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BrightLine® Sedat filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: DA/FI/TR/Cy5-4X4M-C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Semrock Filter Set: DA/FI/TR/Cy5-4X4M-C

BrightLine® Sedat filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores

This 9-filter "Sedat" quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). The complete set is comprised of a quad-band beamsplitter with four single-band emitters and four "no burn-out" single-band exciters. The single-band filters are intended to be mounted in filter wheels.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-000 (Standard size set, fits most microscopes2)$2,565In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-387/11-25$305
Single Band Exciter25 mm x 5.0 mmFF02-485/20-25$305
Single Band Exciter25 mm x 5.0 mmFF01-560/25-25$355
Single Band Exciter25 mm x 5.0 mmFF01-650/13-25$305
Single Band Emitter25 mm x 3.5 mmFF01-440/40-25$355
Single Band Emitter25 mm x 3.5 mmFF01-525/30-25$305
Single Band Emitter25 mm x 3.5 mmFF01-607/36-25$305
Single Band Emitter25 mm x 5.0 mmFF02-684/24-25$305
Quad Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF410/504/582/669-Di01-25x36$515
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-L01 (Leica 'Large' set)$2,565Ships in 2 daysAdd To Cart
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-S01 (Sutter threaded set)$2,725Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-NQF (set mounted in cube)$2,960In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-NTE (set mounted in cube)$2,960In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-OMF (set mounted in cube)$3,000In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-OFF (set mounted in cube)$3,000In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-ZHE (set mounted in cube)$2,840In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-LDMK (set mounted in cube)$2,915Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details DA/FI/TR/Cy5-4X4M-C-LDMP (set mounted in cube)$3,000In StockAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724771-Star
4-methylumbelliferone3574411-Star
7-hydroxy-4-methylcoumarin3604421-Star
Alexa Fluor® 4054014221-Star
AMCA (Aminomethylcoumarin)3504485-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804405-Star
Calcofluor White3514441-Star
Cascade Blue™4014191-Star
CellTrace calcein violet4004523-Star
DAPI3594615-Star
DiFMU-pH 9.03584501-Star
DyLight 4053984203-Star
HiLyte Fluor™ 4054034281-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
LysoSensor Blue3744243-Star
LysoTracker Blue3734221-Star
Marina Blue3634611-Star
Pacific Blue™4044553-Star
sgBFP™3874513-Star
Sirius3554241-Star
SpectrumBlue4054491-Star
TagBFP4024571-Star
Vybrant DyeCycle Violet3694371-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005251-Star
5-FAM (5-carboxyfluorescein)4925183-Star
Acridine Orange (+DNA)5025261-Star
Alexa Fluor® 4884995205-Star
Alexa Fluor® 5005035251-Star
AmCyan14584891-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025283-Star
BODIPY FL5055121-Star
BODIPY FL-MeOH5025111-Star
Calcein4945143-Star
Calcium Green-15075291-Star
CFP24905103-Star
Cy2™4925071-Star
CyQUANT GR-DNA5025231-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915073-Star
DiO4755003-Star
Dronpa5025161-Star
DY-505-Phalloidin5035301-Star
ecliptic pHluorin pH5.54735073-Star
Emerald4915113-Star
FITC (Fluorescein)4955195-Star
FlAsH-CCPFCC5115301-Star
Fluo-35065271-Star
Fluo-44945163-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895173-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
GFP (EGFP)4895115-Star
Green 4964965203-Star
Green 5005015243-Star
HCS LipidTOX Green neutral lipid stain4985073-Star
HCS LipidTOX Green phospholipidosis5045361-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoSensor Green4485021-Star
LysoTracker Green5015093-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075311-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905123-Star
mWasabi4935093-Star
NBD-X (MeOH)4675381-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135321-Star
PicoGreen®5025221-Star
PKH674885001-Star
Qdot® 525 Nanocrystals3005251-Star
ratiometric pHluorin pH55005093-Star
Rhodamine 1104975193-Star
Rhodamine 1235075291-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
sgGFP™ (super glow GFP)4725063-Star
Sodium Green5075321-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485063-Star
SYTO 164895203-Star
SYTO 94835001-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboGFP4825031-Star
Vybrant DyeCycle Green5065341-Star
wtGFP (wild type GFP, non-UV excitation)4745093-Star
YFP (yellow GFP)5135301-Star
YO-PRO-14915063-Star
YOYO-14915083-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Orange
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825953-Star
5-ROX (carboxy-X-rhodamine)5786043-Star
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425681-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5465565721-Star
Alexa Fluor® 5555535651-Star
Alexa Fluor® 5685796033-Star
Alexa Fluor® 5945906181-Star
Amplex UltraRed peroxidation product (pH 7.5)5685811-Star
AsRed 25765921-Star
ATTO 5505535761-Star
ATTO 5655635923-Star
ATTO 5905946241-Star
BOBO™-35706053-Star
BODIPY TMR-X5445731-Star
BODIPY TR-X (MeOH)5445731-Star
BODIPY TR-X phallacidin5906211-Star
Calcium Crimson5896091-Star
Calcium Orange5896091-Star
CellTrace BODIPY TR methyl ester5986251-Star
CellTracker Red CMTPX5866141-Star
Cy3.5™5785913-Star
Dendra2 (Red)5545691-Star
DiI5515691-Star
DsRed5595833-Star
DsRed-Express5565843-Star
dTomato5565823-Star
DY-5905825991-Star
DyLight 5945936181-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276171-Star
FluoSpheres Red fluorescent microspheres5776073-Star
HcRed15886181-Star
HCS LipidTOX Red neutral lipid stain5826161-Star
HCS LipidTOX Red phospholipidosis5846081-Star
HiLyte Fluor™ 5945926161-Star
KFP-Red5755993-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956131-Star
LOLO-15685801-Star
LysoTracker Red5735923-Star
Magnesium Orange5505741-Star
mApple5665943-Star
mCherry5876101-Star
Merocyanine 5405595791-Star
MitoTracker™ Orange5515751-Star
MitoTracker™ Red5785983-Star
mKate25886331-Star
mOrange5465621-Star
mOrange25515651-Star
mRFP5555833-Star
mRFP15847001-Star
mRuby5586053-Star
mStrawberry5745963-Star
mTangerine5685853-Star
Nile red-phospolipid5536371-Star
Nile red-triglyceride5105831-Star
OFP5475671-Star
Orange 5525525741-Star
pHrodo™, succinimidyl ester5605873-Star
POPO-35335741-Star
Propidium Iodide (PI)3056171-Star
Pro-Q Diamond5565833-Star
Qdot® 585 Nanocrystals3005883-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
ReAsH-CCPGCC5926061-Star
Red 5805806031-Star
Resorufin5715843-Star
Rhod-25535771-Star
Rhodamine phalloidin5585751-Star
Rhodamine Red-X5735913-Star
SNARF (carboxy) 514 Excitation pH95766381-Star
SNARF-1 488nm (ph 6.0)5485863-Star
SNARF-1 488nm (ph 9.0)5766381-Star
SNARF-1 514nm (ph 6.0)5495873-Star
SNARF-1 514nm (ph 9.0)5766381-Star
SpectrumOrange5545873-Star
Sulforhodamine 101-EtOH5785933-Star
SYTOX Orange5475701-Star
Tetramethylrhodamine dextran5545821-Star
Texas Red dextran5926131-Star
Texas Red DHPE5846081-Star
Texas Red®5926141-Star
TRITC (Tetramethylrhodamine)5525783-Star
TurboFP635(Katushka)5916381-Star
TurboRFP5535731-Star
X-Rhod-1 Indicator5806011-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 6476536683-Star
Alexa Fluor® 6606636913-Star
Alexa Fluor® 6806797021-Star
APC6516601-Star
APC (Allophycocyanin)6516601-Star
ATTO 6356356581-Star
ATTO 6476446703-Star
ATTO 647N6456683-Star
ATTO 6556636833-Star
ATTO 6806807001-Star
BODIPY 650/665-X6466641-Star
Cy5.5™6726901-Star
Cy5™6496663-Star
DDAO6486571-Star
DiD (DiIC18(5)) - Lipophilic Tracer6486703-Star
DY-6306346561-Star
DY-6756756991-Star
DyLight 6496516733-Star
DyLight 6806767051-Star
FluoSpheres Dark Red fluorescent microspheres6566833-Star
HiLyte Fluor™ 6476496733-Star
LIVE-DEAD Fixable Far Red Dead Cell Stain6536683-Star
MitoTracker™ Deep Red6416621-Star
Nile blue-EtOH6316601-Star
Red 6506496653-Star
SpectrumFRed (Far Red)6506733-Star
SYTO 606506813-Star
SYTOX Red-DNA6406581-Star
TO-PRO-36426571-Star
TOTO-36426611-Star




DA/FI/TR/Cy5-4X4M-C DA/FI/TR/Cy5 DAPI/FITC/TRITC/Cy5 DAPI FITC TRITC Cy5 Sedat 410/504/582/669 387/11 485/20 560/25 650/13 440/40 525/30 607/36 684/24 Quad-Band FF410/504/582/669-Di01 FF02-485/20 FF02-684/24 FF01-525/30 FF01-560/25 FF01-607/36 FF01-650/13 FF01-440/40 FF01-387/11 Filter Filters Set Sets Quad 4X4M DA/FI/TR/Cy5-4X4M-C-000 DA/FI/TR/Cy5-4X4M-C-L01 DA/FI/TR/Cy5-4X4M-C-S01 DA/FI/TR/Cy5-4X4M-C-NQF DA/FI/TR/Cy5-4X4M-C-NTE DA/FI/TR/Cy5-4X4M-C-OMF DA/FI/TR/Cy5-4X4M-C-OFF DA/FI/TR/Cy5-4X4M-C-ZHE DA/FI/TR/Cy5-4X4M-C-LDMP DA/FI/TR/Cy5-4X4M-C-LDMK

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