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BrightLine® single-band filter set, optimized for Yellow Fluorescent Protein and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: YFP-2427B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: YFP-2427B

BrightLine® single-band filter set, optimized for Yellow Fluorescent Protein and other like fluorophores

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring YFP and its variants – no more compromising or exchanging filter sets for different YFP experiments.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details YFP-2427B-000 (Standard size set, fits most microscopes2)$785In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-500/24-25$305
Single Band Emitter25 mm x 3.5 mmFF01-542/27-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF520-Di02-25x36$255
Click to expand/collapse details YFP-2427B-000-ZERO (Standard size -ZERO pixel set)$884In StockAdd To Cart
Click to expand/collapse details YFP-2427B-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details YFP-2427B-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details YFP-2427B-NQF (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details YFP-2427B-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details YFP-2427B-NTE (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details YFP-2427B-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details YFP-2427B-OMF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details YFP-2427B-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details YFP-2427B-OFF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details YFP-2427B-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details YFP-2427B-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details YFP-2427B-ZHE (set mounted in cube)$1,060In StockAdd To Cart
Click to expand/collapse details YFP-2427B-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details YFP-2427B-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details YFP-2427B-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details YFP-2427B-AMF-ZERO (-ZERO pixel set mounted in cube)$1,349Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-FAM (5-carboxyfluorescein)4925181-Star
6-carboxyrhodamine 6G5255551-Star
Acridine Orange (+DNA)5025261-Star
Alexa Fluor® 4884995201-Star
Alexa Fluor® 5005035251-Star
Alexa Fluor® 5145185433-Star
Alexa Fluor® 5325345531-Star
ATTO 4885005251-Star
ATTO 4954955271-Star
ATTO 5205165381-Star
BCECF (pH 5.5)4815181-Star
BCECF (pH 9.0)5025281-Star
BODIPY R6G5285471-Star
Calcium Green-15075295-Star
CyQUANT GR-DNA5025231-Star
FITC (Fluorescein)4955191-Star
Fluorescein dextran5015241-Star
Fluorescein-pH 8.04895171-Star
FluoSpheres Orange fluorescent microspheres5405591-Star
FluoSpheres Yellow-Green fluorescent microspheres5035141-Star
FM 1-434735791-Star
Gold 5255215511-Star
Green 4964965201-Star
Green 5005015241-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975261-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985251-Star
LysoTracker Yellow HCK-1234885653-Star
Magnesium Green5075311-Star
NBD-X (MeOH)4675381-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
Nile red-triglyceride5105831-Star
Oregon Green™ 4884985261-Star
Oregon Green™ 5145135323-Star
Qdot® 545 Nanocrystals3005431-Star
Qdot® 565 Nanocrystals3005641-Star
Rhodamine 1104975191-Star
Rhodamine 1235075295-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
Sodium Green5075323-Star
SYBR Gold nucleic acid gel stain-DNA4965393-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYBR Safe DNA gel stain-DNA5095261-Star
SYTO 115065251-Star
SYTO 134485061-Star
SYTO 164895201-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
Vybrant DyeCycle Green5065343-Star
Vybrant DyeCycle Orange5195631-Star
YFP (yellow GFP)5135305-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.

YFP-2427B YFP Venus 500/24 542/27 520 Single-Band FF01-500/24 FF01-542/27 FF520-Di02 FF520 Filter Filters Set Sets Alexa-Fluor-514 AF514 Alexa514 Calcium-Green-1 Eosin Fluo-3 HCS-LipidTOX-Green-phospholipidosis LysoTracker-Yellow-HCK-123 mHoneyDew Oregon-Green-514 Rhodamine-123 Sodium-Green Vybrant-DyeCycle-Green yellow-GFP YFP-2427B-000 YFP-2427B-000-ZERO YFP-2427B-L01-ZERO YFP-2427B-U02 YFP-2427B-NQF YFP-2427B-NQF-ZERO YFP-2427B-NTE YFP-2427B-NTE-ZERO YFP-2427B-OMF YFP-2427B-OMF-ZERO YFP-2427B-OFF YFP-2427B-OFF-ZERO YFP-2427B-OFX YFP-2427B-ZHE YFP-2427B-ZHE-ZERO YFP-2427B-LDMP-ZERO YFP-2427B-LDMK-ZERO YFP-2427B-AMF-ZERO

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