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BrightLine® single-band filter set, optimized for Texas Red and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: TxRed-4040C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: TxRed-4040C

BrightLine® single-band filter set, optimized for Texas Red and other like fluorophores

This filter set is ideal for use with Texas Red® and other red fluorophores, including the monomeric fluorescent protein mCherry. It also works well for broader orange-to-red fluorophores like MitoTracker Red. It is optimized to provide the highest brightness, while maintaining a very high signal-to-noise ratio.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details TxRed-4040C-000 (Standard size set, fits most microscopes2)$825In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-562/40-25$325
Single Band Emitter25 mm x 3.5 mmFF01-624/40-25$325
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF593-Di03-25x36$275
Click to expand/collapse details TxRed-4040C-000-ZERO (Standard size -ZERO pixel set)$924In StockAdd To Cart
Click to expand/collapse details TxRed-4040C-L01-ZERO (Leica 'Large' -ZERO pixel set)$924Ships in 2 daysAdd To Cart
Click to expand/collapse details TxRed-4040C-U02 (Olympus large-size (FX) set for U-M710I)$1,370Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details TxRed-4040C-NQF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details TxRed-4040C-NQF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details TxRed-4040C-NTE (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details TxRed-4040C-NTE-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details TxRed-4040C-OMF (set mounted in cube)$1,260In StockAdd To Cart
Click to expand/collapse details TxRed-4040C-OMF-ZERO (-ZERO pixel set mounted in cube)$1,359In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details TxRed-4040C-OFF (set mounted in cube)$1,260In StockAdd To Cart
Click to expand/collapse details TxRed-4040C-OFF-ZERO (-ZERO pixel set mounted in cube)$1,359In StockAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details TxRed-4040C-OFX (set mounted in cube)$2,065Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details TxRed-4040C-ZHE (set mounted in cube)$1,100In StockAdd To Cart
Click to expand/collapse details TxRed-4040C-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,199In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details TxRed-4040C-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,274Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details TxRed-4040C-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,359In StockAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details TxRed-4040C-AMF-ZERO (-ZERO pixel set mounted in cube)$1,389In StockAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-ROX (carboxy-X-rhodamine)5786045-Star
7-AAD (7-aminoactinomycin D)5486481-Star
Alexa Fluor® 5685796035-Star
Alexa Fluor® 5945906185-Star
Amplex UltraRed peroxidation product (pH 7.5)5685811-Star
AsRed 25765923-Star
ATTO 5655635921-Star
ATTO 5905946241-Star
ATTO 5946016241-Star
BODIPY TR-X phallacidin5906211-Star
Calcium Crimson5896095-Star
CellTrace BODIPY TR methyl ester5986251-Star
CellTracker Red CMTPX5866143-Star
DyLight 5945936181-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276171-Star
FluoSpheres Red fluorescent microspheres5776073-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846083-Star
HiLyte Fluor™ 5945926161-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956131-Star
LysoTracker Red5735921-Star
MitoTracker™ Red5785985-Star
Nile red-phospolipid5536373-Star
pHrodo™, succinimidyl ester5605871-Star
Propidium Iodide (PI)3056173-Star
Pro-Q Diamond5565831-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Red 5805806033-Star
Rhodamine Red-X5735911-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 6.0)5485861-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 6.0)5495871-Star
SNARF-1 514nm (ph 9.0)5766383-Star
Sulforhodamine 101-EtOH5785931-Star
Tetramethylrhodamine dextran5545821-Star
Texas Red dextran5926131-Star
Texas Red DHPE5846083-Star
Texas Red®5926145-Star
X-Rhod-1 Indicator5806013-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.

TxRed-4040C TxRed TexasRed 593 562/40 624/40 Single-Band FF593-Di03 FF01-562/40 FF01-624/40 FF593 5-ROX carboxy-X-rhodamine Alexa-Fluor-568 AF568 Alexa568 Alexa-Fluor-594 AF594 Alexa594 AsRed-2 Bobo-3 Calcium-Crimson CellTracker-Red-CMTPX Cy3.5 DY-590 FluoSpheres-Red KFP-Red mCherry MitoTracker-Red mRFP1 mRuby Nile-red-phospolipid Propidium-Iodide PI Red-580 Texas-Red-DHPE SNARF-1-488nm-(ph9.0) SNARF-1-514nm-(ph9.0) TxRed-4040C-000 TxRed-4040C-000-ZERO TxRed-4040C-L01-ZERO TxRed-4040C-U02 TxRed-4040C-NQF TxRed-4040C-NQF-ZERO TxRed-4040C-NTE TxRed-4040C-NTE-ZERO TxRed-4040C-OMF TxRed-4040C-OMF-ZERO TxRed-4040C-OFF TxRed-4040C-OFF-ZERO TxRed-4040C-OFX TxRed-4040C-ZHE TxRed-4040C-ZHE-ZERO TxRed-4040C-LDMP-ZERO TxRed-4040C-LDMK-ZERO TxRed-4040C-AMF-ZERO

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