBR Gold nucleic acid gel stain-DNA4965391-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895203-Star
SYTO 454514851-Star
SYTO 94835003-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
TagCFP4584801-Star
TOTO-15145311-Star
TurboGFP4825033-Star
Vybrant DyeCycle Green5065341-Star
wtGFP (wild type GFP, non-UV excitation)4745095-Star
YO-PRO-14915065-Star
YOYO-14915085-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



GFP-3035D GFP 472/30 520/35 495 Single-Band FF01-520/35 FF02-472/30 FF495-Di03 FF495 Filter Filters Set Sets GFP-3035D-000 GFP-3035D-000-ZERO GFP-3035D-L01-ZERO GFP-3035D-U02 GFP-3035D-NQF GFP-3035D-NQF-ZERO GFP-3035D-NTE GFP-3035D-NTE-ZERO GFP-3035D-OMF GFP-3035D-OMF-ZERO GFP-3035D-OFF GFP-3035D-OFF-ZERO GFP-3035D-OFX GFP-3035D-ZHE GFP-3035D-ZHE-ZERO GFP-3035D-LDMP-ZERO GFP-3035D-LDMK-ZERO GFP-3035D-AMF-ZERO

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