Welcome, Guest: Login or Register My Cart (0)

BrightLine® Pinkel filter set, optimized for FITC & Texas Red and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: FITC/TXRED-2X-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: FITC/TXRED-2X-B

BrightLine® Pinkel filter set, optimized for FITC & Texas Red and other like fluorophores

This 4-filter "Pinkel" set is perfectly optimized for imaging FITC and Texas Red with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is also very popular for imaging GFP and mCherry. It is comprised of two single-band exciters along with a dual-band beamsplitter and a dual-band emitter, and thus works best with an exciter filter wheel or slider.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details FITC/TXRED-2X-B-000 (Standard size set, fits most microscopes2)$1,255In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-485/20-25$305
Single Band Exciter25 mm x 5.0 mmFF01-586/20-25x5$305
Dual Band Emitter25 mm x 3.5 mmFF01-524/628-25$425
Dual Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF505/606-Di01-25x36$365
Click to expand/collapse details FITC/TXRED-2X-B-L01 (Leica 'Large' set)$1,255Ships in 2 daysAdd To Cart
Click to expand/collapse details FITC/TXRED-2X-B-S01 (Sutter threaded set)$1,295Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details FITC/TXRED-2X-B-NQF (set mounted in cube)$1,650In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details FITC/TXRED-2X-B-NTE (set mounted in cube)$1,650In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details FITC/TXRED-2X-B-OMF (set mounted in cube)$1,690In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details FITC/TXRED-2X-B-OFF (set mounted in cube)$1,690In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details FITC/TXRED-2X-B-ZHE (set mounted in cube)$1,530In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details FITC/TXRED-2X-B-LDMK (set mounted in cube)$1,605Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details FITC/TXRED-2X-B-LDMP (set mounted in cube)$1,690In StockAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005251-Star
5-FAM (5-carboxyfluorescein)4925183-Star
Acridine Orange (+DNA)5025261-Star
Alexa Fluor® 4884995205-Star
Alexa Fluor® 5005035251-Star
AmCyan14584891-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025283-Star
BODIPY FL5055121-Star
BODIPY FL-MeOH5025111-Star
Calcein4945143-Star
Calcium Green-15075291-Star
CFP24905103-Star
Cy2™4925071-Star
CyQUANT GR-DNA5025231-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915073-Star
DiO4755003-Star
Dronpa5025161-Star
DY-505-Phalloidin5035301-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915113-Star
FITC (Fluorescein)4955195-Star
FlAsH-CCPFCC5115301-Star
Fluo-35065271-Star
Fluo-44945163-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895173-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
GFP (EGFP)4895115-Star
Green 4964965203-Star
Green 5005015243-Star
HCS LipidTOX Green neutral lipid stain4985073-Star
HCS LipidTOX Green phospholipidosis5045361-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoSensor Green4485021-Star
LysoTracker Green5015093-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075311-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905123-Star
mWasabi4935093-Star
NBD-X (MeOH)4675381-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135321-Star
PicoGreen®5025221-Star
PKH674885001-Star
Qdot® 525 Nanocrystals3005251-Star
ratiometric pHluorin pH55005093-Star
Rhodamine 1104975193-Star
Rhodamine 1235075291-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
sgGFP™ (super glow GFP)4725063-Star
Sodium Green5075321-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895203-Star
SYTO 94835001-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboGFP4825033-Star
Vybrant DyeCycle Green5065341-Star
wtGFP (wild type GFP, non-UV excitation)4745093-Star
YFP (yellow GFP)5135301-Star
YO-PRO-14915063-Star
YOYO-14915083-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825951-Star
5-ROX (carboxy-X-rhodamine)5786041-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5685796035-Star
Alexa Fluor® 5945906185-Star
AsRed 25765921-Star
ATTO 5905946243-Star
ATTO 5946016243-Star
ATTO 6106146341-Star
BOBO™-35706051-Star
BODIPY 630/650-X6256411-Star
BODIPY TR-X phallacidin5906213-Star
Calcium Crimson5896093-Star
CellTrace BODIPY TR methyl ester5986253-Star
CellTracker Red CMTPX5866143-Star
Cy3.5™5785911-Star
DY-5905825991-Star
DyLight 5945936183-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Red fluorescent microspheres5776073-Star
HcRed15886183-Star
HCS CellMask Red cytoplasmic6226441-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846083-Star
HiLyte Fluor™ 5945926163-Star
Hypericin6006031-Star
KFP-Red5755991-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956133-Star
LysoTracker Red5735921-Star
mApple5665941-Star
mCherry5876105-Star
MitoTracker™ Red5785981-Star
mKate25886333-Star
mPlum5896491-Star
mRFP15847003-Star
mStrawberry5745961-Star
Nile red-phospolipid5536371-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
ReAsH-CCPGCC5926063-Star
Red 5805806031-Star
Rhodamine Red-X5735911-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 9.0)5766383-Star
SpectrumRed5876153-Star
Sulforhodamine 101-EtOH5785931-Star
SYTO 176196381-Star
SYTO 596226441-Star
SYTO 616186501-Star
Texas Red dextran5926135-Star
Texas Red DHPE5846085-Star
Texas Red®5926145-Star
TurboFP635(Katushka)5916383-Star
X-Rhod-1 Indicator5806011-Star
YO-PRO-36136291-Star
YOYO-36116311-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



FITC/TXRED-2X-B FITC/TxRed FITC/TexasRed FI/TX FITC TexasRed TxRed Pinkel Dual-Band FF01-524/628 FF02-485/20 FF01-586/20 FF505/606-Di01 FF505/606 524/628 485/20 586/20 505/606 Filter Filters Set Sets Dual 2X FITC/TXRED-2X-B-000 FITC/TXRED-2X-B-L01 FITC/TXRED-2X-B-S01 FITC/TXRED-2X-B-NQF FITC/TXRED-2X-B-NTE FITC/TXRED-2X-B-OMF FITC/TXRED-2X-B-OFF FITC/TXRED-2X-B-ZHE FITC/TXRED-2X-B-LDMP FITC/TXRED-2X-B-LDMK

The item has been added to your shopping cart.

The item has been added to your shopping cart, however, your IP address appears to be outside the US/ Canada, so online/ direct orders from Semrock are not allowed. Please see the International Distributor page to find your non-US distributor.

We apologize for any inconvenience.

The shopping cart is offline. Please call toll-free 1-866-SEMROCK (866-736-7625) or email us to place your order.