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BrightLine® Pinkel filter set, optimized for Cy3 & Cy5 and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: Cy3/Cy5-2X-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: Cy3/Cy5-2X-B

BrightLine® Pinkel filter set, optimized for Cy3 & Cy5 and other like fluorophores

This 4-filter "Pinkel" set is perfectly optimized for imaging Cy3 and Cy5 with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. It is comprised of two single-band exciters along with a dual-band beamsplitter and a dual-band emitter, and thus works best with an exciter filter wheel or slider.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details Cy3/Cy5-2X-B-000 (Standard size set, fits most microscopes2)$1,255In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF02-534/30-25$305
Single Band Exciter25 mm x 5.0 mmFF02-628/40-25$305
Dual Band Emitter25 mm x 3.5 mmFF01-577/690-25$425
Dual Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF560/659-Di01-25x36$365
Click to expand/collapse details Cy3/Cy5-2X-B-L01 (Leica 'Large' set)$1,255In StockAdd To Cart
Click to expand/collapse details Cy3/Cy5-2X-B-S01 (Sutter threaded set)$1,295Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details Cy3/Cy5-2X-B-NQF (set mounted in cube)$1,650In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details Cy3/Cy5-2X-B-NTE (set mounted in cube)$1,650In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details Cy3/Cy5-2X-B-OMF (set mounted in cube)$1,690In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details Cy3/Cy5-2X-B-OFF (set mounted in cube)$1,690In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details Cy3/Cy5-2X-B-ZHE (set mounted in cube)$1,530In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details Cy3/Cy5-2X-B-LDMK (set mounted in cube)$1,605In StockAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details Cy3/Cy5-2X-B-LDMP (set mounted in cube)$1,690In StockAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Yellow
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425683-Star
6-carboxyrhodamine 6G5255551-Star
6-JOE5205481-Star
Alexa Fluor® 5145185431-Star
Alexa Fluor® 5325345531-Star
Alexa Fluor® 5465565721-Star
Alexa Fluor® 5555535655-Star
Amplex UltraRed peroxidation product (pH 7.5)5685811-Star
ATTO 5205165381-Star
ATTO 5325015451-Star
ATTO 5505535763-Star
ATTO 5655635921-Star
BODIPY TMR-X5445733-Star
BODIPY TR-X (MeOH)5445733-Star
Calcium Orange5896093-Star
Cy3.5™5785911-Star
Cy3™5545665-Star
Dendra2 (Red)5545693-Star
DiI5515693-Star
DsRed5595835-Star
DsRed-Express5565841-Star
dTomato5565823-Star
DY-5305375603-Star
DyLight 5495535693-Star
Eosin5255461-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276171-Star
FluoSpheres Orange fluorescent microspheres5405593-Star
Gold 5255215511-Star
Gold 5505515643-Star
HiLyte Fluor™ 5555505663-Star
KFP-Red5755991-Star
LOLO-15685801-Star
Magnesium Orange5505743-Star
mApple5665941-Star
Merocyanine 5405595791-Star
MitoTracker™ Orange5515753-Star
mKO5495593-Star
mOrange5465623-Star
mOrange25515653-Star
mRFP5555833-Star
mRuby5586051-Star
mStrawberry5745961-Star
mTangerine5685851-Star
Nile red-triglyceride5105831-Star
OFP5475673-Star
Orange 5525525743-Star
pHrodo™, succinimidyl ester5605871-Star
POPO-35335743-Star
PO-PRO-35395703-Star
Pro-Q Diamond5565831-Star
Qdot® 565 Nanocrystals3005641-Star
Qdot® 585 Nanocrystals3005881-Star
Resorufin5715841-Star
Rhod-25535773-Star
Rhodamine phalloidin5585751-Star
R-phycoerythrin (PE)5655761-Star
SNARF-1 488nm (ph 6.0)5485863-Star
SNARF-1 514nm (ph 6.0)5495873-Star
SpectrumGold5305561-Star
SpectrumOrange5545873-Star
SYTO 825405593-Star
SYTOX Orange5475703-Star
Tetramethylrhodamine dextran5545823-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TRITC (Tetramethylrhodamine)5525783-Star
TurboRFP5535733-Star
VIC5305501-Star
Vybrant DyeCycle Orange5195633-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxynapthofluorescein (pH 10)5556151-Star
Alexa Fluor® 6336316471-Star
Alexa Fluor® 6476536685-Star
Alexa Fluor® 6606636915-Star
Alexa Fluor® 6806797021-Star
APC6516603-Star
APC (Allophycocyanin)6516603-Star
ATTO 6106146341-Star
ATTO 6206186421-Star
ATTO 6336296581-Star
ATTO 6356356581-Star
ATTO 6476446703-Star
ATTO 647N6456683-Star
ATTO 6556636831-Star
ATTO 6806807001-Star
BODIPY 650/665-X6466643-Star
Carboxynaphthofluorescein5996741-Star
Cy5.5™6726901-Star
Cy5™6496665-Star
DDAO6486571-Star
DiD (DiIC18(5)) - Lipophilic Tracer6486703-Star
DY-6306346561-Star
DyLight 6496516733-Star
DyLight 6806767051-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Dark Red fluorescent microspheres6566831-Star
HCS CellMask Red cytoplasmic6226441-Star
HCS LipidTOX Deep Red neutral lipid stain6356521-Star
HiLyte Fluor™ 6476496733-Star
LDS 7515607111-Star
LIVE-DEAD Fixable Far Red Dead Cell Stain6536683-Star
MitoTracker™ Deep Red6416623-Star
Nile blue-EtOH6316603-Star
Qdot® 705 Nanocrystals3007021-Star
Red 6506496653-Star
SpectrumFRed (Far Red)6506735-Star
SYTO 596226441-Star
SYTO 606506813-Star
SYTO 616186501-Star
SYTOX Red-DNA6406581-Star
TO-PRO-36426571-Star
TOTO-36426613-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



Cy3/Cy5-2X-B Cy3 Cy5 Cy3/Cy5 Pinkel Dual-Band FF560/659-Di01 FF02-628/40 FF02-534/30 FF01-577/690 FF560/659 628/40 534/30 577/690 560/659 Filter Filters Set Sets Dual 2X Cy3/Cy5-2X-B-000 Cy3/Cy5-2X-B-L01 Cy3/Cy5-2X-B-S01 Cy3/Cy5-2X-B-NQF Cy3/Cy5-2X-B-NTE Cy3/Cy5-2X-B-OMF Cy3/Cy5-2X-B-OFF Cy3/Cy5-2X-B-ZHE Cy3/Cy5-2X-B-LDMP Cy3/Cy5-2X-B-LDMK

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