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BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: DAPI-1160B-000

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Semrock Filter Set: DAPI-1160B

BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving exceptionally low noise (background) yet high brightness, resulting in unsurpassed contrast (signal-to-noise ratio). Images of typical cell samples with DAPI-stained nuclei exhibit more than twice the contrast of those made with the leading competitor's set. The carefully optimized narrow, red-shifted exciter filter minimizes fluorophore photobleaching and sample phototoxicity. What's more, unlike soft-coated filters and absorbing glass filters used for DAPI excitation, the unique hard-coated exciter does not "burn-out" under the intense UV illumination of modern fluorescence light sources.

This set is the unparalleled choice for most situations, especially those involving digital imaging. For specialized applications where brightness trumps contrast, use our DAPI-5060C set, which has the highest brightness on the market.

Individual filters and filter sets (no cube):
These filters sets contain individual filters. For filter sets already mounted in a holder/cube, scroll down to see complete mounted sets with cubes.
Part NumberPrice1Stock StatusQuantity
Click to expand/collapse details DAPI-1160B-000 (Standard size set, fits most microscopes2)$785In StockAdd To Cart
Filter RoleFilter SizePart NumberPrice1
Single Band Exciter25 mm x 5.0 mmFF01-387/11-25$305
Single Band Emitter25 mm x 3.5 mmFF02-447/60-25$305
Single Band Dichroic25.2 mm x 35.6 mm x 1.1 mmFF409-Di03-25x36$255
Click to expand/collapse details DAPI-1160B-000-ZERO (Standard size -ZERO pixel set)$884In StockAdd To Cart
Click to expand/collapse details DAPI-1160B-L01-ZERO (Leica 'Large' -ZERO pixel set)$884Ships in 2 daysAdd To Cart
Click to expand/collapse details DAPI-1160B-U02 (Olympus large-size (FX) set for U-M710I)$1,288Ships in 2 daysAdd To Cart

1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
2) Customers installing filters into their own Nikon Quadfluor cubes should contact us before ordering a filter set.



Complete filter sets mounted in microscope holders/cubes:
These sets are delivered already mounted in microscope filter holders, often called 'cubes', ready to be dropped into your microscope.
Nikon Quadfluor for microscopes: E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
Click to expand/collapse details DAPI-1160B-NQF (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details DAPI-1160B-NQF-ZERO (-ZERO pixel set mounted in cube)$1,279In StockAdd To Cart
Nikon TE 2000 for microscopes: TE 2000, 50i, 55i, 80i, 90i, Eclipse Ti, Ni, and Ci series, and any using the Epi-fluor Illuminator
Click to expand/collapse details DAPI-1160B-NTE (set mounted in cube)$1,180In StockAdd To Cart
Click to expand/collapse details DAPI-1160B-NTE-ZERO (-ZERO pixel set mounted in cube)$1,279In StockAdd To Cart
Olympus U-MF2 for microscopes: AX70, BX, BX41, BX50, BX51, BX60, BX61, BX50/51WI, BX60/61WI, IX50, IX51, IX70, IX71, IX81
Click to expand/collapse details DAPI-1160B-OMF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details DAPI-1160B-OMF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-FF for microscopes: BX53, BX63
Click to expand/collapse details DAPI-1160B-OFF (set mounted in cube)$1,220In StockAdd To Cart
Click to expand/collapse details DAPI-1160B-OFF-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Olympus U-M710I for microscopes: Requires a 32mm emitter & exciter and 32x44mm dichroic beamsplitter. For IX53, IX73, & IX83 model microscopes
Click to expand/collapse details DAPI-1160B-OFX (set mounted in cube)$1,883Ships in 2 daysAdd To Cart
Zeiss FL Cube EC P+C for microscopes: Axio Imager, Axiostar Plus, Axioskop 40, Axioskop 2 (post-2001), Axioplan 2i, Axioplan 2ie, Axiovert 200, Axiovert 40, Axio Observer, Axio Examiner and Axio Scope A1
Click to expand/collapse details DAPI-1160B-ZHE (set mounted in cube)$1,060In StockAdd To Cart
Click to expand/collapse details DAPI-1160B-ZHE-ZERO (-ZERO pixel set mounted in cube)$1,159In StockAdd To Cart
Leica DM-K for microscopes: DM-2000, DM-2500, DM-3000, DMI3000 B, DM-4000, DMI4000 B, DM-5000, DM-5500, and DM-6000, DMI6000 B
Click to expand/collapse details DAPI-1160B-LDMK-ZERO (-ZERO pixel set mounted in cube)$1,234Ships in 2 daysAdd To Cart
Leica DMi8 (P-Cube) for microscopes: DMi8
Click to expand/collapse details DAPI-1160B-LDMP-ZERO (-ZERO pixel set mounted in cube)$1,319In StockAdd To Cart
Aperio AMF for microscopes: ScanScope FL
Click to expand/collapse details DAPI-1160B-AMF-ZERO (-ZERO pixel set mounted in cube)$1,349In StockAdd To Cart


1) US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.
Optical Specifications
SpecificationValue
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
SpecificationValue
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
SpecificationValue
Scratch-Dig60-40
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
1,8-ANS3724773-Star
4-methylumbelliferone3574411-Star
7-hydroxy-4-methylcoumarin3604421-Star
Alexa Fluor® 3503434413-Star
Alexa Fluor® 4054014223-Star
AMCA (Aminomethylcoumarin)3504485-Star
ATTO 3903924773-Star
BD Horizon V4504064493-Star
BFP (EBFP)3804403-Star
Calcofluor White3514441-Star
Cascade Blue™4014191-Star
CellTrace calcein violet4004523-Star
DAPI3594615-Star
DEAC4324721-Star
DiFMU-pH 9.03584501-Star
DY-4154184701-Star
DyLight 4053984203-Star
ECFP4354751-Star
HiLyte Fluor™ 4054034281-Star
Hoechst 332583524553-Star
Hoechst 333423524553-Star
Hoechst 345803924403-Star
LIVE-DEAD Fixable Violet Dead Cell Stain4034553-Star
LysoSensor Blue3744245-Star
LysoTracker Blue3734221-Star
Marina Blue3634615-Star
mBBr+GSH3944901-Star
Pacific Blue™4044555-Star
sgBFP™3874515-Star
Sirius3554241-Star
SpectrumBlue4054491-Star
TagBFP4024573-Star
Vybrant DyeCycle Violet3694373-Star

Tech Note
TopicDescription
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
TopicDescription
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
TopicDescription
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
Application
TopicDescription
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
Alternate Blue Fluorophore Single-Band Sets

DAPI-5060C.png

BrightLine® single-band filter set, optimized for DAPI and other like fluorophores

Highest-brightness "no burn-out" single-band filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP
Part # DAPI-5060C-000
$785
(Non-US pricing info)



DAPI-11LP-A.png

BrightLine® long-pass filter set, optimized for DAPI and other like fluorophores

High contrast long-pass filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP
Part # DAPI-11LP-A-000
$785
(Non-US pricing info)



DAPI-50LP-A.png

BrightLine® long-pass filter set, optimized for DAPI and other like fluorophores

High brightness long-pass filter set. Ideal for use with: DAPI, Alexa Fluor 350, AMCA , BFP , Hoechst 33258, Hoechst 33342, Hoechst 34580, LysoSensor Blue, Marina Blue, Pacific Blue, sgBFP, Fluoro-Gold™
Part # DAPI-50LP-A-000
$785
(Non-US pricing info)


Green Fluorophore Single-Band Sets

GFP-3035D.png

BrightLine® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: GFP , Cy2, CyQuant Cell Proliferation Assay, DiO, EGFP, sgGFP , wtGFP , YOYO-1, YO-PRO-1
Part # GFP-3035D-000
$785
(Non-US pricing info)



FITC-3540C.png

BrightLine® single-band filter set, optimized for FITC and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: FITC , 5-FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green, Oregon Green, Rhodamine 110, rsGFP
Part # FITC-3540C-000
$785
(Non-US pricing info)


Orange Fluorophore Single-Band Sets

TRITC-B.png

BrightLine® single-band filter set, optimized for TRITC and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: TRITC , Alexa Fluor 546, 5-TAMRA , Alexa Fluor 532, SpectrumOrange, BODIPY TMR-X, DiI, POPO-3, PO-PRO-3, Rhodamine
Part # TRITC-B-000
$785
(Non-US pricing info)



Cy3-4040C.png

BrightLine® single-band filter set, optimized for Cy3 and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: Cy3, DsRed, Alexa Fluor 555, Calcium Orange, LysoTracker Yellow, MitoTracker Orange, Phycoerythrin, Rhodamine Phalloidin, 5-TAMRA
Part # Cy3-4040C-000
$785
(Non-US pricing info)


Red Fluorophore Single-Band Sets

Cy5-4040C.png

BrightLine® single-band filter set, optimized for Cy5 and other like fluorophores

High brightness and contrast single-band filter set. Ideal for use with: Cy5, SpectrumFRed - Lipophilic Tracer, SYTO Red Fluorescent Nucleic Acid Stains 60, 62, 63, TOTO-3, TO-PRO-3
Part # Cy5-4040C-000
$785
(Non-US pricing info)


[x]
US domestic pricing only. If you are ordering from outside the US, please contact your nearest regional distributor for the correct list price.



DAPI-1160B DAPI 387/11 447/60 409 Single-Band High Contrast High-Contrast FF01-387/11 FF02-447/60 FF409-Di03 FF409 Filter Filters Set Sets 1,8-ANS Alexa-Fluor-350 AF350 Alexa350 Alexa-Fluor-405 AF405 Alexa405 AMCA Aminomethylcoumarin ATTO-390 BD-Horizon-V450 BFP EBFP CellTrace-calcein-violet DyLight-405 Hoechst Hoechst-33258 Hoechst-33342 Hoechst-34580 LysoSensor-Blue Marina-Blue Pacific-Blue sgBFP TagBFP Vybrant-DyeCycle-Violet DAPI-1160B-000 DAPI-1160B-000-ZERO DAPI-1160B-L01-ZERO DAPI-1160B-U02 DAPI-1160B-NQF DAPI-1160B-NQF-ZERO DAPI-1160B-NTE DAPI-1160B-NTE-ZERO DAPI-1160B-OMF DAPI-1160B-OMF-ZERO DAPI-1160B-OFF DAPI-1160B-OFF-ZERO DAPI-1160B-OFX DAPI-1160B-ZHE DAPI-1160B-ZHE-ZERO DAPI-1160B-LDMP-ZERO DAPI-1160B-LDMK-ZERO DAPI-1160B-AMF-ZERO

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