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BrightLine® single-band filter set, optimized for Spectrum Green and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: SpGr-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: SpGr-B

BrightLine® single-band filter set, optimized for Spectrum Green and other like fluorophores

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Spectrum Green.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details SpGr-B-000 (Standard size set, fits most microscopes2)$785Ships in 2 daysAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-494/20-25$405
Single Band Emitter25 mm x 3.5 mmFF01-527/20-25$355
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF506-Di03-25x36$255
Click to expand/collapse details SpGr-B-000-ZERO (Standard size -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details SpGr-B-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details SpGr-B-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details SpGr-B-NQF (set mounted in cube)$1,180Ships in 2 daysAdd To Cart
Click to expand/collapse details SpGr-B-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details SpGr-B-NTE (set mounted in cube)$1,180Ships in 2 daysAdd To Cart
Click to expand/collapse details SpGr-B-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details SpGr-B-OMF (set mounted in cube)$1,220Ships in 2 daysAdd To Cart
Click to expand/collapse details SpGr-B-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details SpGr-B-OFF (set mounted in cube)$1,220Ships in 2 daysAdd To Cart
Click to expand/collapse details SpGr-B-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details SpGr-B-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details SpGr-B-ZHE (set mounted in cube)$1,060Ships in 2 daysAdd To Cart
Click to expand/collapse details SpGr-B-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details SpGr-B-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details SpGr-B-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,319Ships in 2 daysAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details SpGr-B-AMF-ZERO (-ZERO pixel set mounted in cube)$1,349Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-carboxy-2,7-dichlorofluorescein5005253-Star
5-FAM (5-carboxyfluorescein)4925183-Star
Acridine Orange (+DNA)5025263-Star
Alexa Fluor® 4884995205-Star
Alexa Fluor® 5005035253-Star
Alexa Fluor® 5145185431-Star
ATTO 4885005253-Star
ATTO 4954955273-Star
AzamiGreen4945051-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025283-Star
BODIPY FL5055123-Star
BODIPY FL-MeOH5025113-Star
Calcein4945143-Star
Calcium Green-15075293-Star
CFP24905101-Star
Cy2™4925071-Star
CyQUANT GR-DNA5025233-Star
DAF-FM-NO4955193-Star
Dendra2 (Green)4915071-Star
DiO4755001-Star
Dronpa5025163-Star
DY-505-Phalloidin5035303-Star
Emerald4915113-Star
FITC (Fluorescein)4955195-Star
FlAsH-CCPFCC5115301-Star
Fluo-35065273-Star
Fluo-44945163-Star
Fluorescein dextran5015243-Star
Fluorescein-pH 8.04895173-Star
Fluoro-Emerald4945183-Star
FluoSpheres Yellow-Green fluorescent microspheres5035143-Star
GFP (EGFP)4895113-Star
Green 4964965203-Star
Green 5005015243-Star
HCS LipidTOX Green neutral lipid stain4985071-Star
HCS LipidTOX Green phospholipidosis5045363-Star
HiLyte Fluor™ 4884975263-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985253-Star
LysoTracker Green5015091-Star
LysoTracker Yellow HCK-1234885651-Star
Magnesium Green5075313-Star
mHoneyDew4785621-Star
MitoTracker™ Green4905123-Star
mWasabi4935091-Star
NBD-X (MeOH)4675381-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955243-Star
Oregon Green™ 4884985263-Star
Oregon Green™ 5145135321-Star
PicoGreen®5025223-Star
PKH674885001-Star
Qdot® 525 Nanocrystals3005251-Star
ratiometric pHluorin pH55005091-Star
Rhodamine 1104975193-Star
Rhodamine 1235075293-Star
Rhodamine Green4975233-Star
Rhodol Green4975243-Star
Sodium Green5075323-Star
SpectrumGreen4975385-Star
SYBR Gold nucleic acid gel stain-DNA4965391-Star
SYBR Green I nucleic acid gel stain-DNA4985223-Star
SYBR Safe DNA gel stain-DNA5095263-Star
SYTO 115065253-Star
SYTO 134485061-Star
SYTO 164895203-Star
SYTO 94835001-Star
SYTO RNASelect geen fluorescent cell stain5035273-Star
SYTOX Green-DNA5045243-Star
TO-PRO-15155311-Star
TOTO-15145311-Star
TurboGFP4825031-Star
Venus5165281-Star
Vybrant DyeCycle Green5065343-Star
YFP (yellow GFP)5135301-Star
YO-PRO-14915061-Star
YOYO-14915081-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
FISH (Fluorescence in Situ Hybridization)An overview of FISH, including solutions from Semrock and additional learning resources.



SpGr-B SpectrumGreen SpGreen SpGr FISH 494/20 527/20 506 Single-Band FF506-Di03 FF01-527/20 FF01-494/20 FF506 Filter Filters Set Sets 5-FAM Acridine-Orange-(+DNA) Alexa-Fluor-488 AF488 Alexa488 Alexa-Fluor-500 AF500 Alexa500 ATTO-488 ATTO-495 BCECF BODIPY-FL Calcein calcium-Green-1 CyQUANT-GR-DNA Dronpa DY-505-Phalloidin Emerald FITC Fluorescein Fluo-3 Fluo-4 Fluoro-Emerald FluoSpheres-Yellow-Green GFP EGFP Green-496 Green-500 Magnesium-Green MitoTracker-Green Oregon-Green-488 Rhodamine-110 SpGr-B-000 SpGr-B-000-ZERO SpGr-B-L01-ZERO SpGr-B-U02 SpGr-B-NQF SpGr-B-NQF-ZERO SpGr-B-NTE SpGr-B-NTE-ZERO SpGr-B-OMF SpGr-B-OMF-ZERO SpGr-B-OFF SpGr-B-OFF-ZERO SpGr-B-OFX SpGr-B-ZHE SpGr-B-ZHE-ZERO SpGr-B-LDMP-ZERO SpGr-B-LDMK-ZERO SpGr-B-AMF-ZERO

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