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BrightLine® long-pass filter set, optimized for most Qdot Nanocrystals and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: QDLP-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Acridine Orange (+RNA)4606501-Star
Alexa Fluor® 4304315413-Star
ATTO 4654535071-Star
BCECF (pH 5.5)4815181-Star
BD Horizon V5004105001-Star
Cascade Yellow™3995491-Star
CellTracker Violet BMQC+GSH4065261-Star
CFP (ECFP)4334751-Star
FM 1-434735791-Star
Fura Red™ (high pH)5726573-Star
Lucifer yellow4285443-Star
LysoSensor Green4485021-Star
LysoTracker Yellow HCK-1234885651-Star
NBD-X (MeOH)4675381-Star
Pacific Orange4005511-Star
Qdot® 525 Nanocrystals3005255-Star
Qdot® 545 Nanocrystals3005431-Star
Qdot® 565 Nanocrystals3005645-Star
Qdot® 585 Nanocrystals3005885-Star
Qdot® 605 Nanocrystals3006025-Star
Qdot® 625 Nanocrystals3006215-Star
Qdot® 655 Nanocrystals3006545-Star
Qdot® 705 Nanocrystals3007025-Star
Qdot® 800 Nanocrystals3007923-Star
SYPRO Ruby protein gel stain4696313-Star
ThiolTracker Violet+GSH4045261-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Laser Damage ThresholdProvides information for calculating laser damage threshold for pulsed and continuous wave lasers
Revised Filter Set


BrightLine® long-pass filter set, optimized for most Qdot Nanocrystals and other like fluorophores

High brightness and contrast long-pass filter set. Ideal for use with: Qdot 525, Qdot 565, Qdot 585, Qdot 605, Qdot 625, Qdot 655, Qdot 705, Qdot 800 Nanocrystals
Part # QDLP-C-000
(Non-US pricing info)

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