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BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: DAPI-5060C-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
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Semrock Filter Set: DAPI-5060C

BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

Brightest DAPI set on the market. This unique "flamethrower" filter set is recommended for those special cases in which it is critical to achieve the highest brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For shorter exposure times this set exhibits up to 5 times more brightness but has lower contrast and more photobleaching than the DAPI-1160B set.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details DAPI-5060C-000 (Standard size set, fits most microscopes2)$785In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-377/50-25$305
Single Band Emitter25 mm x 3.5 mmFF02-447/60-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF409-Di03-25x36$255
Click to expand/collapse details DAPI-5060C-000-ZERO (Standard size -ZERO pixel set)$884In StockAdd To Cart
Click to expand/collapse details DAPI-5060C-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details DAPI-5060C-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.

Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details DAPI-5060C-NQF (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details DAPI-5060C-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details DAPI-5060C-NTE (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details DAPI-5060C-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details DAPI-5060C-OMF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details DAPI-5060C-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details DAPI-5060C-OFF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details DAPI-5060C-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details DAPI-5060C-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details DAPI-5060C-ZHE (set mounted in cube)$1,060In StockAdd To Cart
Click to expand/collapse details DAPI-5060C-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details DAPI-5060C-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details DAPI-5060C-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details DAPI-5060C-AMF-ZERO (-ZERO pixel set mounted in cube)$1,349In StockAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
7-amino-4-methylcoumarin (AMC)3464421-Star
Alexa Fluor® 3503434415-Star
Alexa Fluor® 4054014225-Star
AMCA (Aminomethylcoumarin)3504483-Star
ATTO 3903924773-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804403-Star
Calcofluor White3514443-Star
Cascade Blue™4014193-Star
CellTrace calcein violet4004523-Star
DiFMU-pH 9.03584503-Star
DyLight 3503514343-Star
DyLight 4053984203-Star
ER-Tracker™ Blue-White DPX3725571-Star
FluoSpheres Blue fluorescent microspheres3564143-Star
HiLyte Fluor™ 4054034283-Star
Hoechst 332583524553-Star
Hoechst 333423524553-Star
Hoechst 345803924403-Star
LIVE-DEAD Fixable Blue Dead Cell Stain3434411-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
LysoSensor Blue3744245-Star
LysoTracker Blue3734223-Star
Marina Blue3634615-Star
Pacific Blue™4044555-Star
Quinine sulfate3494613-Star
Vybrant DyeCycle Violet3694373-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.

DAPI-5060C DAPI 377/50 447/60 409 Single-Band High Brightness High-Brightness FF01-377/50 FF02-447/60 FF409-Di03 FF409 Filter Filters Set Sets 1,8-ANS Alexa-Fluor-350 AF350 Alexa350 Alexa-Fluor-405 AF405 Alexa405 AMCA Aminomethylcoumarin ATTO-390 BD-Horizon-V450 BFP EBFP Calcofluor-White Cascade-Blue CellTrace-calcein-violet DyLight-350 DyLight-405 FluoSpheres-Blue Hoechst LysoSensor-Blue LysoTracker-Blue Marina-Blue Pacific-Blue sgBFP TagBFP Vybrant-DyeCycle-Violet DAPI-5060C-000 DAPI-5060C-000-ZERO DAPI-5060C-L01-ZERO DAPI-5060C-U02 DAPI-5060C-NQF DAPI-5060C-NQF-ZERO DAPI-5060C-NTE DAPI-5060C-NTE-ZERO DAPI-5060C-OMF DAPI-5060C-OMF-ZERO DAPI-5060C-OFF DAPI-5060C-OFF-ZERO DAPI-5060C-OFX DAPI-5060C-ZHE DAPI-5060C-ZHE-ZERO DAPI-5060C-LDMP-ZERO DAPI-5060C-LDMK-ZERO DAPI-5060C-AMF-ZERO

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