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BrightLine® single-band filter set, optimized for Spectrum Gold and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: SpGold-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: SpGold-B

BrightLine® single-band filter set, optimized for Spectrum Gold and other like fluorophores

This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) available for measuring Spectrum Gold.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details SpGold-B-000 (Standard size set, fits most microscopes2)$785In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-534/20-25$355
Single Band Emitter25 mm x 3.5 mmFF01-572/28-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF552-Di02-25x36$255
Click to expand/collapse details SpGold-B-000-ZERO (Standard size -ZERO pixel set)$884In StockAdd To Cart
Click to expand/collapse details SpGold-B-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details SpGold-B-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details SpGold-B-NQF (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details SpGold-B-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details SpGold-B-NTE (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details SpGold-B-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details SpGold-B-OMF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details SpGold-B-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details SpGold-B-OFF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details SpGold-B-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details SpGold-B-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details SpGold-B-ZHE (set mounted in cube)$1,060In StockAdd To Cart
Click to expand/collapse details SpGold-B-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details SpGold-B-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details SpGold-B-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details SpGold-B-AMF-ZERO (-ZERO pixel set mounted in cube)$1,349Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)5425681-Star
6-carboxyrhodamine 6G5255551-Star
6-JOE5205481-Star
Alexa Fluor® 5145185431-Star
Alexa Fluor® 5325345535-Star
Alexa Fluor® 5465565723-Star
Alexa Fluor® 5555535651-Star
Amplex UltraRed peroxidation product (pH 7.5)5685811-Star
ATTO 5205165381-Star
ATTO 5325015453-Star
ATTO 5505535761-Star
BODIPY R6G5285471-Star
BODIPY TMR-X5445733-Star
BODIPY TR-X (MeOH)5445733-Star
Calcium Orange5896093-Star
Cy3.5™5785911-Star
Cy3™5545661-Star
Dendra2 (Red)5545691-Star
DiI5515691-Star
DsRed5595831-Star
DsRed-Express5565841-Star
dTomato5565821-Star
DY-5305375603-Star
DyLight 5495535691-Star
Eosin5255461-Star
Ethidium bromide5186031-Star
FluoSpheres Orange fluorescent microspheres5405593-Star
Gold 5255215513-Star
Gold 5505515641-Star
HiLyte Fluor™ 5555505663-Star
JO-JO-1, JO-PRO-15305441-Star
LOLO-15685801-Star
Magnesium Orange5505743-Star
mApple5665941-Star
Merocyanine 5405595791-Star
MitoTracker™ Orange5515751-Star
mKO5495593-Star
mOrange5465623-Star
mOrange25515653-Star
mRFP5555831-Star
mStrawberry5745961-Star
mTangerine5685851-Star
Nile red-triglyceride5105833-Star
OFP5475673-Star
Orange 5525525741-Star
pHrodo™, succinimidyl ester5605871-Star
POPO-35335743-Star
PO-PRO-35395703-Star
Pro-Q Diamond5565831-Star
Qdot® 565 Nanocrystals3005641-Star
Qdot® 585 Nanocrystals3005881-Star
Resorufin5715841-Star
Rhod-25535771-Star
Rhodamine phalloidin5585751-Star
R-phycoerythrin (PE)5655761-Star
SNARF-1 488nm (ph 6.0)5485863-Star
SNARF-1 514nm (ph 6.0)5495873-Star
SpectrumGold5305565-Star
SpectrumOrange5545871-Star
SYTO 825405593-Star
SYTOX Orange5475703-Star
Tetramethylrhodamine dextran5545821-Star
TRITC (Tetramethylrhodamine)5525785-Star
TurboRFP5535731-Star
TurboYFP5255381-Star
VIC5305501-Star
Vybrant DyeCycle Orange5195631-Star
ZsYellow15295391-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
FISH (Fluorescence in Situ Hybridization)An overview of FISH, including solutions from Semrock and additional learning resources.



SpGold-B SpectrumGold SpGold FISH 534/20 572/28 552 Single-Band FF01-572/28 FF01-534/20 FF552-Di02 FF552 Filter Filters Set Sets Alexa-Fluor-532 AF532 Alexa532 Alexa-Fluor-546 AF546 Alexa546 ATTO-532 BODIPY-TMR-X Calcium-Orange DY-530 FluoSpheres-Orange Gold-525 HiLyte-Fluor-555 Magnesium-Orange mKO mOrange mOrange2 Nile-red-triglyceride OFP POPO-3 PO-PRO-3 SYTO-82 TRITC Tetramethylrhodamine SNARF-1-488nm-(ph6.0) SNARF-1-514nm-(ph6.0) SpGold-B-000 SpGold-B-000-ZERO SpGold-B-L01-ZERO SpGold-B-U02 SpGold-B-NQF SpGold-B-NQF-ZERO SpGold-B-NTE SpGold-B-NTE-ZERO SpGold-B-OMF SpGold-B-OMF-ZERO SpGold-B-OFF SpGold-B-OFF-ZERO SpGold-B-OFX SpGold-B-ZHE SpGold-B-ZHE-ZERO SpGold-B-LDMP-ZERO SpGold-B-LDMK-ZERO SpGold-B-AMF-ZERO

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