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BrightLine® FRET filter set, optimized for Green & Red Fluorescent Protein and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: FRET-GFP/RFP-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 4884995203-Star
Cy2™4925073-Star
FITC (Fluorescein)4955193-Star
GFP (EGFP)4895113-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Red
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Cy3™5545661-Star
DsRed5595833-Star
dTomato5565823-Star
mCherry5876103-Star
mRFP15847003-Star
mStrawberry5745963-Star
TRITC (Tetramethylrhodamine)5525781-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Fluorescence Resonance Energy TransferTHIS MAY BE REMOVED AND USED ONLY IN APPLICATIONS SECTION. Describes the FRET interaction and considerations for choosing filter sets
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
FRET (Flourescence Resonance Energy Transfer)An overview of FRET, including solutions from Semrock and additional learning resources.
Ratiometric ImagingAn overview of ratiometric imaging, including solutions from Semrock and additional learning resources.
Revised Filter Set

FRET-GFP_RFP-D.png

BrightLine® FRET filter set, optimized for Green & Red Fluorescent Protein and other like fluorophores

4-filter single-band set. Ideal for use with: GFP , EGFP, FITC , Cy2, Alexa Fluor 488, mCherry, mStrawberry, dTomato, DsRed, TRITC
Part # FRET-GFP/RFP-D-000
$1125
(Non-US pricing info)


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FRET-GFP/RFP-B

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