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BrightLine® FRET filter set, optimized for Blue & Green Fluorescent Protein and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: FRET-BFP/GFP-B-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: FRET-BFP/GFP-B

BrightLine® FRET filter set, optimized for Blue & Green Fluorescent Protein and other like fluorophores

This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for blue donor fluorescence and green acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details FRET-BFP/GFP-B-000 (Standard size set, fits most microscopes2)$1,125In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-387/11-25$305
Single Band Emitter25 mm x 3.5 mmFF02-447/60-25$305
Single Band Emitter25 mm x 3.5 mmFF01-520/35-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF409-Di03-25x36$255
Click to expand/collapse details FRET-BFP/GFP-B-000-ZERO (Standard size -ZERO pixel set)$1,224Ships in 2 daysAdd To Cart
Click to expand/collapse details FRET-BFP/GFP-B-L01-ZERO (Leica 'Large' -ZERO pixel set)$1,224Ships in 2 daysAdd To Cart
Click to expand/collapse details FRET-BFP/GFP-B-S01 (Sutter threaded set)$1,165Ships in 2 daysAdd To Cart
Click to expand/collapse details FRET-BFP/GFP-B-S01-ZERO (Sutter threaded set -ZERO pixel set)$1,264Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details FRET-BFP/GFP-B-NQF (set mounted in cube)$1,520In StockAdd To Cart
Click to expand/collapse details FRET-BFP/GFP-B-NQF-ZERO (-ZERO pixel set mounted in cube)$1,619Ships in 2 daysAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details FRET-BFP/GFP-B-NTE (set mounted in cube)$1,520In StockAdd To Cart
Click to expand/collapse details FRET-BFP/GFP-B-NTE-ZERO (-ZERO pixel set mounted in cube)$1,619Ships in 2 daysAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details FRET-BFP/GFP-B-OMF (set mounted in cube)$1,560In StockAdd To Cart
Click to expand/collapse details FRET-BFP/GFP-B-OMF-ZERO (-ZERO pixel set mounted in cube)$1,659Ships in 2 daysAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details FRET-BFP/GFP-B-OFF (set mounted in cube)$1,560In StockAdd To Cart
Click to expand/collapse details FRET-BFP/GFP-B-OFF-ZERO (-ZERO pixel set mounted in cube)$1,659Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details FRET-BFP/GFP-B-ZHE (set mounted in cube)$1,400In StockAdd To Cart
Click to expand/collapse details FRET-BFP/GFP-B-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,499Ships in 2 daysAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details FRET-BFP/GFP-B-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,574Ships in 2 daysAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Blue
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 3503434413-Star
BFP (EBFP)3804405-Star
DAPI3594613-Star

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
Green
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Alexa Fluor® 4884995203-Star
Cy2™4925073-Star
FITC (Fluorescein)4955193-Star
GFP (EGFP)4895115-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Fluorescence Resonance Energy TransferTHIS MAY BE REMOVED AND USED ONLY IN APPLICATIONS SECTION. Describes the FRET interaction and considerations for choosing filter sets
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
FRET (Flourescence Resonance Energy Transfer)An overview of FRET, including solutions from Semrock and additional learning resources.
Ratiometric ImagingAn overview of ratiometric imaging, including solutions from Semrock and additional learning resources.



FRET-BFP/GFP-B BFP/GFP BFP GFP FRET FF01-387/11 FF01-520/35 FF02-447/60 FF409-Di03 387/11 520/35 447/60 FF409 409 Filter Filters Set Sets FRET-BFP/GFP-B-000 FRET-BFP/GFP-B-000-ZERO FRET-BFP/GFP-B-L01-ZERO FRET-BFP/GFP-B-S01 FRET-BFP/GFP-B-S01-ZERO FRET-BFP/GFP-B-NQF FRET-BFP/GFP-B-NQF-ZERO FRET-BFP/GFP-B-NTE FRET-BFP/GFP-B-NTE-ZERO FRET-BFP/GFP-B-OMF FRET-BFP/GFP-B-OMF-ZERO FRET-BFP/GFP-B-OFF FRET-BFP/GFP-B-OFF-ZERO FRET-BFP/GFP-B-ZHE FRET-BFP/GFP-B-ZHE-ZERO FRET-BFP/GFP-B-LDMK-ZERO

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