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BrightLine® single-band filter set, optimized for mCherry and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: mCherry-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
2-dodecylresorufin-lipid5825951-Star
5-carboxynapthofluorescein (pH 10)5556151-Star
5-ROX (carboxy-X-rhodamine)5786043-Star
7-AAD (7-aminoactinomycin D)5486483-Star
Alexa Fluor® 5685796033-Star
Alexa Fluor® 5945906181-Star
AsRed 25765923-Star
ATTO 5655635921-Star
ATTO 5905946241-Star
ATTO 5946016241-Star
BOBO™-35706053-Star
BODIPY TR-X phallacidin5906211-Star
Calcium Crimson5896091-Star
Carboxynaphthofluorescein5996741-Star
CellTrace BODIPY TR methyl ester5986251-Star
CellTracker Red CMTPX5866143-Star
ChlorophyllA4806501-Star
Cy3.5™5785911-Star
DDAO6486571-Star
DsRed5595831-Star
DsRed-Express5565841-Star
dTomato5565821-Star
DY-5905825993-Star
DyLight 5945936181-Star
Ethidium bromide5186031-Star
Ethidium homodimer5276171-Star
FluoSpheres Crimson fluorescent microspheres6206461-Star
FluoSpheres Red fluorescent microspheres5776073-Star
HcRed15886181-Star
HCS LipidTOX Red neutral lipid stain5826163-Star
HCS LipidTOX Red phospholipidosis5846083-Star
HiLyte Fluor™ 5945926161-Star
KFP-Red5755993-Star
LIVE-DEAD Fixable Red Dead Cell Stain5956131-Star
LysoTracker Red5735921-Star
mApple5665941-Star
mCherry5876105-Star
MitoTracker™ Red5785983-Star
mKate25886331-Star
mPlum5896491-Star
mRFP5555831-Star
mRFP15847005-Star
mRuby5586053-Star
mStrawberry5745961-Star
mTangerine5685851-Star
Nile red-phospolipid5536373-Star
PerCP4906771-Star
pHrodo™, succinimidyl ester5605871-Star
Propidium Iodide (PI)3056173-Star
Qdot® 605 Nanocrystals3006021-Star
Qdot® 625 Nanocrystals3006211-Star
Qdot® 655 Nanocrystals3006541-Star
ReAsH-CCPGCC5926061-Star
Red 5805806033-Star
Rhodamine Red-X5735911-Star
SNARF (carboxy) 514 Excitation pH95766383-Star
SNARF-1 488nm (ph 6.0)5485861-Star
SNARF-1 488nm (ph 9.0)5766383-Star
SNARF-1 514nm (ph 6.0)5495871-Star
SNARF-1 514nm (ph 9.0)5766383-Star
SpectrumOrange5545871-Star
SpectrumRed5876151-Star
Sulforhodamine 101-EtOH5785931-Star
Tetramethylrhodamine dextran5545821-Star
Texas Red dextran5926131-Star
Texas Red DHPE5846083-Star
Texas Red®5926143-Star
TurboFP635(Katushka)5916381-Star
X-Rhod-1 Indicator5806013-Star
YOYO-36116311-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Fluorescence Imaging with Quantum Dot NanocrystalsDescribes how the Qdot nanocrystal structure is unique and the Semrock filter sets available for use with Qdots.
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
Revised Filter Set

mCherry-C.png

BrightLine® single-band filter set, optimized for mCherry and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: mRFP1, mCherry, mApple
Part # mCherry-C-000
$785
(Non-US pricing info)


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US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



mCherry-B

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