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BrightLine® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

Semrock Set / Fluorophore Graphing

Part Number: GFP-4050A-000

Fluorophore Displayed:
Please select your fluorophore from the Fluorophore Tab below
Left-click & drag to zoom. Right-click to save, print or reset

Optical Specifications
BlockingBrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

General Filter Specifications
Microscope CompatibilityBrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Damage RatingTesting has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

Physical Filter Specifications
Exciter/Emitter OrientationArrow on ring indicates preferred direction of propagation of light.
Dichroic Orientation"Reflective coating side" should face toward light source and sample.

5-Star Optimized – Filter set is specifically optimized for that fluorophore.
3-Star Compatible – Should result in nearly ideal performance in most situations.
1-Star Acceptable – Actual performance is dependent on specific experimental conditions.
FluorophorePeak Excitation
Wavelength (nm)
Peak Emission
Wavelength (nm)
CompatibilityDisplay on
Plot above
Acridine Orange (+DNA)5025261-Star
Acridine Yellow4705501-Star
Alexa Fluor® 4304315411-Star
Alexa Fluor® 4884995201-Star
ATTO 4885005251-Star
ATTO 4954955271-Star
BCECF (pH 5.5)4815183-Star
BCECF (pH 9.0)5025281-Star
CFP (ECFP)4334751-Star
CyQuant Cell Proliferation Assay5025233-Star
CyQUANT GR-DNA5025231-Star
Dendra2 (Green)4915073-Star
ecliptic pHluorin pH5.54735073-Star
FITC (Fluorescein)4955191-Star
Fluorescein dextran5015241-Star
Fluorescein-pH 8.04895171-Star
FM 1-434735791-Star
GFP (EGFP)4895115-Star
Green 4964965201-Star
Green 5005015241-Star
HCS LipidTOX Green phospholipidosis5045361-Star
HiLyte Fluor™ 4884975261-Star
LIVE-DEAD Fixable Green Dead Cell Stain4985251-Star
Lucifer yellow4285441-Star
LysoSensor Green4485023-Star
LysoTracker Green5015091-Star
LysoTracker Yellow HCK-1234885651-Star
MitoTracker™ Green4905123-Star
NBD-X (MeOH)4675383-Star
NeuroTrace 500/525 Green Fluorescent Nissl Stain4955241-Star
Oregon Green™ 4884985261-Star
Pro-Q Emerald2895303-Star
Qdot® 525 Nanocrystals3005251-Star
Qdot® 545 Nanocrystals3005431-Star
Rhodamine 1104975191-Star
Rhodamine Green4975231-Star
Rhodol Green4975241-Star
sgGFP™ (super glow GFP)4725063-Star
Sodium Green5075321-Star
SYBR Gold nucleic acid gel stain-DNA4965391-Star
SYBR Green I nucleic acid gel stain-DNA4985221-Star
SYTO 134485063-Star
SYTO 164895203-Star
SYTO 454514851-Star
SYTO 94835003-Star
SYTO RNASelect geen fluorescent cell stain5035271-Star
SYTOX Green-DNA5045241-Star
Vybrant DyeCycle Green5065341-Star
wtGFP (wild type GFP, non-UV excitation)4745093-Star

Tech Note
Cleaning Optical FiltersInstructions for cleaning hard coated optical filters
Cube Assembly InstructionsPDF and video instruction for installing filters into popular filter cubes
Filter ReliabilityCompares the performance and reliability of hard coated filters to traditional soft-coated filters and provides information about the testing standards for Semrock filters
Introduction to Fluorescence FiltersShows how optical filters are used in a traditional fluorescence microscope and describes important factors to consider when choosing optical filters.
Orientation of Filters in a MicroscopeA guide to properly orienting Semrock filters based on the filter markings.
What is Pixel Shift?Explains how filter wedge angle causes pixel shift and describesthe impact of pixel shift on image fidelity
Journal Article
Achieving the Best Alignment for Fluorescent ImagesTuran Erdogan, PhD, and Atul Pradhan, PhD, Semrock Inc., BioPhotonics, August 2005
Semrock's BrightLine ZERO filter sets eliminate the beam deviation that can cause pixel shift when combining single-color images to create a multicolor image
White Paper
Spectral Modeling in Fluorescence MicroscopyDiscusses various sources of noise in fluorescence microscopy and how to maximize signal-to-noise ratios using software, sample preparation and optical filter selection
High Performance MicroscopyAn overview of high performance microscopy, including solutions from Semrock and additional learning resources.
Revised Filter Set


BrightLine® single-band filter set, optimized for Green Fluorescent Protein and other like fluorophores

Highest-brightness single-band filter set. Ideal for use with: GFP , Cy2, CyQuant Cell Proliferation Assay
Part # GFP-4050B-000
(Non-US pricing info)

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