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BrightLine® Sedat filter set, optimized for Cyan & Yellow Fluorescent Proteins & HcRed and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: CFP/YFP/HcRed-3X3M-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: CFP/YFP/HcRed-3X3M-B

BrightLine® Sedat filter set, optimized for Cyan & Yellow Fluorescent Proteins & HcRed and other like fluorophores

This 7-filter "Sedat" set is ideal for high-speed sequential imaging CFP, YFP, and HcRed with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The set is also ideal for samples triple-labeled with CFP, YFP, and an orange or red dye, like Texas Red. It is comprised of three single-band exciters and three single-band emitters, along with a triple-band beamsplitter, and thus works best when used with both exciter and emitter filter wheels.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-000 (Standard size set, fits most microscopes2)$2,075In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-427/10-25$355
Single Band Exciter25 mm x 5.0 mmFF01-504/12-25$305
Single Band Exciter25 mm x 5.0 mmFF01-589/15-25$355
Single Band Emitter25 mm x 5.0 mmFF02-472/30-25$305
Single Band Emitter25 mm x 3.5 mmFF01-542/27-25$305
Single Band Emitter25 mm x 5.0 mmFF02-632/22-25$305
Triple Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF444/521/608-Di01-25x36$465
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-L01 (Leica 'Large' set)$2,075Ships in 2 daysAdd To Cart
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-S01 (Sutter threaded set)$2,195Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-NQF (set mounted in cube)$2,470In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-NTE (set mounted in cube)$2,470In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-OMF (set mounted in cube)$2,510In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-OFF (set mounted in cube)$2,510In StockAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-ZHE (set mounted in cube)$2,350In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-LDMK (set mounted in cube)$2,425Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details CFP/YFP/HcRed-3X3M-B-LDMP (set mounted in cube)$2,510Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Cyan
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Aqua 4314304783-Star
ATTO 4254364843-Star
BD Horizon V4504064491-Star
BD Horizon V5004105001-Star
BOBO™-14614845-Star
Cerulean4344733-Star
CFP (ECFP)4334755-Star
DEAC4324723-Star
DY-4154184703-Star
ECFP4354753-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034551-Star
mBBr+GSH3944901-Star
mTFP14624921-Star
Pacific Blue™4044551-Star
POPO-14334573-Star
PO-PRO-14334563-Star
SpectrumAqua4344813-Star
SYTO 454514851-Star
SYTOX Blue4444705-Star
TagBFP4024571-Star
TagCFP4584801-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Yellow
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925181-Star
6-carboxyrhodamine 6G5255551-Star
6-JOE5205481-Star
Acridine Orange (+DNA)5025263-Star
Alexa Fluor® 4884995201-Star
Alexa Fluor® 5005035253-Star
Alexa Fluor® 5145185431-Star
Alexa Fluor® 5325345531-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
ATTO 5205165381-Star
ATTO 5325015451-Star
BCECF (pH 5.5)4815181-Star
BCECF (pH 9.0)5025283-Star
BODIPY R6G5285471-Star
Calcein4945141-Star
Calcium Green-15075295-Star
CyQUANT GR-DNA5025233-Star
DAF-FM-NO4955191-Star
Dronpa5025161-Star
DY-505-Phalloidin5035303-Star
DyLight 4884935181-Star
Eosin5255461-Star
FITC (Fluorescein)4955191-Star
FlAsH-CCPFCC5115303-Star
Fluo-35065275-Star
Fluo-44945161-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895171-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
FM 1-434735791-Star
Gold 5255215511-Star
Green 4964965201-Star
Green 5005015243-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975263-Star
JO-JO-1, JO-PRO-15305441-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075313-Star
mHoneyDew4785623-Star
MitoTracker™ Green4905121-Star
mWasabi4935091-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135323-Star
PicoGreen®5025223-Star
Qdot® 525 Nanocrystals3005253-Star
Qdot® 545 Nanocrystals3005431-Star
Rhodamine 1104975191-Star
Rhodamine 1235075295-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
Sodium Green5075323-Star
SpectrumGreen4975383-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095263-Star
SYTO 115065253-Star
SYTO 164895201-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TO-PRO-15155313-Star
TOTO-15145313-Star
TurboYFP5255381-Star
Venus5165283-Star
VIC5305501-Star
Vybrant DyeCycle Green5065343-Star
Vybrant DyeCycle Orange5195631-Star
YFP (yellow GFP)5135305-Star
YO-PRO-14915061-Star
YOYO-14915081-Star
ZsYellow15295391-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825951-Star
5-ROX (carboxy-X-rhodamine)5786041-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5685796031-Star
Alexa Fluor® 5945906185-Star
ATTO 5905946243-Star
ATTO 5946016243-Star
ATTO 6106146341-Star
BOBO™-35706051-Star
BODIPY 630/650-X6256411-Star
BODIPY TR-X phallacidin5906213-Star
Calcium Crimson5896091-Star
CellTrace BODIPY TR methyl ester5986253-Star
CellTracker Red CMTPX5866141-Star
DyLight 5945936183-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Red fluorescent microspheres5776071-Star
HcRed15886183-Star
HCS CellMask Red cytoplasmic6226441-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846081-Star
HiLyte Fluor™ 5945926163-Star
Hypericin6006031-Star
KFP-Red5755991-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956131-Star
mCherry5876101-Star
MitoTracker™ Red5785981-Star
mKate25886333-Star
mPlum5896493-Star
mRFP15847001-Star
Nile red-phospolipid5536371-Star
Qdot® 625 Nanocrystals3006211-Star
ReAsH-CCPGCC5926061-Star
Red 5805806031-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 9.0)5766383-Star
SpectrumRed5876153-Star
SYTO 176196381-Star
SYTO 596226441-Star
SYTO 616186501-Star
Texas Red dextran5926133-Star
Texas Red DHPE5846081-Star
Texas Red®5926143-Star
TurboFP635(Katushka)5916383-Star
X-Rhod-1 Indicator5806011-Star
YO-PRO-36136291-Star
YOYO-36116311-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
Multiband Filter Set TerminologyCompares benefits and drawbacks of full multiband, pinkel and sedat filter sets
Journal Article
TopicDescription
New Optical Filters Improve High-Speed Multicolor Fluorescence ImagingTuran Erdogan, PhD, Semrock Inc., BioPhotonics, March 2006
Compares benefits and drawbacks of full multiband, pinkel and sedat filter sets for high-speed multicolor fluorescence imaging
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.



CFP/YFP/HcRed-3X3M-B CFP/YFP/HcRed CFP YFP HcRed Sedat Triple-Band FF444/521/608-Di01 FF02-632/22 FF01-504/12 FF01-427/10 FF02-472/30 FF01-542/27 FF01-589/15 FF444/521/608 444/521/608 632/22 504/12 427/10 472/30 542/27 589/15 Filter Filters Set Sets Triple 3X3M CFP/YFP/HcRed-3X3M-B-000 CFP/YFP/HcRed-3X3M-B-L01 CFP/YFP/HcRed-3X3M-B-S01 CFP/YFP/HcRed-3X3M-B-NQF CFP/YFP/HcRed-3X3M-B-NTE CFP/YFP/HcRed-3X3M-B-OMF CFP/YFP/HcRed-3X3M-B-OFF CFP/YFP/HcRed-3X3M-B-ZHE CFP/YFP/HcRed-3X3M-B-LDMP CFP/YFP/HcRed-3X3M-B-LDMK

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